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Semantic differential as a method of detecting positioning and its dependence on contextual conditions
Petrová, Dominika ; Kincl, Tomáš (advisor) ; Novák, Michal (referee)
Thesis deals with measurement of positioning in marketing, specifically the method of semantic differential. The main objective of this work is to verify how it changes the semantic profile of selected wine samples tasted by young wine consumers in changing contextual conditions of this experiment. The experiment consists of two separate measurements, during which there is a change in the contextual conditions. The theoretical part is description of his positioning and measurement, which continuously follows the method of semantic differential, where he presented Seman-tick differential as a tool by which we can measure and record the positioning of his value by creating semantic profiles. The practical part explains the connection and answer research questions determined through experiment, where it was shown that the change in the contextual conditions tasted wine samples young wine consumers, changes the semantic profile of the wine samples.
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Application of Scanning Probe Microscopy for the Study of Ultrathin Films and Nanostructures
Neuman, Jan ; Rezek, Bohuslav (referee) ; Mašláň, Miroslav (referee) ; Šikola, Tomáš (advisor)
Dizertační práce je obecně zaměřena na problematiku mikroskopie atomárních sil (AFM), a to jak vývoje částí těchto mikroskopů, tak i jejich obecnému využití v oblasti výzkumu povrchů, ultratenkých vrstev a nanostruktur. Na Ústavu fyzikálního inženýrství jsou vyvíjena zařízení umožňující aplikovat uvedenou mikroskopickou metodu. V těchto mikroskopech jsou využívány piezoelektrické motory pro zajištění pohybu vzorku a ladicích zrcátek v optickém detekčním systému. Práce se v části věnované vývoji AFM zabývá studiem parametrů řídicích pulzů za účelem optimalizace funkce těchto komponent. Měřením vlivu tvaru pulzů a opakovací frekvence byl jejich pohyb optimalizován z hlediska stability a rychlosti posuvu. V části věnované výzkumu povrchů byly experimentálně zkoumány morfologické změny ultratenkých vrstev zlata na povrchu oxidu křemičitého za zvýšených teplot. Bylo zjištěno, že vhodná povrchová modifikace způsobuje vznik preferenčních trhlin ve vrstvě zlata. Řízeným rozdělením polykrystalické vrstvy na oddělené oblasti je možné významně ovlivnit proces tvorby ostrůvků zlata vznikajících při žíhání. S využitím metod elektronové litografie je možná příprava uspořádaných polí zlatých ostrůvků o velikostech 50 – 400 nm. Dále bylo ukázáno, že zvýšením teploty žíhání na 1000 °C dochází k postupnému zanořování ostrůvků zlata do povrchu. Tento jev je pravděpodobně způsoben přesunem oxidu křemičitého z oblasti pod zlatým ostrůvkem do těsného okolí vzniklého kráteru, kde tvoří tzv. límec. V těchto studiích vedle metody AFM byla s výhodou používána rovněž elektronová mikroskopie (SEM).
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Optimization of the Production of Lipases by Bacillus subtilis
Slavíčková, Radka ; Omelková, Jiřina (referee) ; Hermanová, Soňa (advisor)
In this thesis, optimization of production of lipolytic enzymes by submerzed cultivation of Bacillus subtilis (BS) was studied. Production of lipolytic enzymes was tested in three nutrient media, which differed mainly in main sources of carbon, respectively of nitrogen. The first medium contained mainly extract from calf brain and beef heart (BHIB), the second medium contained peptone and yeast extract (NB) and the third one contained peptone and yeast extract with the addition of 2% (w/v) glucose (NBG). The highest lipolytic activity (0.0784 Uml-1) was measured in NBG medium. Maximum of lipolytic activity was observed before the end of the exponential phase of BS growth in all the media. Temperature optimum in NBG medium was determined from 30 to 50 °C, pH optimum in the range of 5 to 11 and subsequently the temperature stability of lipolytic enzymes produced by the BS was estimated. The activity value was determined spectrometrically using p-nitrophenyllaurate as a substrate. Produced lipolytic enzymes showed maximum activity at 37 °C in the alkaline pH of 8.0. Measurement of temperature stability showed that lipolytic enzymes are relatively thermostable enzymes retaining 100 % of the activity even after 1 hour of cultivation at 30 - 50 °C. The presence of 1% (w/v) olive oil in medium NBG caused a decrease in lipolytic activity by 65 % as well as in pH from 6.5 to 5.4 after 14 days of cultivation. After substitution of glucose by fructose in medium NBG, lipolytic activity showed comparable values during the first week of cultivation. On the other hand, the decrease of lipolytic activity by 29 % in the medium with fructose was observed after 14 days of cultivation. A procedure for the identification of lipolytic enzymes of BS by peptide massfingerprinting was developed to understand the potential of synthetic polyester - poly(e-caprolactone) as a lipase inductor. Degradation study of commercial polyester poly(e-caprolactone) was carried out by submerged cultivation of Bacillus subtilis in NBG medium at initial pH 7.0 and 30 °C for 14 days. PCL (Mn = 10,000, Mw = 14 000) was studied in the form of films (1.0 x 1.0 cm), which were prepared by melt-pressing, rapid cooling of the melt to 4 °C and evaporation of the solvent from 2 % dichlormethane solution. The evaluation of the films shown occurrence of weight loss (7.8 - 17.0 wt.%) together with the formation of numerous holes and cracks in the sample surface in relation to the method of the films preparation. Lipolytic activity values increased by 9 - 17 % in the degradation media compared to control samples. Densitometric monitoring showed also higher increase in cell mass in the degradation medium compared with control samples. Based on the results obtained, the degradation process induced by BS could be suggested.
