|
Identification of antimicrobila peptides in spider venom
Benýšek, Jakub ; Liberda, Jiří (referee) ; Tichá, Marie (advisor)
Still increasing resistance to antibiotics leads to the need to find new active compounds with antimicrobial properties. This work is focused on the occurrence, chemical and physical description, mechanism of action and biological activity of such substances, found in spider venom. The second part is focused on isolation and identification of compounds with these properties from the venom of wild bees and a one spider. A novel peptide was isolated and identified from venom of bee Trachusa byssina. This novel peptide possess antimicrobial properties and low hemolytic activity. Molecular weight was estimated to 1749,9 ? 0,1contains 16 amino acids and is amidated on its C-terminus. Its primary structure GILSVLKNLLKKHMAS-NH2 was determined by using Edman degradation and ESI-QTOF mass spektrometry.
|
|
SNP polymorphisms of Y chromosome in the population of african fulani people
Bučková, Jana ; Šimková, Halina (referee) ; Černý, Viktor (advisor)
Markers on the non-recombining region of chromosome Y is a useful tool for study of diversity between populations. SNPs are the most commom polymorphisms in human genome. Mutation rate of SNPs is very low and so they may be used as genetic markers in evolutionary and population studies. We have analyzed 205 unrelated men from 11 Sub-Saharan Fulani's subpopulations. Fulani are an ethnic group of people spread over many countries, mainly in West Africa. Our samples are from Tindangou area, Banfora area (Burkina Faso), Bongor area, Linia area (Chad), Diafarabé area (Mali), Tcheboua area (Cameroon), Banfora area, Diffa area, Zinder area, Ader area and Abalak area (Niger). Using kit Signet Y-SNP Identification Systems and Luminex instrument with LabMAP Luminex Technology we detected particular Y chromosome's SNPs. LabMAP Luminex Technology is universal array platform, which as a probe using fluorescent polystyrene microspheres. We have observed 12 different haplogroups. Haplogroup E, which is typical African haplogroups, is determined with derivated allele in polymorfism M96. We have detected haplogroup E in maximum of 89,3% in the Fulani's subpopulations. In 7,8% we have detected haplogroup R, which is characteristic of populations in the Euroasia. Gene pool of Fulani's population is influenced with a...
|
|
Analysis of the number of persons killed by fires since 2001 in the South Bohemian region and draft measures for the number reduction
ŽIDKOVÁ, Jana
The Bachelor?s Thesis themed ?Analysis of the number of persons killed by fires since 2001 in the South Bohemian region and draft measures for the number reduction? is an analysis of persons killed by fires from 2001 to 2010 in the South Bohemian region according to criteria defined in advance. Among these criteria belongs a comparison of persons killed by fires according to age, buildings, regions of South Bohemia territory depending on months in the year, fire occurrence cause and comparison to other regions of the Czech Republic. First part of the Bachelor?s Thesis is focused on actual number of persons killed by fires in the Czech Republic. It further deals with basics of fires, defines danger in the place of fire and classifies toxic substances occurring as combustion products. Results of the analysis of fires during which there were killed persons in the South Bohemian region according to statistical data are presented in the second part of the thesis. Based on an identification of risk areas there has been developed a system of measures, which should lead to reduction of the number of death by fire. Comparison of the data from 2001 to 2010 brings us to the conclusion that despite the decreasing number of fires the number of killed persons increases and in terms of the death in fires households are the most risky area.
|
|
Extrémní šířkové přírůsty smrku na gradientu nadmořské výšky na Šumavě a jejím podhůří
ČEJKOVÁ, Alžběta
Extreme radial growth reactions were analyzed over a 79-year period (1922-2000) to compare response of Norway spruce (Picea abies [L.] Karst.) along an altitudinal gradient (376-1221 m a.s.l.) in the Šumava Mountains, the Czech Republic. The comparison of the pointer years showed a specific pattern for altitudinal zones. Negative pointer years were usually induced by summer drought at low elevations and by wet-cold summer at high altitudinal zone. These two main limiting factors were probably combined at the middle altitudinal zone. Detailed understanding of the extreme tree ring pattern along the altitudinal and geographical scale may be used as one of the additional indicators of dendrochronological dating and provenance identification of spruce sample among altitudinal zones in the Šumava Mountains.
