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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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Chosen natural resources: focuses of conflicts nad its economic consequences
Sytný, Patrik ; Vošta, Milan (advisor) ; Sankot, Ondřej (referee)
The bachelor´s thesis aims to three chosen natural resources, which are petroleum, natural gas and water, and conflicts based on disputes over them. The aim of the thesis was to valorize nature of the conflicts, define their main focus and draw prospective economic consequences. Professional literature that deals with this kind of issue, was used in order to find out the true nature of the conflicts. The result is an evaluation, which has proven, that the most influental conflicts are rising over disputes over petroleum. Their economic and political impacts are very perceptible and economically measurable in the way of GDP, the total product and other economic indicators. In the thesis has been also proven that the conflicts on the basis of natural gas have similiar nature and extent as it is in the case of petroleum, even thought in a smaller amount. The least important conflicts in a meaning of economic impacts are those over water, by those has been proven, that they do not poses such an impact on economic and political situation as petroleum or natural gas nowdays.
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The nutritional importance of polyphenols in berries
MARTANOVÁ, Anna
People's nutrition is inseparable part of our lives. We could include substances, that we know are healtful, to our food. If we don't have enough of the substances, we can see negative effects on human organism. The substances are named micronutrients and vitamins. We could enrich a diet with substances that we think are healthy, but their deficit doesn't case pathological symptoms. Food which we put to this category is possible to name as function food. Function food contains substances which have positive effect on our health. One of the substances are polyphenols. This issue is very interesting for me, because polyphenols are compounds that are among others particulary found in berries, especially in blueberries. Blueberries are berries that are widespread throughout the world. One of the best sources of blueberries is also Sumava, where I come from and where blueberries are well connected. The first part of my thesis is about the functional foods and ingredients that make these foods Functional foods. Ordering: probiotics, prebiotics, synobiotiks, micronutrients, fytochemiks and polyphenols. Farther the first part deals with berries, specializing in blueberry and its properties and composition, which can have a positive impact on human health.In the second part there were two objectives of the work. The first aim of my study was to determine the diet, which includes foods that contain the optimum intake of polyphenols, equivalent to 120 ml of fetal native blueberries. Polyphenols are that part of blueberries, which make them so special. In the diet were preferentially embedded short dishes that have undergone heat treatment. Analyses of polyphenols in foods and beverages I got with the program GEOMED hospital Ceske Budejovice, Inc., Department of Clinical Pharmacology. Results polyphenol content were performed by HPLC. The content of polyphenols in native blueberries was laid as equal to 1 and the contents examined further product was then expressed as a multiple or ratio of the native blueberries. I take into account all these factors when drawing up the model menu. Another aim I have set is to map products in which the content of polyphenol is beneficial. There is a high number of products with blueberry flavour on the market. The content of blueberries, however, is often negligible. The surprising result was the polyphenol content in lemonade Toma, whose relative value ug / ml is 2.59 when compared with the native blueberry, which is placed as equal to 1. After the development work, in which I obtained information from the literature search, the sales network and the Department of Clinical Pharmacology, Hospital Ceske Budejovice, I would like to say that blueberries are an important source of polyphenols and that a diet which will include daily equivalent of receiving polyphenols, may not in any way differ from a regular rational diet. Not even cooking the dish precludes putting together this diet because blueberries are relatively stable against temperature changes. Inclusion of such a diet in the catering equipment could bring a positive impact in the future, especially for older people by improving memory function and balance.
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Analysis of the Motivation and Satisfaction of Employees in a firm
Gonta, Natalya ; Dvořáková, Zuzana (advisor) ; Odvárková, Markéta (referee)
The aim of this study is to analyze the motivation and satisfaction of employees at British American Tobacco Ltd. The bachelor thesis consists of two parts - theoretical and practical. The first part describes the concepts and sources of motivation, which affect the performance of employees in the company. The second part describes the aim of the research, its progress and evaluation results in respect of which was carried out to analyze the way of motivation and satisfaction of employees in the company. For the following analysis methods were used questionnaire and a personal unstructured interview. The result of the thesis is mentioned proposal for improving the motivation of employees, which has potential and could be implemented in the company.
