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Comparison of HPLC a GC-MS method for pesticide determination in soil
Chalbia Václavíková, Alena ; Kočárek, Martin (advisor) ; Havlík, Jaroslav (referee)
Physical and chemical properties of pesticides may vary significantly. Pesticides can be acidic, neutral or alkaline. Some compounds containing phosphorus, other sulfur, nitrogen or halogens. These atoms can be important for the detection of pesticides. Some of the compounds are volatile, others on the contrary not evaporate, which is also one of the important properties for chromatography. The diversity of pesticides actually makes it impossible to create a universal method, which is very impractical in terms of quality and time determination. Because that pesticides are one of the most chemicals in most countries. In the food and drinking water are limits on pesticides in order to avoid negative impact on public health. For residues in soil or surface water limits are set. (Lutz et al., 2006)
The objective of this thesis is to evaluate the possibility of using two types of chromatographs with different detectors for the analysis of pesticide. Pendimethalin which was chosen for this work, will be analyzed usinga liquid chromatograph with UV detektor and gas chromatography with mass detector. Both this method will be compared as well two extraction methods (Soxhlet extraction and shaking), and two extraction agents (methanol and acetonitrile).
Another point of the work is to provethe hypothesis that, for the determination of pendimethalin in soil is by gas chromatography using a mass spectrometer in comparison with the high performance liquid chromatography time and more expensive, but will achieve a higher yield and a lower detection limit of the pesticide.
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Supplier management in automotive industry
Holubcová, Romana ; Štůsek, Jaromír (advisor)
In my thesis I deal with the process of Supplier management in automotive industry. Supplier - customer relationship is very important for a success both company and for the global development of the economy too. These relationships must be partnership and support the development of the companies and their each other businesses are beneficial for both sides. In my thesis I analyze current process of the supplier management in the company, what directly supply to OEM. Original Equipment manufacturing (OEM), is the final producer of automobiles, it is mean this company do not produce components but assemble supplied modules. I propose improvements and up-date the directive Strategic purchasing according to results from the analysis.
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Genetic eye diseases in dogs
Šmejkalová, Barbora ; Hofmanová, Barbora (advisor)
Eye diseases in dogs are often very similar to humans eye disease. Eye diseases are becoming more common in pure breed of dogs, therefore it is important to take them into consideration, particularly when we are crossing the parent generation.
In the first part is explained in detail and illustrated anatomy of the eye, including images and photographs. In the next part, there are described various eye diseases, namely cataract, glaucoma, hereditary diseases of the retina, progressive retinal atrophy (PRA) and Collie Eye Anomaly (CEA). For each disease is described, how it is possible to diagnose the disease, how it progress, what is its connection with genetic and possible treatment alternatives. In closing there are tables and graphs showing the frequency of occurrence of a specific disease either from an overall perspective or from the perspective of individual breeds.
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Electronic auction in healthcare
Vyoral, Marek ; Toth, Štefan (advisor) ; Jan, Jan (referee)
Working with the theoretical part focuses on the regulation of electronic auctions in terms of legislation both Czech and foreign, given that we are members of the European Union. Furthermore, the work explains the possible ways to use electronic auctions, which are mostly used in both the Czech Republic and abroad. At work I also am trying to shed light on the current use of auctions on the auction portal Aukro examples, which is widely used by people for personal use. Finally, the theoretical part I try to outline the future development of electronic auctions. The practical part is focused primarily on its activities in the hospital Pardubice Region, Inc. where I introduced the auction. In several examples, visible results, which not only brings centralization entire purchase, but also the use of electronic auctions as a tool for communication and bidding suppliers with the lowest purchase price for a group of hospitals. The work also draws from the Ministry for Regional Development, which is responsible for the preparation of the most important regulations for electronic auctions in healthcare, namely the Public Procurement Act. Electronic auctions are a tool very interesting, because it is the most transparent way of evaluating procurement by parameters rates. Whether if electronic auction in health care and efficient purchase option will be discussed in this work.
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Revitalization of the chateau park in Choltice
Čermáková, Michaela ; Vaněk, Jan (advisor) ; František, František (referee)
This work has the task to assess the current situation of the chateau park and at the same time the game preserve in Choltice, and suggest its revitalization in order to rejuvenate the vegetation and making the field available for the general public. Choltice is a market town in the Pardubice region, approximately 15 km west from Pardubice. At the end of 17. century a baroque castle was built here, which belonged to the count Simon Thun. His family belonged it until 1945. The chateau and the park is now owned by the borough Choltice. The chateau neighbours with the extensive English park, which passes the chateau game preserve, which is since 1992 a nature reserve and an important European locality.
