|
Modern reverse stationary phases based on silicagel, zirconium dioxide and organic monoliths: application to analysis of biologically active compounds
Soukupová, Klára
Charles University in Prague Faculty of Science Department of Analytical Chemistry Modern Reversed Stationary Phases Based on Silicagel, Zirconium Dioxide and Organic Monoliths; Their Used in Separation of Biologically Active Compounds Synopsis of PhD. Thesis Prague 2008 Klára Soukupová The PhD. Thesis was carried out at the Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, in the period of 2003-2008. Author: Mgr. Klára Soukupová Supervisors: RNDr. Jana Suchánková-Sobotníková, Ph.D Doc. RNDr. Eva Tesařová, CSc. Prague 2008 1 Table of contents 1. Introduction 2 2. Objectives of the Thesis 3 3. Results and Discussion 4 4. Conclusions 12 5. References 13 Appendix 1: List of Publications, Lectures and Poster presentations 14 Appendix 2: Curriculum Vitae 16 2 1. Introduction High performance liquid chromatography (HPLC) is modern instrumental method used mainly in analytical chemistry. Most used separation mode is reversed phase liquid chromatography (RP HPLC). This method is very useful because of high separation efficiency, reliability and wide choice of stationary phases with different selectivity. Most used stationary phases in RP HPLC are bonded phases, which have many advantages: good availability, wide application range, fast establishment of equilibrium and ability to...
|
|
Modern reverse stationary phases based on silicagel, zirconium dioxide and organic monoliths: application to analysis of biologically active compounds
Soukupová, Klára ; Sobotníková, Jana (advisor) ; Pacáková, Věra (referee) ; Cvačka, Josef (referee)
Charles University in Prague Faculty of Science Department of Analytical Chemistry Modern Reversed Stationary Phases Based on Silicagel, Zirconium Dioxide and Organic Monoliths; Their Used in Separation of Biologically Active Compounds Synopsis of PhD. Thesis Prague 2008 Klára Soukupová The PhD. Thesis was carried out at the Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, in the period of 2003-2008. Author: Mgr. Klára Soukupová Supervisors: RNDr. Jana Suchánková-Sobotníková, Ph.D Doc. RNDr. Eva Tesařová, CSc. Prague 2008 1 Table of contents 1. Introduction 2 2. Objectives of the Thesis 3 3. Results and Discussion 4 4. Conclusions 12 5. References 13 Appendix 1: List of Publications, Lectures and Poster presentations 14 Appendix 2: Curriculum Vitae 16 2 1. Introduction High performance liquid chromatography (HPLC) is modern instrumental method used mainly in analytical chemistry. Most used separation mode is reversed phase liquid chromatography (RP HPLC). This method is very useful because of high separation efficiency, reliability and wide choice of stationary phases with different selectivity. Most used stationary phases in RP HPLC are bonded phases, which have many advantages: good availability, wide application range, fast establishment of equilibrium and ability to...
|
|
Solvability of increase of the season's budget in hockey club
Marx, Antonín ; Malecha, Petr (advisor) ; Oponent B, (referee)
Graduation theses called "Solvability of ice hockey club's seasonal budget increasing" is proposing introduce practical suggestion for 1. League hockey club to solving financial crisis. Afterwards, among other goals is submitting of potential partners list to hockey club, analysis of contemporary communication strategy and creation of a new communication strategy. In the theoretical part will be single notions and instruments taken to parts pertaining to subject matter of this task. In the analytic part will be used methods, which partly bring doser status quo of club's communication possibilities as well as method, which will submit overview of club's present circumstances to us. In the closing part will be on the basis of theoretical solutions and single analysis of the present state compiling a new communication strategy, implementing an application of this strategy as well as club's stock list creating with suggestion of factual partners, which could accost a club.
|
|
ANP methods in brewing industry
Geschmay, Pavel ; Fiala, Petr (advisor) ; Witz, Karel (referee)
Thesis applies methods of ANP in brewing industry. It concentrates on Czech beer market and small local brewery in Policka. Main goal of the thesis is to apply the methods in real life problems from the chosen segment. Work consists of several chapters. First chapter defines the goals of the thesis and describes situation in Policka brewery. Second deals with theory around ANP processes. Third chapter is the main part of the thesis. It applies the theory by solving concrete problems. Last chapter analyzes the results.
