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Changes to the contents of proline and relative electrolyte leakage of the selected genotypes of poppies depending on the influence by low temperature
Kudrna, Jiří ; Hnilička, František (advisor) ; Orsák, Matyáš (referee)
The object of this thesis is to study the effect of low temperatures, proline content and relative elektrolyte leakage in a selected range of varieties of opium poppy.
Experiment included 18 poppy varieties with different contents of morphine. Poppy seeds for this experiment were provided by GB Oseva Pro s r.o., o.z.VÚO Opava a Český mák s.r.o.
Poppy seeds were sown in containers of a size of 7,5x7,5 cm. Used cultivation medium is mixture of garden substrate A with quartz sand in a ratio of 2: 1. Plants were grown under controlled conditions in grow chamber Conviron E8. Before starting the experiment, the temperature was at 10 ° C during day and 5 ° C over night. Light cycle was 13 hours light and 11 hours darkness. The maximum value of irradiance was 800 micromol. The experiment was initiated in phase of rosette develoment. At this developmental stage were the plants exposed to frost temperatures (-5 ° C) over night and chill temperatures (10 ° C) duringthe day. After a week the plants were regenerated for 1 week at 5 ° C over night and 10 C during the day.
All tested plants were in two day intervals measured of proline concentration after exposure to low temperature stress. At the same intervals as proline, were measured the values of relative elektrolyte leakage (Rel%).The obtained results show that the influence of low temperature causes increase of proline level. Control winter-crop variety Zeno exhibited the high kontent of proline (693.33 microg.g -1) in comparison with the spring poppy varieties. After regeneration were afected varieties Lazur, Opal, Buddha, Korneuburger Weisser, Aplaus, Major, Sokol, Zeno reduced the content of proline under the influence of higher temperatures. On the other hand the varieties Ofeus, Tatranský, Opex, Orbis, Maraton, Florián, Marianne and Albin were reversed with an increase of proline level. Proline concentration levels were constant and unchanged in case of varietes Postomi and Akvarel. Values of relative elektrolyte leakage were increased proportionately with length of exposure to low temperatures on the tested plants. The lowest value of membrane damage was after exposure to low temperatures observed in case of variety Zeno (23.53%). In the case of spring varieties was lowest membráně damage estimated in a variety Korneuburger Weisser (44.88%), while the highest was in the variety Orfeus (53.15%). It demonstrated the different responses of individual poppy varieties at low temperature. Most sensitive to low temperature were varieties Orfeus, Postomi, Akvarel, Orbis, Sokol and Marianne. As less sensitive to stress caused by low temperature seems to be spring varieties Opex, Buddha, Korneuburger Weisser, Albín.
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Changes in gas exchange of selected genotypes of poppies depending on the influence by low temperature
Málek, Jan ; Hnilička, František (advisor) ; Orsák, Matyáš (referee)
The Czech republic is one of the biggest Opium poppy producers. In 2015, this plant was grown in an area of approx. 32 650 ha. Since the poppy is usually sown quite soon in the spring, it can be damaged (or negatively affected) by low temperatures. The main objective of this Bachelor thesis was to determine the speed of gas exchange in several poppy species affected by low temperatures.
In this research, altogether 12 various genotypes of Opium poppy (Papaver somniferum L.) were included in (development phase 35 BBCH). These were concretely following poppy species: CM 112, Sokol, Major, Opál, Florián, Albín, Postomi, Marianne, Budha, Lazur, Aplaus and Orbis. Under controlled conditions of a clima-box (Conviron), the plants were exposed (within 1 week) to daily temperatures of 10 °C and night temperatures of -5 °C. After one week, the night temperature was increased to 5 °C, while the daily temperature remained the same (i.e. 10 °C). In following two weeks, there was measured the photosynthesis- and transpiration- speed (using the infrared gas analysator LDpro+SD).
