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Mining limits and their effect on labour market in the region of Most
Pícha, Tomáš ; Kotrusová, Miriam (advisor) ; Geissler, Hana (referee)
This Bachelor Thesis is about the mining limits for brown coal mining at the region of Most and problems that could arise if there was a decrease in mining in the region. Although the issue is discussed more and more often, it cannot be considered as solved. This work should serve as deeper insight into the problem and it is primarily trying to answer the questions about potential social issues that are connected with the mining and its possible ending. One of core parts is an analysis of subjects at institutional level. Its purpose is an evaluation of solutions that were suggested by participating subjects.
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Exocyst subunit AtSEC15b: its role in plant cell morphogenesis and characterization of its Rab interacting partner
Toupalová, Hana ; Žárský, Viktor (advisor) ; Baluška, František (referee) ; Hašek, Jiří (referee)
Organization of endomembrane compartments in all eukaryotic cells is dependent on continuous transport of membrane vesicles. Major part of the core regulators of intracellular membrane transport is represented by small GTPases from the Rab family. Rab GTPases cycle between the GTP-bound "active" and GDP-bound "inactive" forms. In their active form, they are able to interact with specific effectors and perform their functions. Exocyst is an octameric complex involved in regulation of secretion. It functions as an effector of Rab GTPases in yeast and mammals and tethers secretory vesicles to the plasma membrane prior to the actual membrane fusion. Using publicly available expression data, we have identified candidates from Rab GTPase family for the interaction with exocyst subunit AtSEC15b in plants and demonstrated that AtSEC15b specifically interacts with AtRABA4a GTPase. We also showed that, like in yeast and mammals, Arabidopsis Sec15b binds Rab GTPase also probably in GTP-dependent manner, implying that this interaction is well conserved throughout the eukaryotic kingdoms. We also successfully demonstrated the complementation of yeast thermo-sensitive mutant strain, sec15-1. Based on this observation we concluded that AtSEC15b is able to substitute the function of yeast SEC15 and restore the phenotype....
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A post-war policy ofthe Czechoslovak Communist Party from 1945-1946 (from the Košický programme to the parliamentary election in 1946)
Duchoslav, Petr ; Rychlík, Jan (referee) ; Kvaček, Robert (advisor)
This work, in detail, deals with the post-war policy ofthe Czechoslovak communist party in 1945-1946. It is defined on one side by the restoration ofthe party and on the other side by the parliamentary election in 1946. The parliamentary election in 1946 ended the first phase of the party' s tactics, whose aim was to infiltrate the state mechanism and, at the same time, the election built optimal conditions for the party to finally eliminate its political enemies and seize absolute power. I have deliberately chosen this demarcation because it takes a key place in the development of the party' s political tactics, and not a period ending in astate coup ď etat in 1948. This approach would have been possible but it might have ended up growing into a simple description in which all the important and relevant matters of the party' s programme and political strategy were left out. The very core ofthis work is made of a post-war policy analysis of The Czechoslovak Communist Party, its historical development in an overall domestic and intemational context. It particularly focuses on the following crucial issues: 1. a post-war restoration of the party; 2. The party's policy in a period ofThe Provisional National Assembly (TPNA); 3. The 8th convention ofThe Czechoslovak Communist Party; 4. The May 1946 election. This...
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Vegetation changes of urban forests
Kučerová, Tereza ; Hédl, Radim (referee) ; Vojta, Jaroslav (advisor)
Urbanization is one of the most intense and biggest factor of homogenization created by human. Cities produce environment which is important just for one species and it is homo sapiens. Very important is also instability of pumping natural resources instead of preservation natural environment. In consequence of cities expand across the planet, biological homogenization increases because the same urban-adaptable species become increasingly widespread and locally abundant in cities all around the World. Synantropic species adapted to intensely modified built habitats at the urban core are global homogenizers, that we found in cities and urban woodlands worlwide. This species consist of early successional plants and edge animal species as mesopredator mammals and frugivorous birds that can utilize gardens, forest fragmnents and many other habitats available in the suburbs. The intentional and unintentional importation of species adapted to urban habitats often produces local species diversity and abundance that is usually equal to or greater than the surrounding landscape. Human populations inhabit richly cultivated suburban habitats with a relatively high local floral nad faunal diversity without awerness of the global impoverishment caused by urbanization. Because so many urban species are immigrants...
