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Study of diaphragm pump with linear motor
Dubový, Ľuboš ; Habán, Vladimír (referee) ; Pochylý, František (advisor)
The content of this master‘s thesis is a study of diaphragm pump with linear drive. Linear motor was used as the linear drive. Thesis is well – founded by theoretic calculation of pump and its design draft. Likewise, the thesis includes optimalization of suction, or pressure, valve for pump, created by using computer fluid dynamics software. Thesis is complemented by basic view of different types of pumps based on the classification by hydrodynamic and hydrostatic pumps. Subsequently, the preview of blood – flow pumps is presented.
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Biochemical methods as tool for study of reproductive proteins
Postlerová, Pavla ; Zigo, Michal ; Pohlová, Alžběta ; Jonáková, Věra
Study of molecular mechanisms in reproduction is essential for the understanding of this outstanding process. Our lab studies proteins secreted by reproductive organs and sperm using various biochemical methods for a long time. We have expertise in protein extraction from spermatic cells using different approaches, and by kits for proteins from the sperm surface and distinct subcellular compartments. The proteins of reproductive organ fluids are separated by chromatographic methods, such as size exclusion chromatography, high-performance liquid chromatography with reverse phase (RP-HPLC) and affinity chromatography on matrices with various ligands. Proteins are subjected to SDS- or 2D-electrophoresis for their characterization and comparison of various extraction methods, different mammalian species, and sperm in different functional development. Electrophoretically separated proteins may be transferred onto nitrocellulose membrane (Western blot) for antibody detection or binding studies with lectin-labelled ligands (lectins, polysaccharides, zona pellucida glycoproteins). We use immunoprecipitation method with specific antibody for protein determination followed by the MALDI identification. Proteins are localized by immunofluorescent techniques on/in spermatic cells and tissue sections of reproductive organs. Isolation of proteins from reproductive tissues and fluids, and the antibody detection is crucial for the studying of reproductive protein origin.
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Quantitative structure-antimycobacterial acitivity relationships in the group of potential antituberculotics
Doležal, Rafael ; Waisser, Karel (advisor) ; Lehotay, Jozef (referee) ; Ponec, Robert (referee)
DOLEŽAL Rafael: Quantitative relationships between the structure and antimycobacterial activity of selected potential antituberculotics. Hradec Králové: Faculty of Pharmacy in Hradec Králové, Charles University in Prague, 2008, 274 pp. Ph.D. Thesis. The Ph.D. thesis deals with analysis of quantitative relationships between the structure and antimycobacterial activity (QSAR) of four structural classes of potential antituberculotics - derivatives of N-phenylcarbamic acid esters, derivatives of 1-phenyl-1H-tetrazole-5-thiol, derivatives of salicylamides and derivatives of salicylthioamides. In total, 600 computer models of different compounds were created. 204 of them, after conformational analysis in HyperChem 7.52 and geometric optimization with the B3LYP/6-31G* DFT method in Gaussian 03, provided data for calculation of several types of descriptors. Attention was turned especially to local, quantum-chemical descriptors of electronic properties, such as superdelocalizability, index of frontier electron density and Mulliken electric charge. The QSAR analyses are based on searching for statistically significant correlations between the antimycobacterial activities in vitro and a matrix of various descriptors by means of multiple linear regression (MLR) with the use of a selection algorithm, by the...
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The system of education in the Czech army
Šplíchalová, Vladana ; Vybíhal, Miroslav (referee) ; Mužík, Jaroslav (advisor)
This thesis provides information and clarity on the education system in the army of the Czech Republic, as well as its role and the reason why it is needed. The thesis also describes individual steps of this education system, including a system for life-long education. This document aims to provide basic information regarding the army of the Czech Republic, primarily the need for its existence and its tasks, but also its role in the world-wide context. Furthermore, it looks deeper into high-school and university education, as well as a vast life-long education system. The life-long education system covers an extensive offer from elementary to highly specialized courses. Additionally, the thesis tries to clarify potential career opportunities for soldiers leaving the army. The points stated above, such as the scope of this system, and life-long education, have become the major reasons why I have selected this theme. Last but not least, the thesis provides a constructive view of this education system, some of its challenges and new areas to explore. It provides an overview of all the education activities in the army. This can help those who would like to get to know more facts about this complex system, since the times of army mandatory service are gone, and as such, there is not much information available....
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The electrical discharge machining in production of shaped moulds
Kalenda, Jan ; Prokop, Jaroslav (referee) ; Osička, Karel (advisor)
In this diploma thesis is theoretically written about the production of shaped cavities using electroerosive sinking. The possibilities of usage and the production of electrodes for own usage likewise development of technology, tools and machines for electroerosive machining. The quality of machined surface and surface layer using own process of electroerosive sinking of shaped cavities are as well mentioned in this project.