Base on measurements made, there was stated, that there could be noted quite big differences in photosynthesis- and transpiration- speed in particular poppy genotypes involved. This fact proves the existency of obvious differences in gas exchange speed of particular poppy species included in this research. Between the particular measurement terms, there could be also noted differences in photosynthesis- and transpiration speed, which proves the influence of low temperatures on photosynthesis- and transpiration speed.
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Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.)
Slowiaczek, Lukáš ; Hnilička, František (advisor) ; Jan, Jan (referee)
Diploma thesis with the topic: Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.) was worked out at the Department of botanics and plant physiology, Faculty of agrobiology, food and natural resources ČZU in Prague. The purpose of this thesis was to determine genotype and ontogenetic differences in amount of yield, production of dry mass, and value of growth-analytic characteristics in certain species of opium poppy. Diploma thesis was based on the fact that most of the studies about opium poppy (Papaver somniferum L.) are focused on the content of alkaloids and other substances present in poppy seeds. Due to this facts, it is necessary to describe the genotypes of poppy plants and their genetic resources from the physiological point of view.
Following species of opium poppy were chosen for the experiment: Akvarel, Albín, Buddha, Florian, Korneuburger Weisser, Lazur, Major, Marianne, Opál, Postomi, Sokol, Tatranský. These species were chosen according to their content of alkaloids, especially morfine very low content, low content, medium content and high content of morfine. All of the tested genotypes are tested as object of study of genetic poppy resources. Seeds were supplied by GB Oseva Pro s.r.o., o.z. VÚO Opava and company Český mák.
Field experiments were established at the research laboratory FAPPZ ČZU in Prague, in Červený Újezd. This location is in Prague-West division, in area of Middle Bohemia. The altitude of this location is 401 metres above the sea level. The size of the field for testing was 2 m2. The experiment was based in 4 repetitions by the method of Latin square. Winter wheat was used as the preceding crop. Opium poppy was planted according to agrotechnical principles of planting.
Content of dry mass in individual plant organs was measured in certain ontogenetic stages of opium poppy (35, 45, 49, 52, 54, 62, 81 BBCH). Size of leaf area was assessed by the image analysis WinDias. Yield of seeds was assessed as well. Growth-analytic characteristics were calculated from the values of wieght of dry mass and size of leaf area.
The results confirmed that the size of plants is influenced by its ontogeny and also by its species. The results also confirm, that the height of plants is not influenced by the content of morfine. Connection between the colour of seeds and height of plant was not confirmed either. Increase in weight of above-ground biomass depending on ontogenetic stage and influence of plant species was confirmed. There are differences in production of yield in species of poppy with different contents of morfine because the average weight of yield in species with very low content of morfine was 112,36 g, in species with low content of morfine 110,41 g, in species with middle content of morfine 12167 g and in species with high content of morfine 123,16 g. It was approved that ontogeny of plants influences the leaf area index (LAI) as it increases until blooming and then it decreases as a result of senescence and falling off the old leaves. Differences in leaf area index were detected among the species of opium poppy, depending on the content of morfine. It was detected that crop growth rate (CGR), relative crop growth rate (Rw) and relative growth rate of assimilation area (RA) are influenced by the ontogeny of plants. It was detected that species with high content of morfine in poppy seeds (Buddha, Postomi, Lazur) and species with white seeds Sokol and Albín have lower yield than species with middle content of morfine.