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Vliv síření na vznik sloučenin vázajících SO2 ve víně
Holka, Andrej
The thesis focuse on the effect of dose levels of sulfur dioxide in the must on the formation of carbonyl compounds. Futhermore it is concerned with oenological properties of sulfur dioxide, its forms and effects on wine. The thesis corroborates in detail carbonyl compounds bind sulfur dioxide and discusses their origin and various options to affect their quantity. Substances that may partially replace sulfur dioxide and thereby reduce its total quantity in wine are also debated. Experimental part of the thesis involved samples of wine differing in quantity of sulfur dioxide added to the must before fermentation and in forms of nutrition. Using the HPLC analysis concentrations of acetaldehyde, pyruvic acid, and 2-oxoglutaric acid were determined. The experiment demonstrated the effect of initial doses of sulfur dioxide on the amount of generated acetaldehyde and pyruvic acid. The effect of addition of thiamine, which reduces formation of pyruvic acid, was also shown.
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Family in pathogenesis of drug dependence (comparative case study)
Horáková, Dominika ; Hajný, Martin (referee) ; Kalina, Kamil (advisor)
This bachelor thesis titled "Family in pathogenesis of drug addiction (comparative case study)" is aimed to the key family factors and circumstances in family, which might have had an impact on development of addiction behavior. The thesis consists of theoretical and practical parts (with three case stories), and of discussion. In the theoretic chapters, the topic of family in general as well as family in pathogenesis of addiction is presented by using knowledge in accessible literature. In the practical part, I am trying to point out the key family factors and circumstances in families of selected clients, which might have had an impact on development of addiction behavior. This is given in two steps: (1) analysis of each case story, (2) comparative analysis of all three case stories according to most important characteristics derived from the theoretical part. At the end, conclusions are presented and discussed. There has been shown that no characteristics is relevant for all three case stories, but there are many features common for case stories No.1 and No.2, and for No. 2 and No.3.
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Nutrigenetic and nutrigenomic aspects at selected pathological states
Krupková, Lucie ; Klusoňová, Hana (referee) ; Hronek, Miloslav (advisor)
DIPLOMA THESIS Nutrigenetic and nutrigenomic aspects at selected pathological states. Mentor of Diploma Thesis: PharmDr. Miloslav Hronek, Ph.D. Lucie Krupková, 2010 Abstract The effect of nutrition to human health is undisputable. Certain dietary compounds can influence each of us in different ways. Although people have almost identical genome, there are imperceptible diffenrences that make us unique. This uniqueness is shown in different responses to specific nutrients. The matter of diet, genes and gen-diet interactions are solved by two developing scientific disciplines - nutrigenomics and nutrigenetics. Nutrigenomics aims to determine the influence of common dietary ingredients on the genome, and attempts to relate the resulting different phenotypes to differences in the cellular and genetic response of the biological system. Nutrigenetics identifies and characterizes gene variants associated with differential responses to nutrients, and relates these variants to disease states. Scientific research is focused primarily on chronic diseases including obesity, cardiovascular diseases, diabetes mellitus type 2 and cancer. The onset of the diseases is caused by enviromental factors on the one hand, and by individual's genotype on the other hand. Nutrigenetics searches for candidates' genes whose...
