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The optimization and validation of HPLC method for determination of triamcinolone acetonide in topical pharmaceutical product
Krivda, Anton ; Matysová, Ludmila (advisor) ; Nováková, Lucie (referee)
Author: Krivda A. Title: The optimalisation and validation of HPLC method for determination of triamcinolone acetonide in topical pharmaceutical product Language: Czech Keywords: HPLC Triamcinolone acetonide Triamcinolone Methylparaben Propylparaben Validation A novel reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone and two preservatives presented in the cream - methylparaben and propylparaben, using estradiole as an internal standard. The chromatographic separation was performed on a Supelco Discovery HSF5 column. The mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (45:55 v/v). The analysis time was less than 9 minutes, at a flow rate of 0,6 ml/min and detection at 240 nm. The method was found to be appicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolone cream 0,1%.
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HPLC determination of betamethasone and chloramphenicol using alternative stationary phases
Salabová, Markéta ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
A new procedure has been developed for separation and subsequent determination of betamethasone and chloramphenicol in pharmaceutical eye drop BETABIOPTAL ® preparation. The analysis carried out using a mobile phase of acetonitrile / water (25:70, v / v), adjusted to a flow rate of 0.75 ml / min at a temperature of 30 ř C, sample injection 5 Ll in the isocratic mode and the detection wavelength of 241 nm. Internal standard with similar properties as the two active substances has been selected as propylparaben. There was chosen for the separation column Synergi Fusion RP MS 2 cm × 2 mm, with a particle size of 2 m. The peaks have sufficient resolution and symmetrical shape and one analysis lasted up to 2 min while maintaining the quality of measurements (accuracy and reliability). The optimized method was validated before the actual measurement.
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Measurement rainfall kinetic energy using laser disdrometer
Jelínková, Petra ; Petrů, Jan (advisor) ; Hrabalíková, Michaela (referee)
The issue of water erosion is spread all over the world. It is closely related with soil aggregates breaking up and the subsequent soil nutrients loss. The total and intensity of rain are important parameters for determining the kinetic energy of rain. The measurement of rain characteristics was done in the laboratory at Czech University of Life Sciences Prague. To obtain the data rain simulator and laser disdrometer were used. With its help the intensity, volume, spectrum, even size and falling velocity of rain was found out. Overall 90 measurements were taken which describe the simulated rain characteristics in detail. Each measurement took 5 minutes. The kinetic energy was found out from the size of raindrop and its falling velocity. The average intensity of rain was 58.6 mm/h and reached KEtime 706,6 J m2/h. The droplets diameter median of all measurements was set in range of 0.375 to 0.5 mm.
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Nanofiber Membranes as Drug Carriers 5.
Beneš, Michal ; Vachek, Josef (referee) ; Doležal, Pavel (advisor)
M. Beneš Abstract The theoretical part of this work deals with chitosan polymer and its derivates. A polymer molecule is described in more detail, its solubility and chemical modifications are described separately. In the next part biocompatibility and biodegradability of chitosan are dealt with. Parameters of the electrospinning of chitosan end this chapter. The experimental part is first dedicated to the determination of aquosity in the samples of 3 different nanofiber membranes and to their gravimetric changes by keeping in the desiccator. The samples of membranes absorb water at the most up to 1,5% (m/m) and there is not a big difference among them. The second part quantifies changes in the composition of the saturated excipients which were used for the impregnation of the samples of nanomembranes. The highest amount of caffeine was absorbed by the membrane 3, namely in case of both saturating media. The evaluation of the amount of caffeine which the tested membranes are able to release from their surface in vitro follows. In case of the saturation of membranes by the aqueous solution of caffeine and by the caffeine suspension is the maximum of the subsequent liberation into the acceptor phase reached from the 120th minute. In case of the ethanolic and chloroform saturated solution is the maximal...
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Determination of Nefopam by the Technique of Sequential Injection Analysis with Chemiluminescence Detection
Štěpánková, Lucie ; Pospíšilová, Marie (referee) ; Polášek, Miroslav (advisor)
1.1 Abstrakt A simple and rapid automated method for the assay of nefopam hydrochloride by sequential injection analysis (SIA) with chemiluminescence detection was devised and optimised. The optimised parameters were aspirated volumes of the nefopam hydrochloride sample and reagents, flow rate, composition of the carrier stream, the photomultiplier voltage and the influence of materials used in the production of tablets. Optimum sample volume was 60 μl and the optimum reagent concentrations (and aspirated volumes) were - 10 mM Ce(IV) ammonium sulphate in 50 mM sulphuric acid (40 μl), 3 mM tris(2,2'-bipyridyl)dichlororuthenium(II)hexahydrate in 50 mM sulphuric acid (59 μl) and 7 mM sodium acetate (34 μl). The optimum flow rate in the system was 64 μl/s and that for transporting the luminescent zone to the detector was 78 μl/s. The photomultiplier voltage was 280 V. The carrier stream was ultrapure water. The calibration curve was rectilinear in the range 5.10-7 - 1.10-5 µmol/l of nefopam hydrochloride. The parameters of the calibration curve y = kx + q (where y is the intensity of chemiluminescence in nA and x stands for the concentration of nefopam hydrochloride in µmol/l) were calculated by the linear regression method. The slope k was 22.398 and the intercept q was 0.7161. The correlation coefficient was...