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Genetic Diversity of Semi-captive Population of Western Derby Eland (Taurotragus derbianus derbianus) in Senegal and Phylogenetical Relationships between Western Derby Eland (T. d. derbianus) and Eastern Giant Eland (T. d. gigas)
Zemanová, Hana ; Lukešová, Daniela (advisor) ; Jarmila, Jarmila (referee)
Representatives of family Bovidae are subjects of many studies concerning with their phylogeny, phylogeography, time of divergence or genetic diversity. Taxonomy is solved by comparison of morphological characteristics or by genetic approaches, genetic diversity could be solved by pedigree or by genetic analyses too. Tragelaphinae number nine species of two genera, Tragelaphus sp. and Taurotragus sp. The antelopes of the genus Taurotragus (T. derbianus and T. oryx) belong to the largest antelopes of the world. Derby eland (Taurotragus derbianus) has two subspecies, Western Derby eland (T. d. derbianus) and Eastern Giant eland (T. d. gigas), which are distinguished on the basis of morphological characteristics. Western subspecies (T. d. derbianus) is classified as critically endangered. There lives the only population in Niokolo Koba National Park in Senegal, which numbers fewer than 200 individuals. For the conservation, the semi-captive breeding programme has been established in 2000. It was created by six founders (one male and five females), which are presumed to be non-related. The population within this programme had 95 living individuals in 2013, living in seven herds in Bandia and Fathala reserves in Senegal. The population is under breeding management, which efforts to minimize kinship of the individuals. Studbook was established for the Western Derby eland (T. d. derbianus) in 2008 and is published annually. It acts about small population with low number of founders and no gene flow, which is threatened by inbreeding and genetic drift. Genetic diversity of the population was evaluated by means of microsatellite markers and the results were compared with the results of pedigree analysis. Pedigree analysis showed the highest genetic diversity in the generation of founders (FOUNDERS). It decreased in the generation of founders' offspring (OFFSPRING 1; born in season 2007/2008), due to the fact, that the only male took part in the reproduction. And it increased again in the generation of offspring of founders' offspring (OFFSPRING 2; born in the season 2009/2010), because more individuals were included into the reproduction. Fifteen individuals and five polymorphic microsatellite loci (from the total number of 13 tested loci) were chosen for the genetic study. The parameters of genetic diversity (HE and HO, Ar and deviations from Hardy-Weinberg equilibrium, and FIS and FST) were evaluated. Not any deviations from Hardy-Weinberg equilibrium were found out. The results of genetic analysis confirmed the highest genetic diversity in the population of founders (Ar = 2.79; HE = 0.664; HO = 0.750; FIS = --0.154). In both generations of offspring values of allelic richness and observed and expected heterozygosity decreased (Ar = 2.15; HO = 0.580; HE = 0.586 in OFFSPRING 1 and Ar = 2.14; HO = 0.370; HE = 0.480 in OFFSPRING 2). Contrary to the results of pedigree analysis, there was not been observed any improvement in OFFSPRING 2. The resultant values of genetic diversity parameters were quite satisfactory, despite of the low number of founders and mating of related individuals.
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Biotransfer of selected risk metals into plants and their accumulation and distribution in plant organs
Le Minh, Phuong ; Lachman, Jaromír (advisor)
Contamination of soils with heavy metals is one of the serious environmental problems threatening human being. Heavy metals are considered as the special hazard of soil pollutants because of the adverse effects on the plant growth, the amount, activity of useful microorganisms in soils and the quality of food. Regard to the persistent and toxicity, the heavy metals are toxic when we consider different kinds of pollutants in soils. In the soil, zinc (Zn), cadmium (Cd), lead (Pb) and mercury (Hg) toxicities frequently occur than the other metals because of their precipitation and sorption by the soil. It is a very dangerous situation because when these metals are taken up by plants, they can be transported to the food web and food chains.
In the present study, the accumulation of four heavy metals (mercury, zinc, lead and cadmium) in the whole grain of spring accessions of emmer, einkorn and common spring wheat cultivars and potato (Solanum tuberosum) is reported. Heavy and essential elements were monitored in potato cultivars in the exact field experiments and in hydroponically grown plants. The elements were determined by methods FAAS, ET AAS, and AMA (Advance Mercury Analysis). Statistical analyses were performed using SPSS 9.0 with the Tukey HSD (Honestly Significant Difference) test (alpha equal to 0.05).
