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Mesenchymal stem cells and the possibility of their transdifferentiation into insulin producing cells.
Dostálová, Veronika ; Holáň, Vladimír (advisor) ; Čečrdlová, Eva (referee)
Mesenchymal stem cells (MSCs) have been demonstrated in almost all tissues of the body. Their main source is bone marrow and adipose tissue. These cells are multipotent, e.g. they are capable of differentiating into a variety of cell types. They are able to migrate into damaged tissues. Their other relevant property is a specific suppression of imunity. In the body they serve as precursors for specialized cell types and they also participate in formation of specific tissue microenvironment. Their properties represent a great potential in a wide range of clinical therapies. Besides other possible applications they could be used in the therapy of diabetes mellitus type one. During this disease insulin producing -cells are destroyed. MSCs have been used in experimental in vitro and in vivo studies to differentiate into insulin producing cells. However these cells are not able to produce sufficient amounts of insulin to exclude the supportive administration of exogenous insulin. Therefore there is a need for further research in this field of possible therapy.
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Orphan Nuclear Receptor TLX (NR2E1) in Regulation of Cell Reproduction and Differentiation
Raška, Otakar ; Kostrouch, Zdeněk (advisor) ; Otová, Berta (referee) ; Nedvídková, Jara (referee)
Nuclear receptors constitute a large family of transcription factors that are powerful regulators of animal tissue metabolism, homeostasis, tissue maintenance and development. They are particularly attractive for their ability to respond to the binding of hormones, metabolites, xenobiotics and artificially prepared molecules and transmit the interaction with these small lipophylic molecules to specific regulatory potential. In search for nuclear receptors that are likely to be critical for neural tissues in invertebrates and conserved during the evolution of animals, we have identified a close homologue of vertebrate TLX in a planarian Schmidtea mediterranea. Planaria represent very promising biological model systems for studies on tissue maintenance and regeneration. Planaria are able to resorb their tissues and use them as sources of energy during fasting and they re-build their bodies from neoblasts when food is plentiful. Our search in Schmidtea mediterranea's publicly accessible genome sequencing data indicated that planarian genome contains at least one gene with a high degree of similarity to vertebrate TLX. We cloned full length CDS (coding DNA sequence of cDNA) and characterized the gene functionally. This showed that the planarian and vertebrate NR2E1 are highly similar...
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Alternative mathematical notation and its applications in calculus
Marian, Jakub ; Pick, Luboš (advisor) ; Zahradník, Miloš (referee)
We explore the possibility of formalizing classical notions in calculus without using the notion of variable. We provide a new mathematical 'language' capable of performing all classical computations (namely computing limits, finite differences, one-dimensional derivatives, and indefinite and definite integrals) without any need to introduce a variable. Equations written using our notation contain only func- tion symbols (and as such are completely rigorous and don't leave any room for vague interpretations). They also tend to be much shorter and more mathemati- cally transparent than their traditional counterparts (for example, there is no need for introduction of new symbols in integration, and definite integration is formalized in such a way that all rules (including 'substitution' rules) for indefinite integration translate directly to definite integration). We also fully formalize the Landau little-o notation in a way that makes computation of limits using it fully rigorous. 1
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School Achievement Motivation
Smolová, Alžběta ; Pavelková, Isabella (advisor) ; Hříbková, Lenka (referee)
The thesis is focused on the issue of school achievement motivation. It is an empirical work. The theoretical part of thesis introduces the concept of school achievement motivation based on the general concept of achievement motivation (e.g. Atkinson's and Heckhausen's model of achievement motivation), which is introduced at the beginning of the theoretical part. Forming of school achievement needs is emphasized within the concept of school achievement motivation. The last chapter presents the previous research works on achievement needs creating a connection to the empirical part. The purpose of the empirical part of the thesis is to explore achievement needs of pupils of upper primary school and determine whether these needs are differentiated. The results of research indicate that in connection with the development of achievement motivation initially generalized achievement needs differentiate within the school subjects. The need to avoid failure differentiates less in comparison with the need to achieve success. Differences in the achievement needs related to the age, gender, English speaking and English non speaking classes are discussed as well. Keywords: school achievement motivation, differentiation, upper primary school, need to achieve success, need to avoid failure
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Targeted differenciation of limbal and mesenchymal stem cells and their therapeutic application
Kuthanová, Hana ; Holáň, Vladimír (advisor) ; Indrová, Marie (referee)
The research of stem cells slowly transfers from the experimental to the preclinical and clinical level. They are in the centre of interest thanks to their potential to treat many of severe injuries and genetically determined diseases. However, the clinical application of these cells has to be based on a basic research of their characteristics and differential potential. Adult stem cells are in organism in minor populations in unique niches. In comparison with embryonic and induced pluripotent stem cells, the adult stem cells have lower differential potential but they also tend less to making teratomas. The therapeutic use of differential and transdifferential potential of limbal and mesenchymal stem cells is described here in more detail with focus on their use in damaged ocular surface treatment. Limbal stem cells are the only source of stem cells for corneal epithelium regeneration in most organisms. Deficiency of these stem cells leads to severe eye disorders even to blindness. Nowadays, a transplantation of allogeneic limbal stem cells or allogeneic limbus is the only chance for patients with total limbal stem cell deficiency. In clinical trials with patients with particular limbal stem cell deficiency, autologous limbal stem cells were successfully transplanted. Mesenchymal stem cells derived...