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The Issue of Nursing Care in Old People's Homes
MARKOVÁ, Štěpánka
The thesis deals with the issue of nursing care in old people's homes. Given the improving medical and nursing care and also the prevention of diseases, the human life extends. This leads to the aging of population. Therefore, it is essential to understand this issue, its interconnection with nursing care, but also to find the right approach to the elderly in residential facilities providing social services. The theoretical part of the thesis deals with old age and aging, behavioural changes in old age, specifics of communication with the elderly, long-term care for the elderly, care for a man in nursing homes. It deals with the work of nurses in old people's homes and with their education. Five objectives were set within the framework of the research. The first objective was to determine areas of nursing care, in which the nurses working in old people's homes have most problems. The second objective was to find out how the nurses manage physical strain. The third objective was to determine how the nurses manage psychical strain. The fourth objective was to find out what theoretical knowledge and practical skills the nurse must have. The fifth objective was to identify possibilities of further training for nurses working in old people's homes. Qualitative research was applied in the empirical part of the thesis. The method of non - standardized interview was used. The research group consisted of 10 nurses who work in residential facilities for the elderly in old people's homes. To process the interviews, the open coding technique was applied - a paper-and-pencil method. To complement psychical resilience of nurses, S.O.C questionnaire by A. Antonovsky was used. There were 42 questionnaires used in the research. The interviews with the respondents showed that the work of a nurse in homes for the elderly is physically and mentally demanding. And it is obvious that nurses feel the mental strain as more damaging. The physical demands are connected with the state of the client and his diseases but also with the space limitations of homes and night shifts. The mental burden is caused by similar factors and also by the family of the client, specifics of the communication with the elderly, cooperation with the hospital, working atmosphere, responsibility and keeping records. Next important factor that negatively influences mental state of the nurses is a lack of prestige and recognition of their work. Even though the nurses feel both types of stress, most of them cope with it without problems and they try to restore their balance in their leisure time. The conclusions of the thesis also point to the high demands and wide range of expertise and skills required from the nurses when providing care. Even specialized procedures are carried out in homes. Nurses must know how to communicate, handle conflict situations, co-operate with colleagues from different fields. Great emphasis is placed on independence in evaluating the health state of the client while ensuring the nursing care. The nurse must have personal qualities for work with the elderly. Another important component of their work is their knowledge and practical skills in the field of social work theoretically backed by the Act 108/2006 Coll.
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The Conclusion of a Contract under the United Nations Convention on Contracts for the International Sale of Goods in Comparison with Czech National Legislation
Krčálová, Kristýna ; Boháček, Martin (advisor) ; Levý, Jan (referee)
This Master's thesis focuses on the United Nations Convention on Contracts for the International Sale of Goods (CISG), better known as the Vienna Convention. The main purpose of this thesis is to evaluate conditions for application of this international treaty and to assess the contracting process under the CISG including the similarities and differences with the Czech national legislation. Finally, it seeks an answer to the question, which of these rules (international or national) are more advantageous for a Czech contractual party with respect to concluding a contract. The thesis is divided into five parts. The first one represents an introduction to the CISG, its origin and the Czech Republic's attitude to it. The second part identifies the conditions for application of the CISG and therefore defines a subject and an object of an international contract of sale under the CISG. Both third and fourth parts gradually introduce two phases of the contracting process (offer and acceptance) including the relevant case-law. The last chapter strives to complete the contracting process with an analysis of the exact moment, when a contract is concluded, as well as with an analysis of a potential battle of the forms and its impact on a contract conclusion.
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The current level of compliance with the concept of prenatal care from the perspective of pregnant women
URBÁNKOVÁ, Martina
This bachelor thesis deals with a current level of abidance of the prenatal care conception from the point of view of pregnant women. Theoretical part is divided into four main chapters. The first chapter is focused on the history of midwifery and prenatal care. The second one describes prenatal care in general, organisations, goals, antenatal preparation by the midwife, pregnancy card and a role of the midwife in the prenatal consulting. The third chapter deals with pregnancy diagnosis and the last one concerns the examination of pregnant women in each trimester. Practical part of the thesis points out a comparison of a current level of abiding the prenatal care conception in České Budějovice and Benešov region.Three goals were set prior to writting this thesis. The first goal was to find out the abidance of the prenatal care conception from the point of view of pregnant women. The second goal was to find out, whether pregnant women understand the importance of all the examinations carried out during prenatal consultings. The third goal was to find our and compare the level of abiding the prenatal care conception in České Budějovice and Benešov region. Three questions arose from these three goals: What is the level of abiding the prenatal care conception? What examinations are being carried out during prenatal consultings? What is the difference between abiding the prenatal care conception in České Budějovice and Benešov region?The qualitative method had been used to conduct the survey.Answers to the questions from the survey were searched during the acquisition and processing of the semi-structured interviews. Conclusion of the survey is, that all the pregnant women are satisfied with the current conception of the prenatal care. This way of timing of visit intervals in prenatal consultings seems right to them, important and necessary, not only for a control over themselves, but also for a control over the fetus. The level of the conception of prenatal care is good, satisfactory for all the mothers. Surprising result was, that half of the mothers is unable to describe and elaborate what examinations they went through in prenatal consulting, when it was and what was its purpose. Especially when only two respondents complained about insufficient awareness from the physician about performed examinations. As the most common examinations were stated: blood pressure, urine, weight, CTG scan and vaginal examinations. Based on the conducted survey I have managed to find out that the frequency of regular visits in prenatal consulting, according to CGOS recommendations in České Budějovice was abode only in two cases. This fact was not confirmed by any respondent from the Benešov region. Schedule of the regular visits depends on physician´s consideration. Another difference was not discovered. Current level of abidance of the prenatal care conception in Benešov region is similar to České Budějovice.The thesis may serve as an informational material in prenatal consultings in the future, especially for pregnant women, who want to know the progress of prenatal care.