In our study, the concentration of heavy metals decreased in the order zinc (Zn) > lead (Pb) > cadmium (Cd) > mercury (Hg) in the wheat grain. The comparison between three varieties of investigated wheat revealed that the emmer wheat was rich in zinc content (62.12 mg kg-1 dry matter), while the spring wheat had the lowest average concentration of zinc in the grain (40.99 mg kg-1 dry matter). Generally, the values of lead concentration in grain wheat varieties were low (ranging from 0.1268 mg kg-1 dry matter to 0.2950 mg kg-1 dry matter).
The concentrations of mercury in four typical growth stages of wheat (boot stage 10, heading stage 10.2 1/4 of head emerged, leaf-stage 10.2 and stage ripening 11 according to Feekes) were also determined. It has been shown that the concentrations of mercury in different wheat varieties were absorbed differently at different growth stages of plant. Stage 10.2 and leaf stage 10.2 showed the high mercury content (0.0152 mg kg-1 dry matter and 0.0214 mg kg-1 dry matter, respectively). Among individual varieties significant differences were determined.
Amounts of toxic and potentially toxic elements detected in investigated potato tubers are characterized by a large variability within investigated groups. Performing statistical analysis (one way ANOVA) showed that there were no significant differences between two investigated groups of samples (samples from Uhříněves and Valečov in the year 2013 and 2014) considering either one of investigated metals. Measurable levels of mercury were found in smallest amounts in all investigated potato samples comparing to other metals (Cd, Pb).
Plant cells compared to animal cells are characterized by the formation of cell walls. Plasma membrane or cell membrane is a biological active membrane separating the interior of cell from the outside environment. An adjusted method for isolation of protoplasts was developed and adapted for isolation of protoplasts from plant material (potatoes). In our experiment, the plants were grown hydroponically in the Research Institute of Plant Crops Prague-Ruzyně. If we examine the plant membrane, one option is to remove the cell wall by means of special mixture enzymes. Protoplasts were released in the dark at 25 degrees of Celsius for 18 hours. The 70 and 90 microns sieve was used to filter and then centrifugation for 5 minutes at 100 x g. All the steps were carefully carried out to prevent the damage or breakage of protoplasts.
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Testing of bioeffectors on phosphorus mobility in soils
Holečková, Zlata ; Balík, Jiří (advisor)
Crave for increase in agricultural production over the past sixty years reflects in widespread use of mineral fertilizers. Due to the growth of the world population an increase of overall demand for feed and food is expected. Limited availability of cultivatable soil and increasing dependance on mineral fertilizers can be also anticipated. It is therefore important to find alternative strategies for plant nutrition. In 2012, the project of cooperation between several universities in Europe was revealed. This project examines the use of bioeffectors in crop production. Use of these substances should help to reduce the input of mineral fertilizers used in agriculture and improve land usage. Thesis will perform testing in real conditions of different geographic locations. The main objective is to develop new approaches to the use of so-called bioeffectors based on the mechanism of action of living microorganisms and active natural substances. Bioeffectors may be, depending on soil and climatic conditions, a key factor for overcoming limitations in the availability of nutrients. They can contain microorganisms (bacteria, fungi) and active natural compounds (digest from soil or compost, microbial residues, plant extracts, metabolites from biological processes, dried herb material or seaweed). These substances are developed for a wide variety of crops (e.g. corn, wheat and tomatoes). Their effectiveness consists in nutrients mobilization from less accessible forms in the soil. The thesis is mainly focused on the impact of bioeffectors on various forms of phosphorus in the soil. Phosphorus in the soil belongs to essential elements necessary for the growth and development of plants. Despite its necessity in plant metabolism is its content in the soil relatively low. A higher phosphorus content is in most samples of soil mainly in the surface area because an increased biological activity occurs there causing the accumulation of organic material. Some influence can be also observed in connection with application of organic and mineral fertilizers. Amount of phosphorus in soil may vary depending on parental rock type, texture and land management (the ratio of P supplied and type and method of land cultivation). Above mentioned influences even have an effect on relative amount of organic and inorganic forms of phosphorus. Three basic groups of phosphorus contained in the soil can be described: inorganic phosphorus compounds, organically bound phosphorus and exchangeable absorbed phosphorus. The amount of phosphorus bound in the fractions depends mainly on the time of fertilizer application, including the impact of earlier interventions. For these reasons, it is necessary to execute detailed analysis of the various forms of phosphorus in the soil after application of mineral fertilizers together with bioeffectors. In this dissertation variety of pot and field experiments will be set up, where samples from each variation of soil and plant will be separated and researched. Samples will be analyzed and amounts of phosphorus will be measured.