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Depiction of barbarians in early Chinese sources (Western Zhou - Western Han dynasty)
Karlach, Jan ; Liščák, Vladimír (referee) ; Maršálek, Jakub (advisor)
The present paper discusses the evolution of the chinese perception on the surrounding nations during the period of formative process of the chinese civilization, namely from Western Zhou dynasty (c. 1045 - 771 B.C.) to Western Han dynasty, which lead this process to its end. The core of the paper describes the depiction of the barbarians in particular period and texts of the chinese ancient era, stresses the changes of this depiction in the course of time and also follows the factors, which could have influenced this perception. A very important part of the paper is a characteristics of the cultural stereotypes, which can be observed in the depiction of barbarians and which seemed relevant to Chinese for description of barbarian's differences.
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Liability for damage under civil law and under commercial law
Laštovičková, Lola ; Švestka, Jiří (advisor) ; Salač, Josef (referee)
The purpose of my thesis is to compare the conformity and diversity between legal provisions of liability for damages in czech civil and commercial law. The thesis is divided into four chapters and its core lies in Chapter Three. First chapter subdivided into three parts deals with characteristics of liability for damages in general, further contains the location of this liability among other types of liability and the last part concerns with the functions of it. Chapter Two is concentrated on the Principles of European Tort Law. Part One describes the europeisation of tort law, which is mostly connected with spontaneous projects of soft law. In Part Two there is reffered to general conception of the Principles and some particular provisions in detail. I have endeavoured to draw a comparison between almost every field of liability for damages in czech civil and commercial law and these Principles. As I have mentioned the main part of my thesis rests in Chapter Three. The chapter consists of eight parts. At first I have explained the relationship between Czech Civil Code and Commercial Code regarding liability for damages. Legal provisions in both codes are sometimes duplicate, on the other hand in some parts different but often without any relevant reason. Subsequent part highlights the importance...
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Optical transmission applications in industries
Worbis, Vít ; Křepelka, Václav (referee) ; Filka, Miloslav (advisor)
The aim of the semestral work term use of optical transmission in the industry is to find the right equipment for communication in industrial applications, which would lead to better control and management of these applications. My show almost seamless connection between the device, which is controlled by another device such as a computer. The first chapter shows how inside the core optical fiber and reflected beams are then carried around the skylight. The second section lists the differences between the copper and fiber cabling, there is described why metallic line slowly destroyed and replaced him fully optics. In the third chapter, I focused on the optical fiber, what are its characteristics, from which parts are made and what is distributed. In the fourth part shows the optical transmitters for multimode and singlemode optical fibers, which are differences between them, which are produced and with what characteristics, etc. In the fifth chapter is applied to a network of possible involvement in the industrial zone, is designed only as they could theoretically be industrial zone controlled by the machine. In the sixth part of the thesis I have tried to design a laboratory task that consists of those optical converters. It is only a small sample, as used in the industry far more extensive involvement.
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The emotional intentions in the interwar political cartoons (1918-1939)
Svobodová, Eva ; Köpplová, Barbara (referee) ; Sekera, Martin (advisor)
Diploma thesis "The Emotional Intentions in the Interwar Political Cartoons (1918-1939)" deals with the view of selected events and personalities, by which is expected a highly emotional (she had: emotionally heady - laden) public evaluation. This view of political themes is followed in the period October 1918 - December 1919 and May 1938 - March 1939, in the objectively politically dynamic periods and within the field of collective emotions hypertrophic periods. The basic thesis states: Caricatures exude mainly negative emotions (though at the same time - secondarily - may involve in the perception positive emotions too, the overall tone of the caricatures and their persuasive intention is primarily an evaluative negation of the shown). Aim of my thesis is to try - by using semiotic analysis - to identify what emotions could be excited by recipients and to provide the emotional agenda. 1 also mention stereotypes in the representation of individual phenomena, but their representation is not the core part of the work. The working method is an innovation of traditional approaches to the interpretation of the cartoons, their forms and contents. Its aim is to verify productivity of this chosen method for any other use. The primary sources are copies of satirical magazines and newspapers, and secondary...