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Sorption and Stabilization of Metals/Mettalloids by Innovative Synthesized Sorbent Amochar.
Ouředníček, P. ; Trakal, L. ; Komárek, M. ; Pohořelý, Michael
Remediation of contaminated soil which is based on stabilization and immobilization of potential\nhazardous substance by sorption materials has been studied intensively nowadays. Biochar – activated organic carbon belongs to this group of stabilizing agents which can adsorb wide range of contaminants, including metals/metalloids. Surface area of the biochars is quite large in general and functional groups (e.g. COO–) can form chelates or alkaline elements on the surface, which is represented by cation exchange capacity. Altogether with the high pH values (7.00 – 10.00), biochars are quite effective sorbents and can adsorb metals/metalloids from the solution (ground water), especially in acidic soils (in the environments affected by intensive mining activities). Sorption\neffectiveness can be increased (especially for As (V) or Cr (VI) sorption) by modification of biochar by various types of secondary oxides. Innovative sorbent AMOchar (AMO + biochar) has been synthesized currently. The product was prepared by adding of biochar to the reaction solution during amorphous manganese oxide (AMO) synthesis. The AMOchar was formed mainly by Mn-oxalates which had coated surface of the pristine biochar. AMOchar composite was able to remove significantly higher amounts of various metal(loid)s from the solution despite the rather high pH of the material. Sorption effectiveness was high not only in case of Pb (II) sorption (almost 99%), and Cd(II) (51.2%), but also a very high amount of As(V), 91.4%. Additionally, both AMOchar composite was able to reduce Mn leaching. This can avoid potential post-contamination caused by the dissolution of less stable Mn-oxalates as observed in the pure AMO.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_22316111113040 - PDF
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Analysis breeding of milk cattle on the chosen biofarme
Plášková, Pavlína ; Toušová, Renata (advisor) ; Ducháček, Jaromír (referee)
The aim of this thesis is to evaluace the conditions of dairy cattle breeding in ecological agriculture and to compare them with concrete values taken from the chosen ecological farm Bílčice, which has been part of ecological agriculture since 2011 and which is focused on czech fleckvieh breeding and bio milk production. The first part deals with dairy cattle breed characteristic, main objectives and ecological agriculture principals, welfare problems, breed cattle and utility control, housing technology, nutrition and feeding, mechanical milking technology, milk production, reproduction, health condition and breeding economy. The practical part contains the rating of milk yield index (milk production, % proteins, % fat) and reproduction index ( meantime, perioda service, insemination index and after first parturition percentage). 650 pieces of cos were stabled at farm, from that 200 pices of dairy cos were mostly czech fleckvieh. Monitoring was made in 2013. Dayily milk production was around 2 750 kg. The milk utility for lactacion was in 2013 6 100 kg of milk, 3,89 % fat and 3,34 % proteins. The dairy cos were free stabled with high bedding, They were mechanically milked twice a day in herringbone parlours. Milk is being delivered once in twodays to Olma dairy. Cows are regularly driven out to pasture in summer feeding period. They are extra fed with 10 kg of clover silage and 6 kg of scarp (barely, wheat, triticale). Cows stays in stables for winter time. The ration consists of 45 kg of clover silage, 6 kg of scarp, 2 kg of lupine and 1 kg of corn in first time of lactacion. The cow utility in transitional form of economy at farm Bilcice was 7 477 kg of milk in 2010, a value in the Czech Republic was 7 726 kg of milk. After entering the ecological agriculture in 2013 milk yield at the farm decreased on 6 100 kg of milk and the average value in the Czech Republic increased to 8 370 kg of milk. The cow utility in transitional form was 4,03 % fat in 2010 and 3,89 % in 2013 (ecological agriculture). A diference between 2010 and 2013 is 0,14 %. Proteins were 3,39 % in 2010 and 3,34 % in 2013. There was average value for every reproduction index. The service period was 96 days and the meantime was 400 days, the insemination index was 1,8 and the after first parturition percentage was 54,2 %.
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Lifelong Education as a Part of the Social System
Neckařová, Jana ; Šrédl, Karel (advisor) ; Hana, Hana (referee)
The diploma thesis discusses about education of adult population, while pointing out necessity of education in any age. This thesis explain importance of education of the adult population, mention forms and possible methods which are used in this type of education the most. Besides the theoretical outcome there is practical aspect included as well in form of questionnaires. Investigation in form of research was focused directly on employees, who as well as on employers whose employees attended the program. In summary both parties consider adulthood gained knowledge as a great benefit to employee as well as employer. In any case, people with higher level of education have better chances of getting a possible job than those with no or low level of education.
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