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Černý, David ; Malaťák, Jan (advisor) ; Mašek, Jiří (referee)
Organic wastes represent available replacement for crops grown specifically for biofuel production. However, the combustion of solid biofuels is often accompanied by technical problems. Biofuels made of organic wastes require the source of which do not contain hazardous substances that could be their combustion in simple combustion sources came into the atmosphere as emissions.
This thesis is focused on the assessment of technical and operational parameters of small combustion plants while burning biofuels and also of the measurement of emission concentrations of CO and NOX in the flue gas and its subsequent analysis. Samples were selected as solid fuel in the form of a lump, briquettes and pellets made from advanced materials. Incineration was realized in the fireplace with a grate furnace and automatic stove.
The bark briquettes could appears to be a suitable substitution for coal or wooden briquettes, and during combustion in grate emission concentrations of CO were achieved with a value of 560.8 +/- 98.9 mg.m-3,on the contrary NOX levels were relatively high
1 193.6 +/- 141.7 mg.m-3. In case of this work, when using the furnace the briquettes were made from compost , whose emission levels are higher: CO 1 487.8 +/- 418.8 mg.m-3, NOx levels were somewhat lower 330.8 +/- 26.3 mg.m-3 than the briquettes bark.
An experimental sample of sewage sludge, which has been modified by hydrothermal carbonisation, was burned on the grate furnace and the measured emission levels for CO
1 493.3 +/- 237.3 mg.m-3 and NOX levels 544.8 +/- 20.2 mg.m-3, positive because it was achieved the rated output of the combustion equipment and the heat of combustion of the sample is very low 12.61 MJ.kg-1.
The most efficient combustion of 80.8 +/- 2,7 % was achieved using automatic stove, during burning waste wood pellets certificate ENplus A1, the very low levels of emission and a very low emission levels for CO with a value of 117.5 +/- 14.4 mg.m-3 and values NOX
110.3 +/- 2.9 mg.m-3 were measured. Following experiments on this device were intended to increase the efficiency of a combustion plant by installing a hot-air heat exchanger and preheating combustion air. Method with preheating combustion air caused an increase in the emission concentration of CO.
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Evaluation of cow fertility on profitability of milk production
Čítková, Dana ; Stádník, Luděk (advisor) ; Dalibor, Dalibor (referee)
Objectives of this study were to evaluate effects of milk production level and calving interval, resp. days open period length on the lactation curve parameters and milk production profitability. Data were obtained from Holstein dairy farm. From our results can be stated, that cows with the longest days open period length reached significantly the highest lactation persistence. Due to their ability to maintain high milk productivity after peak day, average daily milk yield was not in these cows, despite long lactation, lower than in cows with shorter lactations. From the economic point of view, milk production profitability with calving interval length decreased and with milk production level increased. Low profitability in cows with long days open period caused high replacement and fertility costs. Feeding costs despite expectations didn´t differ between these groups. All early lactating cows are fed with the same expensive concentrated feeding ration, thats why there is not possible to save feeding costs in high persistent cows with low milk production increase after calving
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Factors influencing seed germination, emergence and mortality in soil seed bank of Bromus sterilis L.