|
|
Provenance and content characteristics of the Library collection in castle Biskupice
Bendová, Lenka ; Mašek, Petr (advisor) ; Buřilová, Marcela (referee)
(anglicky) This bachelor's thesis processes the library collection in Castle Biskupice. The aim of this thesis consists in the provenance and content characteristics of the library collection in Castle Biskupice. The introduction describes the present situation of the library. The second chapter deals with the library's history. The third chapter outlines the gradual creation of the library and the history of the families that had built it. In the Chapter ˝Provenance characteristics of the collection, an identification of books owners from the family lines of Schaffgotsch, Pálffy, Thurn-Taxis, de Linge and their genealogical relations is provided. Furthermore, this chapter characterizes the collections of particular family members. Partially, it also extends to the collection content characteristics that are provided in general in the follow-up chapter. It divides the collection into particular genres and describes interesting books and authors whose works are to be found in the castle library. The conclusion contains the thesis summary, achieved results and outcomes.
|
|
Determination of clay pigment provenance rased on crystal structure characterisation
Švarcová, Silvie ; Bezdička, Petr ; Hradil, David ; Žižak, I.
Application of X-ray diffraction techniques (XRD) in material analysis of artworks is beneficial in several aspects. Primarily it provides indisputable identification of crystal components found in colour layers, further it makes quantification of identified phases possible and last but not least it can also bring insight in material crystal structure. The crystal structure of materials is often affected by conditions which have been formed under, e.g. geological genesis, manufacturing procedure or secondary changes for example resulted from corrosive action of environment. The knowledge of the relation between the crystal structure of the material and its formation can be useful not only for art historic evaluation of the artwork but also for the artwork restoration and/or conservation. Within the example of clay mineral kaolinite, we show that its crystal order evaluation (based on XRD) can serve well to find its natural origin. We found kaolinite, the main component of kaolin (white earth), in a preparation layer of a Gothic wall painting in St. Maria-Magdalena Church in Bor near Karlovy Vary, where important kaolin deposits are situated. Comparing eight reference kaolins, we demonstrated that these can be differentiated just according to the kaolinite crystallinity (crystal structure ordering). Within this study we compared laboratory powder X-ray micro-diffraction (micro-XRD) with synchrotron radiation X-ray diffraction (SR-XRD). We found that both techniques led to the same results.
|
| |
|
Organic compounds in PM1 aerosol in Kladno-Svermov in winter 2017
Křůmal, Kamil ; Mikuška, Pavel ; Večeřa, Zbyněk
The work deals with the measurement of organic compounds and organic markers used for the identification of main aerosol sources in PM1 aerosol in a small town (Kladno-Švermov) near Prague. Monosaccharide anhydrides and resin acids (emissions from biomass combustion) were found especially. Polycyclic aromatic hydrocarbons (emissions from traffic and incomplete combustion), hopanes (traffic, coal combustion), steranes (traffic), saccharides and alkanes were other groups of analysed organic compounds.
|
|
Sorption Ceramic Membranes with a Functionalized Surface Layer
Zub, Yu.L. ; Tomina, V.V. ; Melnyk, I.V. ; Stolyarchuk, N.V. ; Nazarchuk, H.I. ; Sliesarenko, V.V. ; Sliesarenko, V.M. ; Topka, Pavel ; Šolcová, Olga
In the current work, we have considered and compared the deposition on the surface of ceramic membrane of the polysiloxane layers with different functional groups, such as ≡Si(CH2)3SH, ≡Si(CH2)3NH2, and ≡Si(CH2)2P(O)(OH)2, and ≡Si(CH2)3NHC(S)NHC2H5. We have also reported the fabrication of ceramic membranes with bifunctional polysiloxane surface layers, containing two types of complexing groups of different nature, or (fluoro)alkyl groups along with complexing groups. Tetraethoxysilane and bis(triethoxy)silane structuring agents were used in the study. The identification of the deposited layers on the membrane surface was carried out by IR spectroscopy. It was shown by SEM, that the functionalized layers themselves are usually formed by 50-70 nm nanoparticles and their porous structure depends on several fabrication factors.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22014090110370 - PDF
|
|
Characterization of organic compounds in PM1 aerosol in Kladno in winter 2016
Křůmal, Kamil ; Mikuška, Pavel ; Večeřa, Zbyněk
In this work the organic compounds and organic markers used for the identification of main aerosol sources were measured in PM1 aerosol in a small town (Kladno-Švermov) near Prague. Monosaccharide anhydrides and resin acids (emissions from biomass combustion) were found especially. Polycyclic aromatic hydrocarbons (emissions from traffic and incomplete combustion), hopanes (traffic, coal combustion), steranes (traffic), saccharides and alkanes were other groups of analysed organic compounds.
|
guest ::
