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Molecular Markers with Impact on Kidney Graft Survival and Glomerulopathies Progression
Brabcová, Irena ; Viklický, Ondřej (advisor) ; Jirsa, Milan (referee) ; Rychlík, Ivan (referee)
The progression of chronic glomerulopathy and graft rejection is affected by a number of proinflammatory cytokines, whose role in the pathogenesis of damage is poorly understood. The aim of this dissertation was to identify reliable risk markers of renal dysfunction progression and thereby contribute to a more effective patient treatment. Human native kidney biopsies with histologically confirmed diagnosis of glomerulopathy or kidney graft biopsies were analysed. Intrarenal gene expressions were measured by RT-qPCR. Single nucleotide polymorphisms were detected by methods based on PCR-RFLP. Immunohistochemical staining was used to identify and quantify the mononuclear cell infiltration. Gene expression of TGF-β1, HGF, BMP7, MCP-1, RANTES and mononuclear cell infiltration were associated with poor renal function and proteinuria at the time of IgA nephropathy diagnosis. Progression of IgA nephropathy during the 2-year follow-up was shown to be dependent on the degree of chronic vasculopathy and TGF-1 expression in the kidney. Patients with graft dysfunction and enhanced intrarenal expression of TGF-1, MCP-1 had significantly shorter graft survival. Higher mRNA expression of IL-10, TGF- 1, IL-6, MCP-1, RANTES and TNF- was observed in patients with graft dysfunction presented at the time of biopsy....
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Immunotherapy of ovarian carcinoma with dendritic cells
Partlová, Simona ; Rožková, Daniela (advisor) ; Froňková, Eva (referee)
V ANGLICKÉM JAZYCE Immunotherapy of ovarian carcinoma with dendritic cells Anticancer immunotherapy is a therapeutical strategy aimed at elicitation and maintenance of immune responses against cancer cells. In this study we have focused on immunotherapy of ovarian cancer, because it is one of the most common gynaecological tumors with poor prognosis and high mortality. Our immunotherapy protocol involves preparing dendritic cells (DC) from monocytes isolated from patient's peripheral blood, which are subsequently pulsed with irradiated cells of established ovarian cancer cell line. These immature pulsed DC are maturated and subsequently co-cultivated with autologous T lymphocytes. The aim of this study was to demonstrate, that DC are able to elicit specific immune response after addition of suitable mature agens in combination with apoptotic ovarian tumor cells. Our observations indicate that 24 hours are sufficient for induction of tumor cells apoptosis. Additionally, we have shown that DC successfully ingested most of the apoptotic tumor cells after 4 hours of co-incubation. Furthermore, we have found out that ingestion of apoptotic cells by dendritic cells, which are stimulated with polyI:C, inhibits maturation of DC and consequently also production of cytokines IL-12p70, IL-6 and TNF-α. Whereas...
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Importance of trombophilic mutations in clinical genetic.
Vavrušková, Klára ; Kuklík, Miloslav (advisor) ; Černá, Marie (referee)
Trombophilia means an increased disposition to creation of trombs. Health complication incurred as a consequence of hypercoagulation can be very serious. When a trombophilic mutation is found at a patient, it brings necassity of thrombosis - control in risk situations (e.g. pregnancy, operation) for the rest of the patient's life. There were filed 300 people (206 women and 94 men) with trombophilic mutations into my study of clinical signification of trombophilic mutations. These people were examinated in years 2008 - 2010. Most of positive medical findings - 266 people, were recorded in the area of MTHFR (C677T i A1298C) mutations. There were less findings in the field of FV Leiden and FII prothrombin mutations. Multipath trombophilic mutations were found at 99 patients. I accordance with foreign literature, our results advert to clinical consequences of trombophilic mutations like: repeated spontanious aborts, cerebrovascular akcident (CA), ischaemic heart disease (IHD), thrombosis, flebothrombosis, pulmonary embolism, varicose veins, aseptic necrosis of hip bone, arterial sclerosis and aortic stenosis. Mutations MTHFR C677T and MTHFR A1298C we found mainly at patients with CA, IM and IHD. Leiden mutation was most often found at patients with thrombosis, flebothrombosis and pulmonary embolism. We...
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The influence od educational to maintenance of secondary prevention rules of myocardial infarction
ŠÍMOVÁ, Martina
This bachelor thesis deals with the effects of educational plans on the compliance with the principles of the secondary prevention of myocardial infarction. The theoretical background covers atherosclerosis, acute myocardial infarction, the secondary prevention of myocardial infarction and the educational process in nursing. Prior to elaborating the practical part of the thesis we had established two objectives. Objective 1: To find out whether the patients educated without an educational plan are as knowledgeable as the patients educated through an educational plan. Objective 2: To compare whether the patients educated without an educational plan comply with the principles of the secondary prevention of myocardial infarction in the same way as the patients with an educational plan. A qualitative form of research had been chosen for this thesis, semi-standardized interview in particular. The analysis of the data gained was subsequently carried out. Two objectives had been established in the thesis. Objective 1: To find out whether the patients educated without an educational plan are as knowledgeable as the patients educated through an educational plan. Through the analysis of the obtained data we found out that the patients educated without an educational plan are as knowledgeable as the patients educated through an educational plan. Objective 2: To compare whether the patients educated without an educational plan comply with the principles of the secondary prevention of myocardial infarction in the same way as the patients with an educational plan. Having compared the results we found out that the patients with an educational plan comply with the principles of the secondary prevention more consistently than the patients educated without an educational plan. The objectives of the thesis were met.