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Human factor in the aircraft operations
Dostál, Tomáš ; Raudenský, Miroslav (referee) ; Daněk, Vladimír (advisor)
This bachelor´s thesis examines air accidents which is caused by human error. From selected air accidents are pointed out mistakes, which is caused by flight crew, their continuation, increase, main causation and formation air accident. Study of human mistakes and influence phase of flight are shown in diagrams. The conclusion of the bachelor thesis includes possible solving to elimination human error and increasing air traffic safety.
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History and Development of Czech Bibliology and Bibliology Research
Mach, David ; Voit, Petr (advisor) ; Šípek, Richard (referee)
This work deals with the history of Czech bibliology. It follows the development of this branch from humanistic period to Czechoslovak First Republic. It represents famous personalities and their publications or professional activities in bibliology research. There are also shown all ways to define the theory of bibliology and denoted related scientific disciplines. Finally author tries to compare our situation with international movement and trends. The text of the work is divided into several chapters and contains various notes with citations. The description of earlier periods (15-19th century) includes for example humanistic panegyric poetry dedicated to typography, the legend about Gutenberg's Czech origin, Prague baroque celebration reminiscing about the discovery of book printing and especially bibliographical works of Karel Rafael Ungar, Josef Dobrovský, Josef Jungmann or Václav Hanka. Moravian researchers (Cerroni, Dudík) are mentioned separately. Next are analysed relevant works of Antonín Rybička, Zikmund Winter or Čeňek Zíbrt. The large part of text is occupied by detailed characteristic of our interwar bibliology, which was formed by Zdeněk Václav Tobolka, Josef Volf, Cyril Antonín Straka, Vít Hůlka, Anna Císařová-Kolářová or Karel Kabát. The contemporary librarian educational system and...
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Study of nucleic acids by means of drop coating deposition Raman microspectroscopy (DCDR)
Souček, Pavel ; Kopecký, Vladimír (advisor) ; Mojzeš, Peter (referee)
The work was focused on possibilities of the new technique of nonresonance Raman spectroscopy - drop coating deposition Raman (DCDR) spectroscopy upon study of nucleic acids. DCDR spectroscopy is based on deposition of a small droplet of the studied sample on a hydrophobic surface, where after evaporation of the solvent, ring of the studied material in glass phase is formed. That way the material is concentrated and upon measurements by means of Raman microspectrometry the increase of signal of several orders of magnitude is observed with respect to the standard technique of sample measurements in solvent. In this work we studied behavior of DNA 12-mer and DNA with 3000 base pairs. Our research showed that DCDR spectra of DNA dissolved in deionized water can be measured up to concentration of 30 M per base. The DCDR spectra are similar to those measured from solvents. Nevertheless, the deposited sample never forms a ring. It was shown that the size of nucleic acids didn't play dominant role upon ring formation but their charge which lead to repulsion of the molecules. After adding of sodium ions and subsequently also magnesium ions the ring formation was observed. Factor analysis of spectral maps demonstrated that formed rings are not fully homogenous and that upon their origin condensation of DNA could...
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The Thangkas - Tibetian Paintings with a Story
Gyaltso, Lenka ; Czumalo, Vladimír (advisor) ; Bělka, Luboš (referee)
Thangkas are Tibetan paintings which can be explained in many different points of view. Their meaning is different for Buddhist practitioners, painters, collecters, arts historians or researchers. This thesis should introduce the variaty of perspectives. The composition of the painting is given by the patron, artist or follows the Buddhist sacred scripts. Preparing the base, drawing, using colour pigments, outlining and doing final details belong to the process done by the master himself or partially by his students or monk or layman helpers. For impowering the thangka for the Buddhist praxis is necessary to do a sacrification ceremony by an educated monk. It is a religious and also social event connected with the painting. Thangkas are then used for the visualisation of figures of the Tibetan pantheon, mostly peaceful and wrathful deities. They are used in the monasteries, temples, home shrines or altars, are part of Buddhist ceremonies and festivals shown to the audience hung on the terraces or carried by the monks. Their vivid topics and colours encharme not only monks and lamas but also laymen. The expression differs according to local schools influenced by artists of Kashimiri, Bengali, Nepalese or Chinese origin. Tibetan painting style was probably created in the second half of 15th century...
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