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Formation of biofilm by probiotic bacteria and its processing to solid drug form.
Grossová, Marie ; Rittich, Bohuslav (referee) ; Buňka, František (referee) ; Márová, Ivana (advisor)
The aim of present work is cultivation of probiotic bacteria L. acidophilus, B. breve and B. longum in such a way that the culture forms cells clusters or comprehensive biofilm on the variety of free carriers. Biofilm formation of L. acidophilus on the silica from point of view bile and acid tolerance in gastrointestinal tract was studied. While the number of living cells in planktonic form (planktonic form) at pH 1 fell by 30 %, the viability of the biofilm cells was maintained to 90 % under the same environmental conditions. The biofilm culture showed also the protection against environment contained bile. Furthermore, the possibilities of drying procedures of biofilm cultures used as commercial technologies in pharmaceutical industry were studied. The comparison of freeze-drying and fluidization bed drying showed, that freeze-drying is more suitable method, which is able to achieve higher amount of viable cells after drying than fluidization bed drying. The effectivity of freeze-drying method is dependent on the selection of suitable cryprotective medium. In this case, about 90 % higher viability after freeze drying was achieved in comparison with fluidization bed drying. Finally, the industrial processing of probiotic strains into the solid dosage form was studied. Tablets should be produced at hardness between 70 and 90 N and water activity of tablet mixture can be maintained below 0.3. Consequently, the drying step of the tablets in a hermetically closed space with at least 10 % of silica gel must be ensured. Thereafter, the tablets contain (5.4 ± 0.7)109 viable cells after 6 months of drying process. Capsule production technology has no significant effect on the cell‘s viability during production. The triplex blistering foil for primary blistering of probiotic capsules was chosen. The triplex foil, which has low values of water vapour transition rate (0.07 g H2O / (m2 × day) and oxygen transition rate (0.01 cm3/m2 × day), was chosen. Other studied blistering foils commonly used in the pharmaceutical industry are not suitable for long storage of solid dosage forms contained probiotics.
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Discourse analysis of global climate change. Case of emissions - argumentative strategies of actors
Cholt, Jan ; Šmídová, Olga (advisor) ; Hájek, Martin (referee)
Aim of this study is to improve understanding of practical application of public media discourse about Global climate change. Theoretical base is embedded in context of humannature relationship. My premise is that risk of global warming or global climate change is hard to sense by individuals hence dependent on expert knowledge and mediating of these thematic fields toward public, mainly by mass media. Introduced topic, or its selected part, was investigated using discourse analysis. It serves as a tool to identify and discuss patterns of thoughts, elements of argumentation and actor's strategies. They are parts - or even base - of media discourse about main topic, objects, subjects and time-space and others contexts. They are systematically coded according to discourse rules in a repeating categories and their overall configuration creates underlying concept of understanding the topic. Data for analysis consist of collection of utterances made in Czech public media discourse during period of December 2004 and June 2005. Powered by TCPDF (www.tcpdf.org)
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Media coverage of the self-immolation of Jan Palach in Rudé právo, Reportér and Zprávy
Kaňková, Kateřina ; Suk, Pavel (referee) ; Končelík, Jakub (advisor)
Diploma thesis Media coverage of the self-immolation of Jan Palach in Rude pravo, Reporter and Zpravy attempts to show how the ruling ideology influences the media coverage of an event by analyzing newspaper articles from three different periodicals (Rude pravo, Reporter, Zpravy). In this case, the event is Jan Palach's protest against the political and social situation in Czechoslovakia after the arrival of the Warsaw Pact troops in August 1968. Palach performed his protest in January 1969 inspired by the form of self-immolation displayed by Buddhist monks in Tibet. It appears that the political situation at that time can be characterized as a transformation from democracy to tatalitarianism. The official newspaper of the Communist Party Rude pravo was neither using censorship nor distorting reality. It captured the events essentially as they had been running, though there were no arguments or comments. The comparison of Rude pravo with the other two newspapers, Reporter, representing pro-reform democratic media, and Zpravy, representing totalitarian media, reveals the fact that articles in Rude pravo were neutral.