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Topology and function of the transmembrane domain of colicin U produced by Shigella boydii
Dolejšová, Tereza ; Fišer, Radovan (advisor) ; Krůšek, Jan (referee)
Colicin U is a protein produced by strains of bacterium Shigella boydii. It exhibits antibacterial activity against some bacterial strains Shigella and Escherichia. Based on sequence homology with colicins A, B and N, the colicin U is classified as a pore-forming colicin. Interaction of colicin U with attacked bacteria is ensured by three-step mechanism: 1) First colicin U interacts with surface receptors OmpA, OmpF and core of LPS. 2) Thereafter the colicin is translocated to periplasm through interaction with Tol proteins. 3) Finally colicin U interacts with the inner membrane of the attacked bacteria causing its depolarization. In this thesis I demonstrated pore-forming features of colicin U and further observed characteristics and properties of these pores. Using methods of measuring on black lipid membranes I determined a single channel conductance (19 pS), ion selectivity, the influence of various conditions on the behaviour of the pores. These findings, in many cases, correspond to the findings on other related colicins. Furthermore, I successfully determined the pore diameter of colicin U ( ≈ 0,8 nm). The next section of the thesis focuses on creation of single cysteine mutations of colicin U. Subsequently I produced five mutant variants of colicin U and verified their functionality so that...
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Influence of parameters of blood count in dependence on the amount of blood collected into tubes
MOUDRÁ, Pavla
Haematology is the branch of science engaged in the study of blood and its components - especially blood cells - red blood cells, white blood cells and blood platelets. In the core haematology examination - the blood count - not only their amount is evaluated but also the morphology. When blood taking, the right ratio of blood and anticoagulant must be kept, otherwise changes in blood count may occur. Laboratorians are bound to reject examination of incorrectly taken samples. Since it is difficult to take blood from some patient groups (children, elderly, obese patients, etc.), there is often put a pressure on laboratories from the staff to process also the incorrectly taken samples. The aim of this study is to state the level of impact of the incorrect blood taking, within the meaning of the blood - anticoagulant ratio, on the particular parameters of the blood count. The sample consisted of one hundred patients who were invited by their physician for blood tests to the Synlab Czech., s.r.o. company, U Tří lvů 4, České Budějovice in the period from December 2011 to June 2012. There were healthy and sick patients aged 10-77 years in the sample. All one hundred patients were taken within the same examination two test tubes with anticoagulant K2EDTA made by Becton Dickinson - BD Vacutainer. According to instructions, 2 ml of blood were taken into the first tube. Into the second tube, from the fifty patients only 0.5 ml of blood were taken, from the another fifty patients 3.5 ml. These samples were measured in the laboratory of Synlab Czech, s.r.o. company, U Tří lvů 10, České Budějovice using the Advia 120 analyzer made by Bayern HealthCare LLC, serial number IR28850224, installed in 2011. All 13 parameters issued by Synlab Czech, s.r.o. company on the result sheet as a blood count were analysed. The results from correctly and incorrectly taken samples were compared using a paired t-test. Hypothesis assumed that deviations will occur at both sampling errors for all thirteen blood count parameters at the significance level of 95%. This hypothesis was not confirmed, there were no deviations of statistical significance in all of thirteen parameters in any incorrectly taken samples. In the case of test tube where more blood than required was taken, there were no deviations of statistical significance in any of thirteen parameters. In the case of test tube where less blood than required was taken, there were deviations of statistical significance in six of eight measured parameters. A statistically significant increase occurred in the number of red blood cells (by 0.85%), haemoglobin amount (by 0.97%), haematocrit values (by 2.19%) and MCV (by 1.41%). The decrease occurred in MCHC values (by 1.23%) and platelet count (4.96%). The differential white blood cell count was statistically significantly different only in the monocyte value (reduction of 6.35%). Within examination of samples from tube where more blood than required was taken, there were no statistically significant deviations. Therefore, we may assume that such incorrectly taken samples could be used for blood count examination. When taking less blood than required, statistically significant deviations occur in almost half of the blood count parameters. The platelet reduction of 4.96% is clinically the most important. Nevertheless, we can say that even this value is probably not significant enough to not use the incorrectly taken sample in acute cases. If the conclusions of this study are confirmed by other studies, it could be possible to make health care more effective in cases where deviation is considered to be neither statistically nor clinically significant.
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