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Virtual Image of the Czech Sports via Unstructured Data Analysis
Levý, Jan ; Jelínek, Ivan (advisor) ; Profousová, Lenka (referee)
Analysis of unstructured data from social media is an important and constantly growing part of information technologies' application in the marketing context. The aims of this bachelors' thesis include creating a trial platform for the analysis of un-structured data from Facebook, research and application of suitable methods and metrics for the analysis, summarizing the analysis' findings and visualisation of selected metrics by means of a dashboard. The thesis' structure corresponds to the methods used for metting the aims set and compo-ses of seven parts. The first part summarizes motives for social media analysis, deals with the approach to the topic in the contemporary scientific papers and discusses comercial tools that could be used for such analysis. The second part describes gathering of data with a crawler, setting the connection and getting the access rights to the data sources. The structure of downloaded documents and appropriate choice of data sources is also described in this part. The third part adresses the tools used for the analysis, namely Elas-ticsearch and Kibana. The fourth part defines possible questions that may be asked in the context of Czech sports image on Facebook. The fifth part identifies the metrics necessary for the questions assesment and describes corresponding data selection. The sixth part completes the analysis itself and summarizes its findnings. The seventh part describes Ki-bana's dashboard use for effective summarizing of selected metrics. The main bachelors' thesis contribution lies in the illustration of social media analysis pos-sibilities on the example of Czech sportsmen and Czech sports in general on Facebook. A crawler has been used to gather the data for the purpose of the analysis. The other em-ployed tools included Elasticsearch and Kibana, which have enabled the data selection and visualisation. The analytical approach consisted of four parts; definition of analytical que-stions, definition of methods and metrics to find answers to these questions, followed by the analysis itself and the summary of results. The following points belong to the analysis' findnings: identification of sportsman with the highest marketing potential, comparison of analysed sports according to the perceived image by fans and desgination of the pages with most interacting followers.
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Sorption and Stabilization of Metals/Mettalloids by Innovative Synthesized Sorbent Amochar.
Ouředníček, P. ; Trakal, L. ; Komárek, M. ; Pohořelý, Michael
Remediation of contaminated soil which is based on stabilization and immobilization of potential\nhazardous substance by sorption materials has been studied intensively nowadays. Biochar – activated organic carbon belongs to this group of stabilizing agents which can adsorb wide range of contaminants, including metals/metalloids. Surface area of the biochars is quite large in general and functional groups (e.g. COO–) can form chelates or alkaline elements on the surface, which is represented by cation exchange capacity. Altogether with the high pH values (7.00 – 10.00), biochars are quite effective sorbents and can adsorb metals/metalloids from the solution (ground water), especially in acidic soils (in the environments affected by intensive mining activities). Sorption\neffectiveness can be increased (especially for As (V) or Cr (VI) sorption) by modification of biochar by various types of secondary oxides. Innovative sorbent AMOchar (AMO + biochar) has been synthesized currently. The product was prepared by adding of biochar to the reaction solution during amorphous manganese oxide (AMO) synthesis. The AMOchar was formed mainly by Mn-oxalates which had coated surface of the pristine biochar. AMOchar composite was able to remove significantly higher amounts of various metal(loid)s from the solution despite the rather high pH of the material. Sorption effectiveness was high not only in case of Pb (II) sorption (almost 99%), and Cd(II) (51.2%), but also a very high amount of As(V), 91.4%. Additionally, both AMOchar composite was able to reduce Mn leaching. This can avoid potential post-contamination caused by the dissolution of less stable Mn-oxalates as observed in the pure AMO.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_22316111113040 - PDF
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Pervaporation of Methanol Water Mixtures by PERVAPTM Asymmetric Membranes.
Izák, Pavel ; Kačírková, Marie
The report summarizes the results of experimental testing of pervaporative separation of methanol from methanol-water binary mixture at 35°C. For the separation were chosen the Sulzer´s PERVAPTM membranes. The concentration of methanol was 6.5 wt.% in water, and pervaporation was performed in vacuum mode. Membranes displayed separation properties suitable for industrial separation of methanol from water mixtures.
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Cultural potential of Myjava „kopanice“ region with bearing on the town of Myjava
Kubík, Ľubomír ; Malcová, Zuzana (advisor) ; Barbara, Hucková (referee)
The thesis targets the cultural potential of the town of Myjava and the interest of its inhabitants in other than traditional forms of the cultural expression. The theory aims to elucidate the issue of regional and local culture, describing individual elements of a local culture and the differences in its definitions. It continues with a brief description of the demographics, more detailed description of the history of the town from the foundation until the present and enumarates the elements of a local culture characteristic for the town of Myjava. The practical part of the thesis examines the current options for culture in the town, describes recurring contemporary events and formulates an hypothesis proposing the town neglects alternative forms of the culture. The hypothesis is attested by an inquiry among the views of the residents and possible solutions are proposed.
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Special diffractive element based on fractals
Kala, Miroslav ; Bednář, Josef (referee) ; Kotačka, Libor (advisor)
This diploma project is focused on use the fractals in diffractive optical elements. Properties of diffraction patterns of various fractals are investigated. Properties of some simpler non-fractal structures are investigated too. Diffraction patterns are created by computer with program written in Delphi programming language. Potentialities of use the fractals in security optical elements (holograms) and their advantages against the classic non-fractal structures.
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