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Monitoring of Occurence of Endangered and Protected Fish Species in Upper Blanice River basin
HANÁK, Radek
Fish community of the upper Blanice river basin was studied in eight localities between 62 and 77 river´s km. During the research in the year 2004-2006 was evidenced the occurrence of European crayfish (Astacus astacus) and the freshwater pearl musel (Margaritifera margaritifera). By the ichthyological research was found the occurence of thirteen species of fish and lampreys. In the public notice No. 395/1992 Sb. include Lampetra planeri into critically endangered and three endangered species (Lota lota, Phoxinus phoxinus, Cottus gobio). There are occured the allochtonous fish species Salvelinus fontinalis and Oncorhychus mykiss. The dominant kind is Salmo trutta morpha fario. The next species are Leuciscus cephalus, Leuciscus leuciscus, Thymallus thymallus, Barbatula barbatula. Evidently the allochthonous origin are Rutilus rutilus and Perca fluviatilis in this river basin. For the conservation and the biodivesity safeguarding is necessary to keep the extensive land management in this area and the sensibile catchment and do not affected the natural succesion of the watershed configuration by the adverse ameliorative effects.

Influence of meadow management on the migration behavior of selected species of butterflies
Bubová, Terezie ; Langrová, Iva (advisor) ; Farkač, Jan (referee)
Migrations are the key process and one of the basic biological features, which allow survival of endangered species in fragmented landscape. In the last decades, agriculture intensification, industry and urbanization caused that number of natural butterfly habitats has significantly decreased. The localities originally suitable for butterflies are currnetly converted to farmland or building plots. This PhD thesis aimed to find a compromise solution of meadow management, which should allow a commercial use and concurrently do not jeopardize the continued existence of endangered or threatened species. The main idea of this study was in the intentional emigration causing in the middle of flight period, which should lead into resettlement of the population to another (potentially more suitable) locality. Possibility of aimed manipulation with butterfly emigrations would be considered as a major breakthrough in their protection. The investigated species Phengaris nausithous (Bergsträsser, 1779) and Phengaris teleius (Bergsträsser, 1779) are both considered to be flagship species for protection of European butterflies at open meadows. This study is based on research, which was carried out in 2013-2015 at 17 patch in localities Dolní Labe, Czech Republic. In above mentioned years, one selected meadow was mowed in the middle of flight season and the emigrations were then monitored using mark-release-recapture method. Results of population and migration characteristics were calculated in MARK 8.1 software. Subsequently, the effect of mowing on emigration (comparison of emigrations from the meadow before and after mowing) was evaluated using Statistica 13. The hypotheses of this study were based on the assumption, that the loss of resources after well-timed mowing should increase the activity of adult butterflies. During the monitoring, number of emigrations was increased only in flight season 2015. Unfortunately, the statistical evaluation did not show significant effect of mowing on migrations. On the other hand, the hypothesis dealing with colonization of nearest meadows was confirmed. Effect of intentional emigration caused by management interventions was unfortunately not proved. However, we found out, that even though the population at one mowed meadow decreased clearly, the metapopulation size at the locality remained more or less stable. At investigated (mowed) meadow, there was observed a sharp decline of P. nausithous abundance. On the other hand, P. teleius population was only slightly reduced. It is therefore conceivable, that P. teleius are not as susceptible to innapropriate term of mowing. The results obtained in our research conclusively displayed no possible positive effect of mowing in the middle of flight season and a necessity of suitable management maintenance at localities inhabited by Phengaris butterflies.

Non-invasive methods for sex and steroid homones determination in gibbons of the genus Nomascus
Bolechová, Petra ; Bartoš, Luděk (advisor) ; Pavel, Pavel (referee)
The gibbon primates of the family Nomascus are classified as critically endangered species, and, to date, basic understanding and information about their biology is missing. With regard to the status of these animals in the wild and captive populations in zoos, being familiar with their reproduction, may improve captive breeding programs. Data collection in this study was to be carried out by the practical use of non-invasive methods (polymerase chain reaction for DNA extraction and enzyme immunoassays), using faecal samples for analysis. The first method, polymerase chain reaction, was used for sex determination in juveniles, because of their coat colour and visual similarity of secondary sex characteristic in both sexes; it is not possible to determinate sex without handling the animal. Another main purpose of this study is to try and answer the hypothesis regarding the ovarian cycle of females, factors influencing their hormone concentration and also the onset of sexual maturity in females and the timing of their fur colour change. Hypotheses were checked by monitoring the concentration of progesterone and oestrogen faecal metabolites and by evaluation of the composition of breeding groups of gibbons with the ZOO influence. During a four year period (from 2010 till 2014), there were a total of 51 animals analysed from 16 different zoos with a faecal sample count totalling 1618 samples. The results confirmed the use of noninvasive methods for sex determination, and thus ensuring the maximum welfare standards. Endocrinological analysis confirmed the hypothesis of the influence of the environment (ZOO) to the hormone concentrations and female´s pregnancy effect in conjunction with her age on the final results of the male - father hormone concentrations. The initial information in this study is the confirmation of the onset of ovarian cycle in young females without connection to their fur colour change and a significant factor of a mother´s impact and her territoriality. This study is the first to present a long term monitoring of ovarian cycles in females and hormone concentrations of other individuals, both male and female, and from various age groups. The results allow us to understand the possible impact of zoo environments on the reproductive status of these gibbons and contribute to the general improvement of breeding management.