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Ethnic differentiation of fertility in Kazakhstan
Abuov, Anas ; Kučera, Tomáš (advisor) ; Burcin, Boris (referee)
Ethnic differentiation of fertility in Kazakhstan Abstract This paper examines ethnic differentials in fertility in Kazakhstan using data from the Statistical Agency of Kazakhstan from 1999 to 2006. The study focuses on four major ethnic groups including Kazakhs, Russians, Ukrainians and Uzbeks which account for 90% of the country's population. The paper lays emphasis on comparison and determination of differences in fertility trends among the chosen ethnic groups. In order to describe the main reasons of differences in fertility trends in greater detail, the author analyzed birth orders in fertility in the groups. At the same time, the paper presents ethnic structure of Kazakhstan's population and within each ethnicity it shows age structure which also determines fertility trends. Keywords: Ethnicity, ethnic groups, fertility, differentiation, Kazakhstan.
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Applied theory of inclusive education in primary schools with focus on students with specific learning disabilities
Kalinová, Tereza ; Stará, Jana (advisor) ; Kargerová, Jana (referee)
The aim of my thesis is to analyze various aspects of the inclusive teaching concept within the first stages of elementary education and how such aspects may be applied. The work is divided into two main parts, theoretical and practical. The theoretical part is further divided into five main chapters. The first chapter covers the topic of how all students can be included in the elementary education system. The second chapter discusses the key components of how the theory can be implemented and successfully applied to individuals in order to help students achieve their educational goals. The third chapter focuses on topics of special needs education, learning and behavioral disorders. The fourth chapter discusses the necessary role parents and guardians should take in this process. A summary of theoretical part is provided in the fifth chapter. The practical part of the thesis is also divided into four chapters, each of which is numbered and connected to the theoretical part. The sixth chapter defines the objectives of the practical part of my thesis, which are based on various research methods including interviews, surveys and questionnaires. The purpose of the research was to collect firsthand accounts, information and data that would help me analyze the concept of inclusive education and assist...
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Origins of vertebrate hematiopoiesis
Svoboda, Ondřej ; Bartůněk, Petr (advisor) ; Divoký, Vladimír (referee) ; Živný, Jan (referee)
(ENGLISH) Hematopoiesis is dependent on the actions of hematopoietic stem cells (HSCs). This process is tightly controlled through a complex array of extrinsic and intrinsic factors. Even though the hematopoiesis seems to be well conserved across the disparate vertebrate animals, erythroid and thrombocytic differentiation have changed during the evolution of mammals. Specifically, adult mammalian red blood cells have the unique feature of being enucleated, and mammalian thrombocytes are not individual cells, but fragments of megakaryocytes, instead. It is likely that these enhancements provided a survival advantage to early mammalian species; however, they also bring up the question of evolutionary origin of these cells that studied using zebrafish (Danio rerio) model. First, it was necessary to generate a toolbox of a recombinant cytokines and optimized culture media that allowed us to manipulate zebrafish hematopoietic cells ex vivo in liquid and clonal cultures. Interestingly, teleost species underwent an extra duplication event during their evolution and as a result, two copies (paralogs) of some of the genes are present in zebrafish. This was also the case for majority of the cytokines from our toolbox and here, we provide functional characterization of these paralogs. Strikingly, our results...
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Differentiation of yeast colonies and development of new approaches to monitor oxygen and nutrient availability
Vopálenská, Irena ; Janderová, Blanka (advisor) ; Demnerová, Kateřina (referee) ; Pichová, Iva (referee)
Yeast Saccharomyces cerevisiae as an unicellular organism is one of the best-studied experimental organisms. It is an important model organism for the study of intracellular processes of eukaryotic cells. Yeasts are also social organisms with cell-to-cell communication able to form organized multicellular structures (colonies and biofilms). Yeast and other microorganisms in nature prefer to form colonies on solid substrates rather than to grow as "planktonic" single cells (Palková, 2004; Wimpenny, 2009). The yeast S. cerevisiae typically forms colonies, biofilms were described only rarely. Yeast colonies exhibit an organized morphological pattern characteristic of each particular yeast strain (Kocková-Kratochvílová, 1982). This work is focusing on morphology and differentiation of the S. cerevisiae colonies of common laboratory strains forming less structured colonies, and strains of the Σ1278b genetic background forming highly structured "fluffy" colonies. It shows that polarized budding pattern and especially cell ability to form aggregates enable development of structured morphology. During development of "fluffy" colonies two differently regulated events of dimorphic switch from yeast form to filamentous growth occur. One of these events is dependent on the surface glycoprotein, Flo11p flocculin. This...
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The Proposal of Company Development
Semančiková, Karolína ; Šiarnik, Pavel (referee) ; Bayerová, Vladimíra (advisor)
The bachelor´s thesis serves as a proposal of a strategy for company´s further development. The company´s main business activity is production of cosmetic products. The starting point for this thesis is analysis of actual situation of the company and its processes. The thesis has three parts while the first one is theoretical and deals with the question of strategy proposal and strategic analysis, in the second part there is an analysis of the current condition of a company and the third part offers a practical suggestion of company´s options and steps it can take in order to achieve further development.
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