The diploma thesis contains a detailed theoretical basis and proposes solutions, which are directed both on the protection of the living conditions of protected and endangered species of a beetle Osmoderma eremita. This species occurs only rarely and his survival area is very limited, therefore it is necessary to create those conditions, and it is currently one of the main tasks of the revitalization.
The next step is to help revitalize the introduction of spontaneous breeding fallow deer in the game preserve. This idea is not new, the fallow deer were bred there until 1945. The town of Choltice is very inclined towards this idea about farming and supports it. To increase the number of visitors this diploma thesis contains a proposal for the creation of a nature trail, this area would attract wider public than so far.
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Supplier management in automotive industry
Holubcová, Romana ; Štůsek, Jaromír (advisor)
In my thesis I deal with the process of Supplier management in automotive industry. Supplier - customer relationship is very important for a success both company and for the global development of the economy too. These relationships must be partnership and support the development of the companies and their each other businesses are beneficial for both sides. In my thesis I analyze current process of the supplier management in the company, what directly supply to OEM. Original Equipment manufacturing (OEM), is the final producer of automobiles, it is mean this company do not produce components but assemble supplied modules. I propose improvements and up-date the directive Strategic purchasing according to results from the analysis.
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Resistance of cole vegetable cultivars against Plasmodiophora brassicae pathotypes in Czech Republic
Zacharová, Lea ; Kazda, Jan (advisor) ; Gerhard, Gerhard (referee)
Clubroot disease of crucifer plants is caused by a pathogen Plasmodiophora brassicae (Wor.). It is counted among the most common and most serious diseases that attack not only rape, but also, for example, head cabbage, cauliflower, broccoli and other kinds of cabbage vegetables.
Plasmodiophora brassicae is descirebed as a very durable and well-developed body in this thesis. Physical, chemical and biological components of the soil environment are in the thesis associated with an effect on survival, growth and reproduction of this microbe. Obviously, Plasmodiophora's brassicae successfulness is given due to its resting spores and because of them the microbe is able to survive in a soil for years.
The experimental part of my thesis is focused on a testing of resistance of selected cultivars against Plasmodiophora brassicae. Each variety were grown under the control within conditions in the greenhouse. To evaluate the degree of a damage of the plants scale of Buzacki (1975), Strelkov et al. (2007) were applied, the index of an invasion was calculated based on these scales.
Cultivars were exposed to a pathotype 6 and as a control variety was used the cultivar Granaat. The results had shown the most susceptible respond of a cultivar red cabbage F1 Loder and other varieties tested had confirmed their resistance to Plasmodiophora brassicae.
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Freshwater mussels breeding methods for the purpose of conservation programs
Plechingerová, Věra ; Douda, Karel (advisor) ; Matasová, Klára (referee)
Bachelor thesis is written in an overview form of problems breeding freshwater molluscs. At the beginning of the thesis I first evaluate the overall status of the populations in the Czech Republic and then in the world. Next I describe the anatomy and biology of molluscs, especially their reproduction, which is crucial for rearing juveniles in the laboratory or seminatural environment. The return of molluscs populations into the waters ecosystems is problematic in terms of ongoing changes in the biotope, particularly because of the anthropogenic influences and too few suitable host fish, without which it can not complete its glochidia development. To prevent the complete extinction of some species, there have been researched different methods of rearing, which help recover viable populations in the natural water environment. Methods of rearing are also described in this thesis. In the experimental part of the thesis I evaluate types of detritus that have different effects in length gain of mussels. For the experiment was selected freshwater pearl mussel (Margaritifera margaritifera), whose population is highly endangered. Freshwater mussels are an important bio-indicator of waters ecosystems, where they have an irreplaceable function, so it is important to regenerate their populations and the overall condition of the biotopes.
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Realization of farm with a focus on poultry
Hromjak, Milan ; Zita, Lukáš (advisor) ; Kovářová, Kateřina (referee)
This thesis deals with the origin of a family farm. By creating a business plan and issues connected with it, its priority is an organic poultry farm, which will produce poultry meat and eggs. Another service beyond the farm production will be creating conditions for a developing branch, which is agro-tourism and other services related to the operation of the farm, so that it leads to the economic development and profit.
The farm will arise by rebuilding of an old house with a large barn and adjacent 10ha of lands inherited from grandparents. There will be built a multi-purpose building called the poultry farm from which the poultry will have access to pasture. The property includes another 1 ha of forest from which the wood will be used as a building material and firewood.
The work is divided into two parts: theoretical and practical. The theoretical part especially focuses on explaining the concepts that are important to understand the business plan.
