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The drawing up of purpose Map
Adamcová, Jana ; Ježek, Jiří (referee) ; Fišer, Zdeněk (advisor)
The purpose of this bachelor’s thesis is to focus on planimetry and altimetry of the area SEKO Aerospace a.s. in Louny. Then furter creating of the thematic map in scale 1:500, which contains planimetry, altimetry, surface signs of utilities and more detailed planimetric elements of the locality. The resulting map will be used for the service purposes, planning and projection of the structural modifications at the company premises. The theoretical section of work describes all the stages of creating thematic map.
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Auxiliary brake of escalator
Lacheta, Lukáš ; OTIS, Štefan Kapina, (referee) ; Špička, Jiří (advisor)
Purpose of engaged task is elaborate a functional mechanism to emergency brake for escalators company Otis. To this purpose is gradually reached up in several phases. In primary phase was taken view on similar products from competitive company and do not get in conflict with patent right, in second phase was needed delimitate working space for brake and exact specification of straining. In next steps the proposal and test phase is blending together. In this moment emergency brake has own shape and first tests are showing the functions, but as well necessity modification of same elements on brake and on escalator.
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Modification of Physico-Chemical Properties of Biologically Active Compounds
Oktábec, Zbyněk ; Vinšová, Jarmila (advisor) ; Matějka, Pavel (referee) ; Kratochvíl, Bohumil (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Inorganic and Organic Chemistry Candidate PharmDr. Zbyněk Oktábec Supervisor prof. RNDr. Jarmila Vinšová, CSc. Title of Thesis Modification of Physico-Chemical Properties of Biologically Active Compounds The majority of newly developed biologically active compounds (API) belong to the 2. - 4. BCS class, i.e. groups that have problematic permeability and/or solubility. This work deals with modifications of the physico-chemical properties of model biologically active substances. The first group selected for these modifications were bisphosphonates - ibandronate and risedronate. By using a rational selection of co-crystallisation partners, co-crystals of model APIs were prepared with co-crystallisation partners from the group of monosaccharides and their derivatives. Available solid state analytical methods - NIR, Raman spectroscopy, 31 P CP/MAS NMR - were used for the characterization of the resulting cocrystal(s), and in the case of co-crystals of risedronate salt, 13 C CP/MAS NMR, XRPD and DSC were used. There was only one positive sample among all the prepared samples of co-crystals of ibandronate salt, and that was with the use of phenyl-β-D- galactopyranoside as a co-crystallisation partner. PAMPA experiments were...
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Noun phrase complexity in academic written English
Kratochvílová, Pavla ; Malá, Markéta (advisor) ; Dušková, Libuše (referee)
The diploma thesis analyses written academic text. Academic prose is frequently characterized as a highly complex style which is structurally elaborated, contains a large number of subordinate clauses and expresses meaning relations explicitly. However, new research (e.g. Biber & Gray, 2010) shows that complexity of academic writing occurs on the level of noun phrases which often contain extensive premodification and/or postmodification. The thesis studies noun phrase structure in research articles from two disciplines: medicine and sociology. Two articles from each discipline were selected, each yielding 50 complex noun phrases. These 200 examples were analysed with respect to their modification, its form and levels of embedding. The results were compared for both disciplines. The aim of the thesis was to describe complex noun phrase structure and identify its relation to the type of academic discipline. Key words: noun phrase, modification, academic text, sociology, medicine
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Development and validation of HPLC method for the determination of ketoprofen in suppositories
Zahálka, Lukáš ; Matysová, Ludmila (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Lukáš Zahálka Supervisor: PharmDr. Ludmila Matysová, Ph.D. Title of Diploma Thesis: Development and validation of HPLC method for the determination of ketoprofen in suppositories The purposes of diploma thesis were development and validation of HPLC method for the determination of ketoprofen in Ketonal® 100 mg suppositories. Ketoprofen belongs to NSAID and it is used for symptomatic therapy of inflammatory, degenerative and metabolic rheumatic diseases and for palliative therapy of some urgent or chronic painful syndromes. During development of determination of ketoprofen in suppositories were used two methods, which were developed at the Faculty of Pharmacy in Hradec Králové. Method for determination of diclofenac in suppositories was default for sample preparation and method of HPLC determination of ketoprofen, methylparaben and propylparaben in gel was basic for conditions of HPLC analysis. Development of method consisted in modification of origin sample preparation method; chromatographic conditions have not been changed. During the experimental work was found to determine what influence has the various steps of sample preparation and extraction temperature. The time of extraction...