Žďárková, Veronika ; Soukup, Josef (advisor)
Bromus sterilis L. (barren brome) spreads rapidly in many European regions. In the Czech Republic, its importance has increased dramatically over the past 10 years. Barren brome is reported as a problem weed in winter crops such as winter wheat, winter barley and oil seed rape, in vineyards and in other cultivated places. Barren brome has been becoming troublesome weed of winter cereals mainly in reduced soil tillage systems. The factors, that are important for its spreading and adaptability under different environmental conditions, are dormancy and germination. Optimal timing for seed germination varies with respect to natural conditions and it is determining for plant development. These traits are adapted to different conditions and habitats; therefore the dormancy and germination response patterns to those conditions vary significantly. Recently there are not many information available on germination behaviour, therefore this study was focused on seeds of barren brome and its characteristics, which were collected in different regions of the Czech Republic. Seeds were investigated under different temperatures, light regimes and water stress in a wide range of conditions. The following characteristics of seeds were studied the dormancy, the dynamics of germination, the temperature optimum, the age of seeds and dynamics of emergence from different depths and persistence in the soil profile under field conditions. Dormancy and germination are influenced by external conditions. The dormancy of seeds barren brome is very short or missing. The germination is influenced by light; the seeds germinated better under darkness than under light regime. The primary dormancy of B. sterilis was short and the seeds needed only three weeks for after-ripening. The seeds of B. sterilis showed broad ecological valence to hydrothermal factors germinating in the wide range of 3 to 35 °C. The temperature optimum is 5-23°C. The germination was only slightly influenced in an environment with low water potential. Germination was limited under water stress only at lower temperatures under 10°C. The response to light at various temperatures showed that seeds germinated better in darkness in all temperatures regimes, than in alternating light regime, especially at lower temperatures. The emergence declined significantly with burial depth (under 40 mm). The seeds were not able to survive in the soil seed bank for a longer time and fall seeds lost viability after 1 year burial in soil profile. These results may be of value for development of predictive models and understanding period when weed control may be most feasible.
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Socially Excluded Localities and Their Revitalization. Case Study: Ústecký Region.
Zoubková, Věra Thea ; Maier, Karel (advisor) ; Jakub, Jakub (referee)
The dissertation deals with the incidence and revitalization of socially excluded localities in a defined study area. The aim of this contribution is to identify and visualize the situation of the Ústecký Region in order to find common patterns of urban deprivation occurrence and to establish the guiding principles of revitalization policies in the Czech Republic. The research took place from September 2010 to June 2015. It combined qualitative and quantitative methods, in-situ and desk research. An analysis of primary and secondary data revealed 130 excluded localities in 42 towns and cities out of 354 in the region. The majority of deprived localities (87 %) can be found in highly urbanized areas. Two thirds of localities are small areas with up to 100 inhabitants. Socially excluded areas emerge in the centre, residential districts as well as on the outskirts of towns; however, three fifths of localities are not spatially segregated. Prefabricated housing estates are the largest and most populated deprived areas. Every other inhabitant of socially excluded localities in the Region lives in a prefabricated house. Brick houses are the most proliferated type of housing facility used as accommodation for socially excluded people. They can be found in every other deprived area. Vast majority of them was built before 1989. Family houses are deprived very scarcely; however, they belong to the most dilapidated part of socially excluded housing estate. Two thirds of localities have developed naturally, while the other third has been created by a regulated relocation of socially excluded people. Vast majority of deprived areas have existed for more than 5 years. Private property prevails. Half of the localities have entered the process of revitalization to improve the urban environment, human resources and security. Applied policies aim to keep the inhabitants in place and improve the quality of public space and service. However, involvement of residents in local regeneration policymaking is weak and ineffective. The renewal process depends on grants and subsidies. Europeanization is strong especially in big cities; nevertheless, most localities are small and therefore don't qualify for the sources of the built environment programs which prefer large areas of intervention. Results of revitalization process differ from one locality to another. Areas of housing and employment fall behind in particular. As far as property regeneration is concerned, the best owners are municipalities, house unit owners associations and housing associations. Not in one case, however, reaches the share of regenerated housing units 50 % of deprived properties. In any case, the market prices of local flats stay low compared with those of correspondingly equipped properties elsewhere in town.
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