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Neurorehabilitation after brain injury. Therapeutic possibilities.
Angerová, Yvona ; Lippertová-Grünerová, Marcela (advisor) ; Jeřábek, Jaroslav (referee) ; Kozler, Petr (referee)
Neurorehabilitation is a multidisciplinary rehabilitation process used in patients with neurological diseases. These patients have not only movement disorders but also cognitive and neurobehavioral problems as well as aphasias. Their rehabilitation is a long term process and the results are often unsatisfactory. Neuroplasticity - physiological basis for neurorehabilitation induces functional restitution or recovery after secondary brain damage. Various neuroprotective substances (e.g. erythropoietin - EPO) are tested to empower mechanisms of plasticity after brain injury. Preclinical studies testing efficacy of those substances in animal brain damage models are essential to prepare clinical trials. The aim of the study was to reveal the influence of EPO combined with rehabilitation on functional outcomes after global cerebral hypoxia. FIM (Functional independence measure) test was used for functional evaluation and Meilli test for visual memory of the patients who attended special program for rehabilitation in clinical part. Patients who came earlier had better prognosis than patients who came later. In experimental part three-months old male Wistar albino rats were exposed to hypobaric hypoxia for 60 minutes in an experimental chamber, simulating an altitude of 8000 m. Half of the animals received...
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Effect of a comprehensive rehabilitation program of children with asthma bronchiale
Vránová, Eva ; Hošková, Blanka (advisor) ; Majorová, Simona (referee)
Title: Effect of a comprehensive rehabilitation program of children with asthma bronchiale Objectives: The main objective of this thesis is to show the usefulness and necessity of physical therapy in children with bronchial asthma and how does it affect them. Another objective was to summarize the possible available methods with demonstrations of examples of individual exercises that can be used for this disease. The aim was also monitoring and evaluation of the patient's subjective feelings. Methods: In the thesis we carried out a single arm experiment, where we used comprehensive kinesiology analysis of individual subjects and the methods of lung function tests. From the lung function test, we chose the spirometry test by method of curve flow / capacity and also we used the peak expiratory flow monitoring using peak-flow-meter. We compared the subjective feelings of clients at the beginning and the end of the treatment program. In the period we watched the changes of value in individual measuring techniques. Results: We found that a comprehensive treatment of children asthma has a significant impact on their posture and also on the respiratory functions and subjective perception of individual symptoms investigated clients. All clients received the same treatment and each of them occurred in...
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Stress factors affecting operators of emergency call 112 and their prevention at chosen workplaces
DVOŘÁKOVÁ, Kamila
This thesis is dealing with the issues of stress and stress factors found at the 112 emergency call operators. Citizens call the emergency number 112 in case of notification of an extraordinary situation. However, in some cases the emergency call is being misused. The call is then marked as spiteful. The ECC runs in a 24-hour shift operation and the operators change after a 12-hour shift. Each new operator is accepted in the service based on his knowledge, physical, health and above all psychological examination. He is obliged to attend the entrance training and courses in lenght of overall nine weeks. There he gets to know the issues of FRS CR, operational management and he has to pass a language course as well. It is very psychologically demanding for every operator to receive emergency calls. The operator needs to be able to listen, empathize and react adequatly. He deals with calls concerning accidents, injuries, foreigners and spiteful calls. An operator should recognize its gravity and devote to its resolution. However, he has his own personal life along with its joys and problems. It is vital that he is able to separate issues of his work and those of personal nature. This and many other indicators may cause stress reactions to the operator. Many psychologists have attempted to define stress. Each has, however, a different opinion and it is complicated to reach a an accurate definition. One thing is for sure - and that is stress shows itself in different ways and everyone is affected by it differently. In case of an emergency call operator, the stress factors are mainly time to execute the call, character of the notification, as well as the working group, personal life and emotional challenge. Multiple questions have been formulated for this thesis. Both current and former emergency call operators in Ceske Budejovice have been asked these question, which were then processed. The questions were divided into five fields concerning the interviewees' characteristics, collective and family, prevention a one last field was designated for the former operators. The interviews were anonymous, recorded and summarized. Two objectives were defined for this thesis: an analysis of the stressful situations of the emergency call operators and also of the impulses causing the formation of the stress factors, aw well as drafting a solution to stressful situations, possibility of the psycho-training, motivation and relaxation. The answer to the research question 'Which stress factors restrict the operators while performing service and how do they come into being' was determined from the carried-out interviews. There are situations which can make the operators insecure, such as a suicidal call, or when the call is unintelligible, or in case the incident cannot be correctly and quickly localized. Another problem can be the spiteful calls, in which case the telephone just rings and the caller hangs up right away. Another answer has also been extracted from the respondents: 'Which possibilities to handle stress do the operators have, which way to get rid of stress is satisfactory for them'. In the area of FRS of the South Bohemian Region there is a sports ground for every member to use. They would gladly welcome an opportunity to visit the firefighters's intervention in person. They are interested in psychology education, working with a suicidal call and periodic language courses. There are some proposals and ways to prevent stress of the 112 emergency call operators stated in the conclusion of the thesis. The system of training, possibilities to exchange workplaces, periodic language courses and interviews with the regional psychologist of the FRS of the South Bohemian Region. There are relaxation methods introduced, which would enable the operators to reduce stress and thus prevent illnesses, psychic exhaustion and eventually even a burnout. The results of this thesis can be used for all 112 EECs in the Czech republic.
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