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The company in a competitive environment
Dvořáková, Lenka ; Šrédl, Karel (advisor) ; Hana, Hana (referee)
The Master´s thesis is dealing with characteristic of a firm in competitive environment. The theoretical part describes various forms of markets, both perfect and imperfect competition. Furthermore, in this part will be characterized the concept of marketing mix, internal and external environment of a company, and also selected analyses from both these environments. The main part of the thesis is focused on three media agencies, which will be described in detail. Also, locations where these agencies operate will be described. Locations determine positions of these firms in the market and their respective competitive powers. In the analysis section, I will apply the Porter Five Forces model and the SWOT analysis on these selected firms. Subsequently, I will be dealing with advertisement since it is a crucial part of the marketing mix, very important for comparison of the agencies. The practical part will present a research and a questionnaire. The research is focused on the influence of advertisement on public. The questionnaire is dealing with cultural events. The thesis compares total revenues, analyses results of the investigation, and proposes ways that the selected agencies could achieve better position in the market.
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Psychosocial Help to Victims of Mass Accidents in the Czech Republic
Melková, Ludmila ; Vojtíšek, Zdeněk (referee) ; Mašát, Vladimír (advisor)
Psychosocial help to victims of mass accidents is quite a new term in the Czech Republic. Al though there exist subjects which are capable of offering such help, still, those are rather exceptions. The objective of this thesis was to explain what psychosocial help is about, where the emphases lie and to summarize selected pieces of experience with helping the victims of mass accidents within the Czech Republic and describe and offer principles which would lead to improvements in the forms of this help.
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Role of mass media in shaping the collective memory in contemporary Japan
Kovácsová, Petra ; Sýkora, Jan (advisor) ; Labus, David (referee)
The thesis is focused on Japanese media and especially on their role in the Japanese society. Their impact is noticeable in the way of shaping the national collective memory. One of the unsolved issues of Japan's past, which still resonates in the Japanese society, is the issue of the former comfort women. From the beginning of the 1990s the thus far neglected issue has been given more media attention, which makes it an ideal study case for the influence of media on shaping the collective memory of a nation. With the help of one media that has been following the plight of the former comfort women incessantly till now, the Asahi Shinbun, the thesis documents the method, by which the issue has been dealt with, and the response of the public to the articles of this daily newspaper, which it has created. As the topic has been being discussed for over thirty years, the analysis of the articles is concentrated on two key periods: 1991-1997 and 2010-2014. Using Susan Pharr's model of the types of roles of mass media, the thesis seeks to learn what role the Asahi Shinbun plays in this issue and whether anything has changed in the last thirty years. Key words: role of mass media, comfort women, collective memory, public opinion, Asahi Shinbun
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Masculine and feminine gender in Italian: not only grammatical category
Luhanová, Eliška ; Špaček, Jiří (advisor) ; Štichauer, Pavel (referee)
Key words: grammatical gender, nouns, language sexism, society, lingustics This work focuses on phenomenon of nomi di azione which indicate professions, occupations or titles in Italian. In this limited group of substantives exists inequity between used masculines and feminines. The crucial source of this work is an important work of Alma Sabatini Raccomandazioni per un uso non sessista della lingua italiana (1986), which consists of recommendations for more appropriate and neutral expressions. The work managed to include this topic into the public consciousness and point out to its impact for society. Practical part of the work is based on analysis of competing forms of words, which do not fit common language standards or social conventions. These forms will be examined by five italian expository dictionaries (published between 1998 and 2007) and the Italian corpus of La Repubblica. The results attempt to map current situation and confront it with the recommendations of A. Sabatini. However, the issue is beyond the linguistics area, so the concern of this work is focused also on the socio-cultural and psychological aspects, which condition the whole situation. Among those, the relative disciplines to linguistics are mentioned - politics, mass- media and education. This work does not present clear...
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Digital comic
Zajíčková, Žofie ; PIXA, Libor (advisor) ; KLIMT, Aurel (referee)
The aim of this work is to give some insight into the enviroment of digital comic and look at what kind of language is this new medium using, what is it´s specific quality. The work should put some light on what we can imagine when we say „Digital comic“, scatch out some possible issues around comics that had been transferd into digital form after they were released like a classical comicbooks. After that I am trying to analyse few works that had been designed specificly for the digital enviroment. At the end I am comparing these works to the classical comicbooks and trying to find out what makes them specific. I´m also finding an answer to the question what are the basis of these digital comics that didn´t lost characteristics of their original comic medium and at the same time were able to absorbed some new media.
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Dynamics and spatial extension of recent landslides at the foot of Krušné Hory Mts.