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Dictionary methods as second phase of BWT
Kovalčin, Stanislav ; Žemlička, Michal (referee) ; Lánský, Jan (advisor)
Burrows-Wheeler transform is one of the most favorite lossless data compression algorithm. Second phase of Burrows-Wheeler transform consists of combination of Move-tofront, Run-length encoding algorithm and used to be written by Huffman or arithmetic encoding. Dictionary methods are used by means of LZ family algorithm in another lossless data compression algorithm group. This master thesis is experimentally testing suitability of integration selected dictionary methods (LZC, LZSS) in second phase of Burrows-Wheeler transform, not only over alphabet of symbols and words, but also over alphabet of syllables. This suitability is tested likewise on large XML files. It is appropriate to propose modification of Burrows-Wheeler second phase's algorithms for large alphabets. Comparation of compression ratios not only over large XML files, but also over Calgary corpus with others programs using Burrows-Wheeler transform is presented.
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Characterisation and chemical modification of halloysites
Vašutová, Vlasta ; Pospíšil, Miroslav (referee) ; Hradil, David (advisor)
Twelve halloysites from different sources in Slovakia, Turkey, China, New Zealand and U.S.A. have been characterized by combination of analytical methods together with the commercial sample of halloysite supplied by Sigma-Aldrich. The aim of this work was to select suitable candidates for to be used as carriers of porphyrine photoactive molecules. In nature, the formation of halloysite is related to the weathering of magmatic rocks or to the hydrothermal alteration of volcanic rocks, frequently in the contact with limestones. Halloysite belong to the kaolinite group, but, contrarily to kaolinite, it contains molecules of water in the interlayer space. It occurs in two forms: hydrated halloysite (10 ?) and dehydrated halloysite (7 ?).Dehydrated halloysites contain more admixtures than hydrated ones ? typically kaolinite, quartz, cristobalite, alunite, gibbsite and in one case also potassium mica. In samples containing both dehydrated halloysite and kaolinite their 001 diffractions overlap. Interaction with formamide was used in these cases to increase the interlayer space of halloysite and thus shift its basal diffraction to lower angles. The basal 001 diffraction of kaolinite after this treatment remains on 7 ?. Silver thiourea method (AgTU) was used to measure the cationic exchange capacity (CEC). Silver...
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Posttranslational modifications and structural alterations of protein synthesis elongation factor Tu in Actinomyces in relation to their life cycle
Holub, Martin
Posttranslational modifications and structural alterations of protein synthesis elongation factor Tu in Actinomyces in relation to their life cycle Protein synthesis elongation factor Tu represents a multifunctional protein with potential role in signaling and regulation of cell metabolism. The complex life cycle of Streptomycetes requires monitoring of changes in their environment and signaling pathways to control it. Here we present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor with the aim to follow developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added Strepomyces aureofaciens EF-Tu in membrane proteomes. We used Mycobacterium smegmatis, fast growing non-pathogenic Mycobacterium, as a non-differentiating actinomycete comparative model. Phosphorylation of intrinsic M. smegmatis and externally added Streptomyces EF-Tu was followed in membrane proteomes from exponential and stationary phase of M. smegmatis liquid culture. We have found that Streptomycetes membrane fraction contains protein kinase(s) catalyzing phosphorylation of both, its own, and an externally added EF-Tu, whereas Mycobacterium membrane fraction contains protein kinase phosphorylating only its own EF-Tu. In vitro phosphorylation...
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Development of stability indicating method for chosen preparation
Chmurová, Klára ; Kastner, Petr (advisor) ; Mokrý, Milan (referee)
1 Abstrakt Development of stability indicating method for chosen preparation(HPLC determination of benzyl alcohol in vaginal cream) Graduation thesis Klára Chmurová Charles University in Prague, Pharmaceutical Faculty in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The task was to find out a method of simultaneous determination of benzylkalcohol and imidazol (decomposition product of main substance of cream - clotrimazole). This thesis is intent on determination of benzylalcohol. We were looking for proper modification of the pharmaceutical preparation (vaginal cream), then optimisation of chromatographic conditions and validation of this HPLC method also. The best results were obtained with LiChrosphere RP 18,5 µm column (125 mm x 4,6 mm, SUPELCO, ), methanol : buffer (solution of 250 ml water, 1,05 ml triethylamine and 0,333g natrium hexasulphonate, pH = 7, set up by 85% H3PO4) 30 : 70 (v/v) was used as the mobile phase, flow rate was 1 ml/min. Detection was realised by UV detector with wave length set up on 223 nm. Time of analysis was shorter than 10 min.
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