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Lifestyle of patients with peripheral arterial disease before and after surgery
REZKOVÁ, Helena
Theoretical background: Peripheral arterial disease is a serious disease of Loir limb arteries which originates from atherosclerosis. The theoretical part deals with the lifestyle. There are mentioned basic risk factors of chronic forms. Further described are the pathophysiology changes generated on the basis of atherosclerosis and related causes for the origin of the disease. Subsequently there are explained methods of classification, developed clinical symptoms, diagnosis and therapy. This section also looks at the role of a nurse in the care of patients with peripheral arterial disease. Objectives: The aim of this work was to evaluate how patients with peripheral arterial disease changed their lifestyle after invasive vascular surgery. There were provided the following research questions: How do patients with peripheral arterial disease approach to the prevention of risk factors? What is in patients with peripheral arterial disease the main impuls for the possible change of their lifestyle? What influences surgery patient's attitude to risk factors for peripheral arterial disease? How crucial is a lifestyle change in patients after surgery for peripheral arterial disease? Methods: The theoretical part is processed using expert sources. The empirical part of the theses was processed using qualitative research. The chosen technique for data collection was semi-structured interviews with patients, with prepared open questions. The research was done in several phases - the first interview was conducted at the patient admission department (before surgery), followed by the next phase after three months, and 6 months after surgery. The interviews were recorded on a prepared form, then transcribed into electronic form and analyzed by open coding method. Results: The research showed that surgery can sometimes be triggered by changes in the patient's lifestyle. The first interviews in the preoperative period focused on how respondents felt, what was their lifestyle before surgery for atherosclerotic changes of the lower extremities. One of the questions was focused on patient knowledge about risk factors for the development of PAD and possible prevention. The second phase of interviews took place 3 months after the surgery. It dealt with the question about the respndents´ lifestyle, the respondents in the postoperative period, whether there were any changes. Some positive changes occurred in eating and there were some changes in doing physical activity. The third stage was carried out six months after surgery, again in order to detect changes in lifestyle. Minor changes came in smoking habits, the issue of diet and physical aktivity. Questions were also focused on the psychological level. We found out who helped patients and what was most motivating in implementing changes. In the conclusion/summing up question the patients marked themselves ow they managed to make a change. Conclusion: The number of patients with peripheral arterial disease and other cardiovascular diseases is increasing. The basis is the start to modify risk factors, namely tobacco use,dietary habits, physical activity. The medical staff members participate on changes in patients´ lifestyle by appropriate education. The problem occurs when the patient despite the support of close people can not cope to carry a twist in their life. There should a medical staff member offer a professional help to deal with this important step. Practical use: The aim is to draw attention to the expansion of education about lifestyle changes for patients diagnosed with PAD also in the postoperative period. Patients who are released to home care should receive enough information that would convince them to change their attitude towards their health, and that surgery is only one of the ways to prevent further attacks and complications. Results can improve communication with patients and focus on increasing their awareness and possibly offer assistance.
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EF-Tu Protein Domains:Functions and Thermostability
Šanderová, Hana
Charles University in Prague Faculty of Science Department of Genetics and Microbiology EF-Tu PROTEIN DOMAINS Functions and Thermostability PhD thesis Hana Šanderová Specialization: Molecular and Cellular Biology, Genetics and Virology Supervisor: Prof. MUDr. Jiří Jonák, DrSc. Department of Gene Expression, Institute of Molecular Genetics AS CR Prague 2008 Summary of PhD Thesis The bacterial elongation factor Tu (EF-Tu) has been extensively studied for decades as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in structure and function makes EF-Tu proteins suitable for the studies of evolutionary relationships between organisms and for elucidation of the structural features of adaptation to various living conditions. Furthermore, since all known EF-Tu proteins are composed of three distinct domains, they can also serve as suitable models for the understanding of domain organization in proteins. One of the main research projects of the Department of Gene Expression at the Institute of Molecular Genetics AS CR, where I did my PhD studies, was the study of the primary structure, transcription regulation and functions of bacterial elongation factors Tu...
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The study of the role of biotransformational enzymes in chemical carciogenesis
Kondrová, Eliška ; Souček, Pavel (advisor) ; Skálová, Lenka (referee) ; Vobořilová, Jana (referee) ; Bořek Dohalská, Lucie (referee)
In vitro biotransformation studies are an integral part of both toxicological research and drug development. They allow for a significant reduction of tests on human volunteers and provide detailed information about the metabolism of a given compound. Due to limited availability of human liver tissue, it is necessary to make use of alternative model systems and model animal species in the study of the interactions between biotransformation enzymes and xenobiotics. Since the activities of the most important human biotransformation enzymes are absent in the most commonly used laboratory species, rat, information gained in experiments with minipigs is very valuable. Recombinant human biotransformation enzymes expressed in bacteria can be used in biotransformation studies in the form of isolated bacterial membranes. These model systems provide information about the metabolism of a given xenobiotic by a defined enzyme and thein advantages compared to purified enzymes include low cost and quicker and easier preparation with lower loss of enzymatic activity during isolation. The aims of this study were: 1) To prepare a model system with recombinant human biotransformation enzymes expressed in bacterial membranes and to compare the properties of this system with minipig liver microsomes. To assess the usefulness of...