Burda, Jan ; Vilímek, Vít (advisor) ; Kirchner, Karel (referee) ; Šilhán, Karel (referee)
Dynamika a rozšíření recentních svahových pohybů na úpatí Krušných hor | Abstrackt 1 ABSTRACT Recent mass movements currently comprise one of the main morphogenetic processes in the extensive anthropogenic relief of the foreground of the Krušné Hory Mountains in the Czech Republic. These mass movements result in several types of deep- seated slope failures, depending on the type of movement, lithological, structural, and geotechnical conditions and the water saturation of the landslide material. This thesis presents broad set of various methods of geomorphology, geophysics, engineering geology, dendrogeomorphology and geodesy in an attempt to fully understand the problem of slope failures in the Jezeří area located at the boundary between the Krušné Hory Mountains and the Most Basin (Czech Republic). An interdisciplinary approach has enabled an in-depth review of both the dynamics and development of recent slope failures as well as morphology and structure of slope failures. There are two sectors - Southeast and Southwest - with different types of slope failures. In the Southwest sector, mass movements occur in thick colluvial mantle and weathered Tertiary claystones. The main factors influencing their development include rainfall culminations, groundwater flowing from the valley of Šramnický Brook and...
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Optimalizace reprodukce a kvality gamet u okounovitých ryb
KŘIŠŤAN, Jiří
Pond and intensive aquaculture of percids relies mainly on juveniles supplied by semi-artificial and artificial reproduction. Fish hatcheries do not always provide optimal conditions for percid reproduction. This project was undertaken to optimize reproduction methods of Eurasian perch (Perca fluviatilis L.) and pikeperch (Sander lucioperca L.). Reproduction parameters were compared in farmed and pond-reared perch broodstock without hormone treatment. Spawning was successful without hormone induction at 92.3% and 76.9% in farmed and pond-reared perch, respectively. Significant differences in morphological characteristics (gonadosomatic, hepatosomatic and viscerosomatic indices), fecundity, hatching, and post-spawning mortality were detected. All results, with the exception of spawning success and post-spawning mortality, were superior in pond reared broodstock. Further studies are required to optimize protocols for using better artificial food in farmed perch and decreased stress and post-spawning mortalities in pond reared perch broodstock. The possibilities of using clove oil, Propiscin, or 2-phenoxyethanol as an alternative to tricaine methane sulphonate (MS222) as anaesthetic, particularly with respect to reducing fish stress, were investigated. The haematological and biochemical profiles of pikeperch anaesthetized with clove oil (33 mg l?1), Propiscin (1.5 ml l?1), 2-phenoxyethanol (0.3 ml l?1), and MS222 (150 mg l?1) were determined. Each tested group was divided into two subgroups; the first subgroup was sampled 10 min after application and the second 24 h post-application. Clove oil was shown to be associated with the lowest effects in pikeperch, and therefore could be recommended as an alternative to MS222, while Propiscin and 2-phenoxyethanol were shown to be unsuitable for use with pikeperch. In Eurasian perch the use of semi-artificial reproduction for mass production of embryos was shown to be simpler and more effective. With mass incubation, 1 134 000 embryos (59% of total production) were obtained with a semi-artificial spawning regime and 796 000 embryos (41% of total production) with artificial spawning. Based on these results, we can recommend the semi-artificial spawning for production of perch embryos. Differing doses of two commonly used hormone preparations: human chorionic gonadotropin (hCG) and mammalian GnRHa (Supergestran) were tested for induction of ovulation in pikeperch. A broad range of effective doses is used in aquaculture of pikeperch. The minimal effective dose based on ovulation success is still missing. Hormone therapy applied to excess can result a detrimental impacts on broodstock fecundity and quality of eggs. Results indicated that a single injection of hCG or Supergestran could be used for successful ovulation, while no spawning was observed in a control group treated by saline solution. Among hormone treated groups, the best results were observed in groups treated with hCG at 500 and 750 IU kg-1 BW and in groups treated with mGnRHa at 25 ?g kg-1 BW. The sperm of pikeperch and Eurasian perch show similar morphology and fine structure, with the exception of differences in arrangement of midpiece and centrioles and the presence of a fin-like structure on the flagellum in pikeperch. Our results suggest use of an activation medium containing Ca2+ for activation of short-term stored sperm. Efficacy of five concentrations of Alcalase enzyme for elimination of egg stickiness compared to a milk/talc suspension was determined. Results indicated that 0.5-2.0 ml l-1 of Alcalase for 2 min may increase pikeperch egg hatching success (80-85%) compared to the milk-talc suspension. The highest hatching rate (85.4%) was observed with 1.5 ml l-1 of Alcalase. The application of Alcalase successfully eliminated pikeperch egg stickiness in 2 min compared to 60 min with traditional milk/clay/talc methods.
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