|
|
A study of aneuploidy in gametes and embryos
Diblík, Jan ; Macek, Milan (advisor) ; Forejt, Julius (referee) ; Rubeš, Jiří (referee)
The thesis deals with improvement and clinical application of molecular cytogenetic methods for reproductive genetics. These methods include both clinical investigations used for improvement of diagnostic and therapeutic care for infertile couples and experimental methods that can become the basis of new diagnostic tools. The thesis concentrates on the study of aneuploidies, because they constitute a major complication of human reproduction especially by means of assisted reproductive technologies. Aims The main practical aim was the introduction of fluorescence in situ hybridization (FISH) for evaluation of chromosomes in sperm, polar bodies and blastomeres for prefertilisation and preimplantation diagnosis of aneuploidies. The main scientific objective was the study of chromosome localization in nuclei of blastomeres, that are removed from human embryos for preimplantation diagnosis. The aim of this study was to find, whether the localization of chromosomes in relation to the nuclear center and periphery is ruled by the same rules as in other cell types in later stages of development and whether the localization is influenced by aneuploidy. Next aim was to search for peripheral localization of chromosome X in embryos with more than one copies of the chromosome X. This could be a manifestation of X...
|
|
Modification of a system for managing knowledge of workers focused on SW development
Šimková, Marie ; Klas, Jan (advisor) ; Stinka, Vít (referee)
Knowledge management is one of frequently discussed disciplines. Knowledge in business area became the main competitive advantage in the last years and therefore it is necessary to manage knowledge effectively. This paper is focused on analysis of a system for managing knowledge used in a big software company and on suggestion of possible modifications of chosen parts of this system, which is the overall goal of this paper. Concrete goals are defined within the analytical part of this paper.
|
|
Depth Map Estimation from Stereo Images
Berek, Daniel ; Hradiš, Michal (referee) ; Španěl, Michal (advisor)
This thesis is dedicated to the problem of stereo vision and 3D reconstruction from stereo image. Spatial perception is realized by using depth map, which is created by using disparity map. Main goal of this work is to create an application for estimating object distance from car using methods for depth map estimation. The work also includes description of main steps to achieve goal, like image capturing, stereo camera calibration, image rectification and disparity estimation. The result of this work is an experimental solution for monitoring system used in cars, which focuses on pedestrian detection in front of the car, and calculates its distance by using depth map. Part of this work is also creating test set, which is used for evaluating the accuracy of this system.
|
|
Strategic analysis of the company Adventure World s. r. o.
Armenov, Roman ; Kubíček, Aleš (advisor) ; Machek, Ondřej (referee)
This thesis focuses on strategic analysis of the company Adventure World s. r. o. operating escape games in Prague. The goal of the thesis is to suggest strategic recommendations for further company development. The theoretical part deals with defining basic terminology and describing process of strategy formulation. In the practical part analytical tools described earlier are applied to analysis. In order to analyze external environment, the PEST framework is used. To illustrate the industry environment, 5 competitive forces analysis, map of strategic groups is used. In examining the internal firms conditions, the strategic resources and capabilities are identified through the value chain framework. Analyses findings are summarized in SWOT matrix, then strategy alternatives are generated. In conclusion, the author suggests the wording of the organization mission and updated vision, strategic goals, then author specifies actions, that are meant to fulfill those goals.
|
|
Promotion of selected organisation
Neradová, Renáta ; Švec, Václav (advisor) ; Pavla, Pavla (referee)
The main goal of this thesis is to evaluate marketing promotion in selected organization. Its first part is focused on the analysis of theoretical knowledge in the field of communication, marketing, mainly various marketing communications tools. In the second, practical part is initially introduced under investigation by KOH-I-NOOR Mladá Vožice join stock company, for which are used marketing tools analyzed. Here are described all currently used tools. This thesis is focused on two most important tools for the company. To achieve a partial goal is used evaluation of actively used tools,trades and exhibitions, the method used to describe the passive utilization tool, the website is used the Google Analytics tool. Based on these results are proposed solution for improvement.
|
|
Size-exclusion chromatography - analytická metoda pro charakterizaci rozpuštěných organických látek
PILSOVÁ, Klára
The main aim of this bachelor thesis is the application of size-exclusion chromatography for a measurement of the molar mass of organic matter in water samples from Bohemian Forest lakes and subsequent determination of iron, aluminium and phophorus con-centration by inductively-coupled plasma/mass spectrometry. In the theoretical part, there is information about organic matter in water and the principles of size-exclusion chromatography and ICP/MS. The experimental part deals with the procedure of organic matter measurement and the determination of iron, aluminium and phosphorus concentration. Afterwards, the procedure of SEC and ICP/MS data processing is described and the results are discussed and compared with other analytical techniques.
|
guest ::
