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Effects of single beta-alanine dose on performance at submaximal load
Šimsa, Jáchym ; Sýkora, Karel (advisor) ; Šteffl, Michal (referee)
Title: Effects of single beta-alanine dose on performance at submaximal load Objectives: The aim of this study was to investigate the effects of single beta-alanine dose on blood-lactate level in capilary blood and whether it will affect the speed of passing the test. Methods: A double-blinded cross-over study conducted on 16 male students of Military Department at the Faculty of Physical Education and Sports of Charles University (mean ± SD: weight 79,31 ± 6,97 kg; height 181,56 ± 6,36 cm; age 21,63 ± 1,45 yrs). The study was performed in Military Sport Centre Pilotů 217/12, 161 00, Prague. Each volunteer underwent two performance tests after consuming either placebo (maltodextrine) or beta-alanine (50 mg/kg). Time needed to complete the test and blood-lactate concentration before and during 5th and 10th minute after completion was measured. Volunteers also filled a short questionnarie regarding subjectively percieved load and side effects, and whether they were administered beta-alanine (BA) or placebo (PLA). For verification of normal distribution were used Q-Q Plots and statistical significance of BA use was determined using student's t-test. Results: Measured lactate concentrations were (mean ± SD; mmol/l) for BA in min 0 = 1,29 ± 0,37; min 5 = 11,48 ± 1,45; min 10 = 10,64 ± 1,76; PLA in min 0...
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Utilization of nutritional supplements at elite athletes
Teclová, Julie ; Coufalová, Klára (advisor) ; Jandová, Tereza (referee)
Title: Utilization of nutritional supplements at elite athletes Objectives: The aim of the bachelor's thesis was to determine the character and current trends in the use of nutritional supplements by the top athletes in various sports. The partial goal was to compare the results of the use of nutritional supplements and to develop feedback for individual companies selling sports supplements. Methods: A questionnaire survey was used to collect data, containing open and closed questions regarding the use of nutritional supplements. It also contained questions in what form athletes use supplements, the main reasons for use, at what frequency and what are the preferred brands of supplements. The survey was sent in electronic form to 33 respondents who practice top-level sports in the Czech Republic. Results: It was found that all the surveyed individuals take nutritional supplements. By the endurance athletes, the claim that they prefer supplements with an excitatory effect in combination with supplements leading to weight reduction has been confirmed. It was also proven that more than 50 % of respondents prefer a particular brand of nutritional supplements and do not plan to change it, and more than 50 % of athletes surveyed use supplementation daily. It was also found that elite athletes prefer the...
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The effect of citrulline and citrulline malate supplementation on selected physiological indicators - review study
Richter, Michael ; Šteffl, Michal (advisor) ; Kinkorová, Ivana (referee)
Title: The effect of citrulline and citrulline malate supplementation on selected physiological indicators - review study. Objectives: Systematic findings overview of the effect of citrulline and citrulline malate on healthy individual's locomotor system and other physiological indicators. Methods: The study is designed as a findings overview. Electronic databases pubmed.com and ukaz.cuni.cz were used as an information source. "Citrulline" or "citrulline malate" and "exercise performance" and "randomized controlled trial" keywords were used for the information search. Results: Eighteen studies and 307 individuals are included in the overview. Studies are categorized according to duration and type of supplementation substance. Four studies agree on the strength improvement after acute use of citrulline malate. Four studies agree on endurance improvement in case of citrulline long term use. Three studies conclude on pain mitigation in case of substance use 24, 48 and 72 hours after training. Also, studies agree on increase of the levels of citrulline, arginine and ornithine plasmatic concentration after use of citrulline and citrulline malate. Three studies indicate possible fatigue decrease during the training in case of use of citrulline. Conclusion: Use of citrulline and citrulline malate can be...