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Creating sightseeing tour to the selected destination
Čambálová, Lucie ; Petrů, Zdenka (advisor) ; Herget, Jan (referee)
The aim of this bachelor thesis was to create innovative, cost effective sight-seeing tour, where active learning about Thailand is daily alternated by rest at sea. Destinations are selected in an unusual combination, which currently are not offered by any travel agency. The tour is also characterized by an appropriate combination of air, road and water transport and interesting excursions, which are included in the price.Bachelor thesis is divided into two main chapters. The first chapter is devoted to the fundamental concepts that need to be well understood for the subsequent orientation in this thesis. The second chapter is dealing with the creation of the sightseeing tour to Thailand. In conclusion of this bachelor thesis it is considered if a target have been met and attachments make up the chapters of photos, spreadsheets and other important information.
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HPLC application in pharmaceutical analysis
Bajcurová, Lucie ; Sklenářová, Hana (referee) ; Matysová, Ludmila (advisor)
The HPLC method for determination of ketoprofen in therapeutic preparations "PRONTOFLEX" - a 10% skin spray and "Ketonal" - a 5% cream has been developed. This method was based on already developed and validated method for analyzing the preparation "KETOPROFEN" - a 2.5% gel. Both of the methods have been developed in the same department. The chromatographic separation was performed on SUPELCO Discovery C18 column (125 mm x 4.6 mm, 5 µm). The mobile phase consisted of a mixture of acetonitrile, water and a phosphate buffer pH 3.5 (40:58:2, v/v/v). At a mobile phase flow rate of 1.0 ml/min, injection volume of 10 μl and UV detection at a wavelength of 233 nm, the total time of analysis was less than 10 minutes. Ethylparaben was used as an internal standard.
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Determination of vitamine C and dehydroascorbic acid using UHPLC-MS method
Proroková, Zuzana ; Chocholoušová Havlíková, Lucie (referee) ; Nováková, Lucie (advisor)
A coupling of Ultra High Performance liquid chromatography with mass spectrometry provides a technique, which is rapid and sensitive. This thesis is focused on the use of UHPLC-MS for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHA). AA is a small polar molecule that acts as an antioxidant. After oxidation AA creates DHA. AA/DHA ratio is an indicator of a redox state of organism. For the determination of AA and DHA several methods have been developed, which usually do not allow the simultaneous analysis, but require multistep subtraction procedure. The optimization of UHPLC-MS method for the determination of AA and DHA include the choice of mobile and stationary phase and mass spectrometry detector set- up. The choice of appropriate conditions depended mainly on retention time and the detector response. The effect of stationary phase, concentration, pH, composition of mobile phase on retention of AA and DHA was observed. Effect of mobile phase on stability of AA and DHA was observed as well. BEH Shield RP C18, BEH HILIC and BEH Amide column were compared. The best results were achieved on column BEH Shield RP C18. Measurements with 0.1%, 0.05%, 0.01% formic acid, 0.1%, 0.05%, 0.01% acetic acid, ammonium formate at pH 3.5 and amonium acetate at pH 4.4 and 6.8 as a water...
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Engine Block Design of Aircraft Powertrain
Kuzník, Bořek ; Drápal, Lubomír (referee) ; Novotný, Pavel (advisor)
This thesis describes the problems of the engine block and crankcase. There is solution of structural design and contains the engineering design of invert six-cylinder engine block with water cooling. Thesis deals with connected equipment and drives. Engineering design is verified by finite elements analysis for the determination of strenght and durability. The ending of thesis analyzes the production methods of designed engine block.
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High performance capillary electrophoresis separation of low molecular weight compounds in biological and enviromental samples
Oanh, Doan Thi Kieu
This dissertation thesis deals with the separation of small ions in biological and environmental samples using capillary zone electrophoresis (CZE) with capacitively coupled contactless conductivity detection (C4D). Electrodialysis (ED) was used for rapid pretreatment of inorganic cations in biological samples that contain large amounts of high molecular weight compounds that can be adsorbed on the capillary wall. In mikroED system the volume of sample needed was as low as 1 mikroL per analytical run. Using the developed system, the analysis of a single drop of whole blood was possible. Open tubular (OT) ion exchange precolumns were for the first time used for in-line sample clean-up and direct injection of biological samples into CZE. The in-line sample pretreatment method was demonstrated on determination of small inorganic cations in blood serum and blood plasma samples. Electromembrane extraction (EME) and CZE-C4D was applied to rapid and sensitive determination of perchlorate in various samples of drinking water and environmental samples.
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