Assessing the Synergistic Effects of the Selected Consolidated Entity
Babáčková, Markéta ; Kubovic, Pavel (referee) ; Rajchlová, Jaroslava (advisor)
The thesis focuses on the methods of assessment of synergies established in a consolidated entity. The text includes a definition of a consolidated unit based on theoretical grounds and a draft set of indicators suitable for the analysis of the achievement of synergies. Synergies are monitored through a comparison between the consolidated unit and the mother company made in several selected areas. Results of the analysis of the draft indicators are assessed to determine whether synergies were achieved.

Závislost kontrastu na tlaku segmentového ionizačního detektoru v environmentální REM
Černoch, P. ; Jirák, Josef ; Autrata, Rudolf
Ionization detector utilizes impact ionization in electrostatic field in gaseous environment in the specimen chamber for signal detection. The contrasts between four metal materials using ionization detector with three concentric electrodes in various connection of the electrodes were monitored. As experimentally determined, the grounding of the outer electrode has a positive influence on the image contrast.

Monitoring of surface changes caused by underground mining in the Karviná region
Doležalová, Hana ; Kajzar, Vlastimil
Geodetic monitoring of the Louky locality revealed irregularities in the development of the subsidence depression and it also helped to document the changing terrain due to ongoing reclamation activities. Repeated GNSS measurements recorded not only the size of subsidence of individual points, but also the size and direction of horizontal displacement of these points, which revealed the influence of undermining in the area of interest, and in its surroundings. It turned out that particularly the significant tectonic fault A forms a barrier of the influence of underground mining. The subsidence depression develops differently than the models of the predicted subsidence expected, especially in the area between the tectonic faults X and A. The method of aerial photogrammetry recorded not only the subsidence caused by underground mining, but also the extent and size of the changes caused by the ongoing reclamation of the surface.
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Proposals to Improving Cost Management in a Selected Company
Klímová, Barbora ; Tučková, Hana (referee) ; Hanušová, Helena (advisor)
The subject of this thesis are the costs of a specific company, their monitoring and ma-nagement. The theoretical part summarizes the information that served as the default resources. These findings have been applied in the practical part, for the selected com-pany. In the final section are formulated suggestions, which should lead to better ma-nagement of the costs.

Radio frequency resonator for feeding ion trap
Jedlička, Petr ; Pham, Minh Tuan ; Čížek, Martin ; Pavelka, Jan ; Lešundák, Adam ; Hucl, Václav ; Hrabina, Jan ; Řeřucha, Šimon ; Lazar, Josef ; Číp, Ondřej
Two versions of resonators were designed and manufactured - asymmetric and symmetric.\nThe resonator consists of a coil and capacity of ion trap which makes parallel resonant circuit.\nEnergy is supplied to the resonator through the coupling inductance. Resonator assembly was equipped with outputs for monitoring high-frequency voltage. Both versions have been tuned and tested.\n\n

Possibilities in monitoring of laser welding process
Horník, Petr ; Mrňa, Libor
With increasing demands on the quality of the welds, it is usual to apply automated machine welding with monitoring of the welding process. The resulting quality of the weld is largely affected by the behavior of keyhole. However, its direct observation during the welding process is practically impossible and it is necessary to use indirect methods. At ISI we develop optical methods of monitoring the process based on analysis of radiation of laser-induced plasma using Fourier and autocorrelation analysis. Observation of keyhole inlet opening is partially possible through a coaxial camera mounted on the welding head and the subsequent image processing. A high-speed rear camera to understand the dynamics of the plasma plume. Through optical spectroscopy of the plume, we can study the excitation of elements in a material. Shielding gas flow can be visualized using schlieren method.

Sledování konformace peptidové vazby u N-metylovaných peptidů pomocí RP-HPLC a NMR
Sýkora, D. ; Žáková, Lenka ; Buděšínský, Miloš
Significant peak broadening was observed for some N-methylated peptides as a consequence of cis/trans isomerism of the peptide bond. Chromatographic behavior of the N-methylated peptides was compared with data obtained by NMR.