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The thoracic and lumbar spine injury - role of imaging modalities in diagnostic and therapy
DOLEŽALOVÁ, Martina
This article provides information about thoracic and lumbar spine injury with reflection to the role of radiologic assistants. Spine injury belongs to the most serious injuries and its incidence is rising. These injuries are diagnosed using imaging methods like plain X-ray, computed tomography and magnetic resonance imaging. Radiologic assistant himself is the person, who is responsible for the correct examination. If these injuries are treated surgically, radiologic assistant meets them at operating theatres too. Here the assistant manipulates with fluoroscopy machines and helps the operator with procedure navigation. Important is that the radiologic assistant should know all the steps of each procedure and should take care of radiation protection. If the procedure is done inadequately, the patient can be misdiagnosed and the radiation dose for him and for the staff may be increased. Since 1st January 2006 to 31st December 2006, 84 patients (48 women, 36 men) were hospitalised at the Neurosurgery departement of University Hospital Hradec Králové under diagnosis of thoracic or lumbar spine fracture. The age, frequency of vertebral fractures in thoracic and lumbar region and percentual use of various therapeutic methods are noted. But the most important aim of the study are imaging methods used in these patients and mistakes done during these examinations.
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New biomarkers in diagnostics and monitoring of immunopathological inflammation in patients with psoriasis.
Volencová, Soňa ; Jílek, Petr (advisor) ; Drahošová, Marcela (referee)
Psoriasis is one of the most frequent cutaneous diseases, the prevalence of psoriasis in population is around 2-3%. Diagnosis is based on typical manifestation, anamnesis and histopathological examination. Effectality is assessed by localization, course and measure of the disease. Plaque psoriasis appears as raised areas covered with silvery white scaly skin. T-lymphocytes play the most important role in immunopathogenesis of psoriasis. There is a prevalence of Th1 lymphocytes. Genetics factors have a role in predisposition to disease. Psoriasis is associated with the alleles from the MHC. In the treatment of psoriasis is usually used topical and systemic therapy and phototherapy. Biologic treatment of psoriasis is now possible due to the method of molecular genetic. We determine concetrations of sCD28, sCD30, endoglin, MCP-1, Apo-1/fas and Fas ligand by ELISA. Follow-up file was made by 38 patients. We investigated importance of these markers for the diagnosis and monitoring of psoriasis. We detected that Apo-1/fas and sCD28 are suitable markers for assignation of diagnosis, while Fas ligand, sCD30 and endoglin may be used for monitoring of disease and evaluation the effectivity of therapy.
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Radiodignostics of breast cancer
Bahenská, Kristýna ; Lázníček, Milan (advisor) ; Melicharová, Ludmila (referee)
Kristýna Bahenská Radiodiagnostics of breast cancer Vedoucí diplomové práce: Prof. PharmDr. Ing. Milan Lázniček, CSc. Mamodiagnostics is today the only way of oncoprotection dealing with breast cancer . It is also the single tool applicable to decrease mortality of this disease. For further development of breast cancer radiodiagnostics very important role have nuclear medicine and also improvement of imaging methods. These are the contemporary ways helping in more precise diagnostics of breast cancer disease.
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Deep vein thrombosis and role of selected mutations for the coagulation factors genes
Čtveráčková, Zuzana ; Šimůnek, Tomáš (advisor) ; Dršata, Jaroslav (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Zuzana Čtveráčková Supervisor: Doc. PharmDr. Tomáš Šimůnek, Ph.D. Title of diploma thesis: Deep vein thrombosis and role of selected mutations for the coagulation factors genes. This thesis is focused on mutations for the coagulation factors genes that can lead to deep vein thrombosis. In the first part, we describe the role of hereditary thrombophilia in the development of disease. Following is the description of each thrombophilic mutations and their meaning which is not always clear. The following mutations were detected: Factor V Leiden (G1691A), factor V R2 (H1299R), factor II prothrombin (G20210A), MTHFR (C677T, A1298C), factor XIII (V34L), PAI-1 (4G/5G) a EPCR (A4600G - haplotype A3, G4678C - haplotype A1). Most of them have been previously identified as risk factors for deep vein thrombosis, but specifically the factor XIII V34L polymorphism is considered to a protective factor. Other mutations with protective effect could be one of the genotypes of the PAI-1 and EPCR A1 haplotype. In the practical part, the samples and the results of 783 subjects were used. First part of them which was diagnosed by reverse hybridization commercial detection kit CVD Stripassay T (ViennaLab)...