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Optimal conditions of caffeine use and its effect on sports performance
Šťastný, Daniel ; Pavelka, Radim (advisor) ; Petr, Miroslav (referee)
Title: Optimal conditions of caffeine use and its effect on sports performance Objectives: Based on current scientific knowledge, summarize the effect of caffeine on various aspects of sports performance, and also to determine the optimal conditions for the use of this substance to maximize the ergogenic effect. Methods: Systematic literary research Results: The ideal dose of caffeine to improve athletic performance is in the range of 3- 6 mg/kg, and most preferably in the form of tablets 60 minutes before exercise. This substance has been shown to improve aerobic endurance performance, sprint speed and cognitive function such as accuracy and attention in athletes. Caffeine in trained individuals may also improve anaerobic endurance, maximum and endurance strength, simple reaction time and other cognitive functions, but further studies are needed. . Keywords: sport, supplementation, aspects of performance, conditions of use, stimulation, alkaloids
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Biofilm formation in probiotic cultures and its application in pharmacy
Ryšávka, Petr ; Obruča, Stanislav (referee) ; Vorlová, Lenka (referee) ; Márová, Ivana (advisor)
The work was comprehensively focused on the development of adhesive forms of probiotics in the form of a biofilm on combined carriers with a prebiotic component. The second part dealed with the influence of food on the multiplication and survival of selected types of probiotic bacteria. Subsequently, the effect of individualized probiotic supplements on changes in the human intestinal microbiome was monitored. Suitable adherent probiotic strains for biofilm formation were selected and tested. Methods have been introduced and different variants of carriers for culturing and binding bacteria have been tested. In vitro experiments verified the stability of biofilm stucture and its resistance to low pH, bile and antibiotics in comparison with the planktonic cell form. The antimicrobial effect of probiotic strains in the form of a biofilm was studied. The cultivation of the multispecies biofilm on the combined carrier was optimized and the stability of the biofilm and the final viability of probiotic bacteria were confirmed. Furthermore, the influence of various foods and beverages on the viability of probiotic bacteria was evaluated with emphasis on the simulation of passage through the gastrointestinal tract. Both models, solutions with standardised concentrations of alcohol, sugar, salts, proteins or different pH and different types of real foods and beverages were tested. The effect of food and beverages was tested on monocultures of Lactobacillus acidophilus, Bifidobacterium breve and on probiotic capsules containing a mixed culture of probiotic microorganisms. The survival of probiotics in various food matrices in the simulated gastrointestinal tract was quantitatively different. We managed to define foods suitable for supporting the multiplication of probiotic bacteria. A separate part of the work was focused on the targeted modulation of the intestinal microbiome by individualized probiotics that were prepared on the basis of molecular biological analyzes of the intestinal microbiome aimed at detecting the percentage of lactobacilli, bifidobacteria and phylum Firmicutes and Bacteroidetes. Personalized probiotic supplementation confirmed the positive effect of this approach on microbiome changes, especially on the increase of the content of lactobacilli, bifidobacteria and the overall diversity of the microbiome.
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A systematic overview of the recommended amount of protein in elite sports and its reflection in the vegan diet
Vondrysová, Kristýna ; Bunc, Václav (advisor) ; Coufalová, Klára (referee)
Title: A systematic overview of the recommended amount of protein in elite sports and its reflection in the vegan diet Goals: The aim of this work will be to identify whether there are vegan elite athletes who have participated in national / international competitions in sports such as weightlifting, strongman, powerlifting and placed third. Compare the individual recommendations for the volume of protein in the diet based on the vegan diet, and in this sense compare recommendations for the use of the vegan diet in elite sports. Method: This work is processed in the form of an overview study. The search for analysis was selected using the keywords: vegan elite athletes, vegan nutrition, elite sports nutrition, protein nutrition, strength sports. A total of 1077 studies formed the basis for the narrower selection of a total of 12 suitable studies for mutual comparison. The basic method is meta- analysis. Results: The average recommended values for protein intake are 1.8 ± 0.3 g / kg body weight. A total of 66% of the 12 analyzed studies agreed with the statement that vegan nutrition offers opportunities for the full representation of the protein component in the diet and thus creates the necessary prerequisites for achieving high sports performance. However, careful comprehensive diet design is...
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Effects of single beta-alanine dose on performance at submaximal load
Šimsa, Jáchym ; Sýkora, Karel (advisor) ; Šteffl, Michal (referee)
Title: Effects of single beta-alanine dose on performance at submaximal load Objectives: The aim of this study was to investigate the effects of single beta-alanine dose on blood-lactate level in capilary blood and whether it will affect the speed of passing the test. Methods: A double-blinded cross-over study conducted on 16 male students of Military Department at the Faculty of Physical Education and Sports of Charles University (mean ± SD: weight 79,31 ± 6,97 kg; height 181,56 ± 6,36 cm; age 21,63 ± 1,45 yrs). The study was performed in Military Sport Centre Pilotů 217/12, 161 00, Prague. Each volunteer underwent two performance tests after consuming either placebo (maltodextrine) or beta-alanine (50 mg/kg). Time needed to complete the test and blood-lactate concentration before and during 5th and 10th minute after completion was measured. Volunteers also filled a short questionnarie regarding subjectively percieved load and side effects, and whether they were administered beta-alanine (BA) or placebo (PLA). For verification of normal distribution were used Q-Q Plots and statistical significance of BA use was determined using student's t-test. Results: Measured lactate concentrations were (mean ± SD; mmol/l) for BA in min 0 = 1,29 ± 0,37; min 5 = 11,48 ± 1,45; min 10 = 10,64 ± 1,76; PLA in min 0...