Then there is the legislation concerning the organic farming, a tangle of laws, public notices and regulations that must be followed in order to meet the conditions for the organic farming. An integral motivational service of the country and the European Union for farmers and not only for those who are environmentally-managing farmers, is support and grants for the years 2014 - 2020. A very important step not only for the starting farmers is a market analysis in the given branch, as well as a SWOT analysis that will show us the positive and negative aspects of the business. The conclusion of this section deals not only with the concept of agro-tourism, but also with its market position as well and possibilities of its use and further development.
In the practical part there is a description of a business plan and individual periods of the project including financing. Finances will flow from own and foreign capital, or the possibility to apply for support through non-refundable grants. The establishment of the farm will have an impact on the labor market, as there will be 5 newly created jobs and the community will fit into the wider public awareness thanks to the activities that the farm will develop for the purpose of product promotion and further development. Furthermore, there are itemized individual items that lead to the final evaluation of the effectiveness of the business plan. By processing the theoretical and practical part of the thesis the requirement of the thesis objective is met - to design a prosperous family farm focused on poultry keeping with a hypothesis if it is currently possible to be competitive while importing poultry meat and eggs from abroad. It can be concluded that the business plan is viable.
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Use of Festuca arundinacea for decorative lawns
Doskočil, Jan ; Svobodová, Miluše (advisor) ; Hrevušová, Zuzana (referee)
Use of Festuca arundinacea for decorative lawns
Summary
Festuca arundinacea is briefly bentgrass with broader and coarser leaves. Hardly perennial grass, native to Europe, is well known for its high resistance of dry weather, low intensity of maintenance and resistance of high use. These qualities gains thanks to its well-developed and deep root system, which is capable to gain water and nutrients from deeper parts of soil. Its use is in places with high use, like a race-course, edges of roads or vineyards alleyway. Today, in time of global warming, its usage becomes more important for park purposes and low-input turfs, where is its higher resistance against biotic and abiotic stresses, mainly against drought, utilized. Festuca arundinacea complement well with Poa pratensis in turf.
Goal of this work was to evaluate turfs with Festuca arundinacea and chosen grass species under different mowing frequency. According to hypothesis frequency shouldn´t affect turf coverage. Coverage should be same with different mixture composition with Festuca arundinacea.
The experiment was performed on experimental land of Czech university of Life Sciences Prague, Suchdol in 2015. Mixtures of Festuca arundinacea with Festuca rubra, Poa pratensis and Lolium perenne were seeded together with monocultures of these species in 2012. Mixture composition was 50/50 except mixture of Fesruca arundinacea and Poa pratensis, where the mixture was 75/25. There were conducted 63 plots, in 3 repetitions and in 3 different frequencies of mowing (by 14, 30 and 45 days). Each plot was 6 m2 big. The results were evaluated by analysis of variance ANOVA (P is smaller than 0.05) in program Statgraphics, version XV.
Influence of mixture composition to level of growth was significant. The highest level of growth has monoculture of Festuca arundinacea (10,6 cm). The lowest level of growth has monoculture of Lolium perenne (5,4 cm). Monoculture of Festuca arundinacea had the highest intensity of grow (0,07 cm/day) at 14-day frequency of moving. Lolium perenne has the lowest intensity of grow (0,02 cm/day). At 30-day frequency of mowing mixture of Festuca arundinacea with Poa pratensis has the highest intensity of grow (0,24 cm/day). The lowest intensity of grow has Lolium perenne (0,01 cm/day). The highest intensity of grow was at 45-day mowing frequency. The highest intensity of grow has mixture of Festuca arundinacea with Festuca rubra (0,75 cm/day). The lowest intensity of grow has monoculture of Lolium perenne (0,06 cm/day). Influence of mixture composition to coverage was statistically significant. The highest coverage has mixture of Festuca arundinace with Festuca rubra (81,3 %). The lowest coverage has monoculture of Lolium perenne (58,6%). Influence of frequency to level of growth was statistically significant. The highest level of growth has 45-day frequency of mowing (6 cm) and the lowest level of growth has 14-day frequency of mowing (3,8 cm). Influence of frequency to coverage was statistically significant. The highest coverage 14-day frequency of moving (76,5 %) and the lowest has 45-day frequency of growing (66,7 %).
The knowledge gained will be used for further compilation grass mixtures for park lawns. It turned out that it is better to prefer seeding mixtures with Festuca arundinacea than monocultures. To achieve high coverage and optimal intensity of growth with Festuca arundinacea was recommended to follow 30-day frequency of mowing.
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