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Family and School Socialization of Children with ADHD
Bílková, Zuzana ; Pavelková, Isabella (advisor) ; Vágnerová, Marie (referee) ; Miňhová, Jana (referee)
TITLE:Family and School Socialization of Children with ADHD AUTHOR: Zuzana Bílková DEPARTMENT: Department of Psychology SUPERVISOR: Doc. PhDr. Isabella Pavelková, CSc. ABSTRACT: The thesis is focused on the process of socialization of children with ADHD in family and school setting. We present the overview of current research about etiologies, symptoms and therapy of the syndrome. The socialization is defined as the process of interactions between individual and his social and cultural background. ADHD symptoms are considered to be an important factor in socialization process, particularly as the factor affecting the relationships with parents and teachers. The research is focused on children of preschool and school age. We pose research questions concerning the influence of setting the diagnosis, the influence of ADHD on child's relationships with educators and the influence of the diagnosis on the relationship between family and school. Data were collected during interviews with parents and teachers of children with ADHD and during observation of children in their home or school. Data were analysed by qualitative analytic methods. Research outcomes point out the importance of educators' attitudes towards the problem, the importance of early setting a diagnosis and important role of motivational...
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The opinions of pregnant women of current possibilities of prenatal diagnosis.
DYKOVÁ, Iva
This bachelor thesis deals with the view of pregnant women on prenatal diagnosis. Prenatal screening includes many tests, where the aim is the versatile safety of pregnant women.The theoretical part of the bachelor thesis focuses on the pregnancy period which for most women is the most beautiful period during which the fetus develops in her organism.The purpose of prenatal care is primarily the consistent prevention of any possible pathological situations and timely capture of deviations from the normal course of gestation, with ensuring all available diagnostic and therapeutic measures. The goal of the prenatal diagnosis of congenital developmental defects is to detect congenital developmental defects as soon as possible and to help further approach strategy to pregnant women.Prenatal diagnosis is nowadays an integral part of prenatal care. It is subject to great demands in terms of safety and accuracy.The ethical viewpoint of prenatal diagnosis is just as important as technology by which testing and invasive performance tests are carried out. Prenatal genetic diagnosis has several important ethical issues. An important ethical procedure is proper information and consent of the pregnant woman to the testing, the interpretation of results, maintenance of privacy, medical confidentiality, the impossibility of abusing identified results, availability of further examinations and follow-up treatment at the highest level. The termination of gravidity in the event of statuses incompatible with life, or with permanent damage of the fetus, may be carried out solely at the request of the pregnant woman. The fundamental ethical issue is whether the fetus is regarded as an individual human being. Another dilemma resulting from this is whether a woman can take a decision for herself and the fetus, or whether the fetus is already a human being with its own rights.Furthermore, the theoretical work is devoted to the role of midwives in prenatal care. When providing care for pregnant women in the Czech Republic, the most commonly applied system is the close cooperation of the midwife with the physician. An integral part of the work of a midwife is the identification of anamnestic data by the nursing process method relating to pregnant women in the somatic, psychological, social, and also in the spiritual area. The objective of the bachelor thesis was to find out how pregnant women perceive testing carried out during pregnancy and how they experience situations where the invasive testing of congenital developmental defects of the fetus was recommended to them. The qualitative investigation using the interview method was used for the research investigation. An in-depth structured interview was selected for the data collection technique. The research group was composed of five pregnant women who had a positive screening to congenital developmental defects in I. or II. trimester and had to decide whether to undergo or not invasive testing to detect congenital developmental defects of the fetus. The research results have shown that women consider pregnancy screening tests as a compulsory part of the care for pregnant women. Women perceive non-invasive testing as safe and beneficial for both the fetus and the woman herself. Invasive testing is perceived by most of the women as beneficial despite all risks. During interviews we also met a woman who wouldn't accept any risks and therefore refused invasive testing. Despite the fact that every woman has the right to refuse such testing, this respondent noted how inappropriately most of the doctors and midwives were behaving towards her afterwards. The results also showed that women to whom invasive testing had been recommended experienced fear for the child and future pregnancy. According to the respondents, information in prenatal counselling proved to be clearly insufficient.
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The introduction of diagnosis of new bacterial pathogens Inquilinus and Pandoraea isolated from patients with cystic fibrosis and determining their sensitivity to antibiotics
Šulcová, Romana ; Melter, Oto (advisor) ; Nyč, Otakar (referee)
Cystic fibrosis is one of the most common autosomal recessive hereditary disease. Nowadays, people, with this disease, because early diagnosis and treatment of other associated symptoms a better prognosis than a few years ago. CF is a disease that is subject to mutation of the CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) on the seventh chromosome. Defect in the CFTR protein leads to failure of resorption and secretion of electrolytes chloride channel and thereby creating viscous mucus in the lungs, which is the main cause of chronic bacterial infections of the lower airways. Among the most common pathogens causing severe respiratory infections in CF patients include: Staphylococcus aureus, Pseudomonas aeruginosa, Burkholderia cepacia complex. In patients with CF is now showing in the airways of new bacterial species whose biological properties are not yet known and their role in the etiopathogenesis of the disease and epidemiology are not exactly known. In these days their detection is used by specific amplification methods, or sequencing of the 16S rRNA gene. Emerging pathogens such as the genus Pandoraea and Inguilinus that belong non-fermenting Gram-negative groups in the rods, and therefore are often exchange for other bacterial species that are phenotypically similar to them. In the...
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