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Changes in Bone and Mineral Metabolism and the Role of Vitamin D in Very Low Birth Weight Infants
Matějek, Tomáš ; Skálová, Sylva (advisor) ; Šumník, Zdeněk (referee) ; Kutílek, Štěpán (referee)
Changes in bone and mineral metabolism and the role of vitamin D in very low birth weight infants. Firstly, the aim of dissertation work was to estimate physiological parathyroid hormone (PTH) levels and their relationship with bone metabolism parameters in otherwise healthy preterm newborns with birth weight 1000-1500 g. Secondly, to evaluate vitamin D status in mothers and their very low birth weight infants (VLBW) at birth and at discharge with currently recommended supplementation of vitamin D. Thirdly, to compare clinical outcomes of VLBW infants with 25-hydroxy vitamin D [25(OH)D] levels ≤ and > 25 nmol/l in umbilical cord blood and finally to evaluate umbilical cord vitamin D as a risk factor for respiratory distress syndrome in preterm infants. It is a set of prospective observational studies involving immature newborns with birth weight below 1500 g. The parameters of mineral and bone metabolism were analysed in umbilical cord blood and newborn serum and urine during hospitalisation (PTH, 25-hydroxy vitamin D, S-Ca, S-P, ALP, U-Ca, U-P) and in pregnant women before delivery (25-hydroxy vitamin D). Bone mineralization was evaluated by bone densitometry. In a pilot study, from the total 134 examined serum samples for PTH levels the estimated reference range was 1.6 - 9.3 pmol/l. From the...
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Serum concentration of vitamin D in patients with femoral neck fracture
Matoušková, Karolína ; Wenchich, László (advisor) ; Zikán, Vít (referee)
Vitamin D significantly affects metabolism of the bone tissue. It keeps homeostasis of calcium and phosphor and thus contributes to healthier mineralization of bones. Lack of those substances pertains to risk factors for development of osteoporosis and subsequent complications during possible downfalls and fractures. The role of vitamin D in bone disease has been increasing in recent years, but its importance in the early post-fracture period is not yet fully understood. This thesis is a prospective study of metabolism of vitamin D and its metabolites in post-operative stage of patients with femoral neck fractures. It is a basic quantitative research, where received data are analysed. Blood samples obtained from a total of 18 patients, whose blood was taken on the day of surgery and 6 weeks after surgery (± 1 week), were used as material. Resulted values of 25-OH vitamin D increased from 23,02 ± 16,02 nmol/l to 25,75 ± 16,30 nmol/l (diameter ± standard deviation). In contrast, 1,25-(OH)2 vitamin D decreased from 76,81 ± 41,84 pmol/l to 55,88 ± 26,34 pmol/l. Statistically significant results at a significance level of p≤0,05 were only observed for parathyroid hormone (PTH), with values decreasing from 6,31 ± 3,95 pmol/l to 3,96 ± 2,25 pmol/l. Vitamin D examinations in our patients showed reduced...
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Effect of selected dietary supplements on the nutritional status of athletes
BOČKOVÁ, Dominika
A modern world brings a various poitns of views and possibilities in human nutrition. This theses is focused on dietary supplements and its using by athletes. Especially it is focused to the athletes for who the sport is essential part of life. The main aim is to chart supplements which are the most commonly used by athletes, to chart their features and functionality, eventualy riskness. Finally the secondary aim is to find out the sense of using them by the subjective view of athletes. What is the real need of using supplements? Is it necessary in in the context of preventing a nutritional deficit? Does it bring any benefits or is it just a marketing movement? Theoretical part is focused to the most using supplements, the legislation and division. Supplements will be analyzed by their functionality and effects. Practical part is focused on athletes for who is using supplements most often - enthusiasts of fitness-bodybuilding and people who often visit gym. The frequency was given by this criterion - it was focused on athletes who have visited the gym several times a week in the last year or longer. Research was conducted by questionnaire and expanded by interview if it was necessary. The questionnaire was distributed in printed version.For realisation was determined a quality research which was attended by ten respondents. All of them operate in sport at amateur level, some of them take part in amateur races, for the rest of them is bodybuilding just a hobby. The questions were asked to find out the way of using of supplements - their frequency and variosity.The research shows the differences between men and women, between profesional athletes and hobby athletes, their knowledge about supplements and the influance of sport enviroment. The athletes were questioned where they found the information about the supplements, how much they invest to buying it and why they using them. For processing purpose was used qualitative analyse of questionnaire. It was discovered that most of respondents do not observe recommended daily dosing, in most of cases was the main reason the recommendation by friend without the consultation with specialist so the athlet did not know what is the real need of nutrition and if the using of supplements is necessary. The most frequently used were the protein drinks and micronutrients - vitamins and minerals, especially vitamin C, magnesium and zinc, and omega 3 fatty acids. Theses could be used like an information resource for explanation what is the point of supplement, the sense of using them in connection of main aim of sport effect. In could also give an information for these ones who are interest in sport nutrition and the general public.
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