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Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. II
Kohelová, Eliška ; Cahlíková, Lucie (advisor) ; Siatka, Tomáš (referee)
Kohelová E: Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. II Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017, 77p. The aim of the diploma thesis was a preparation of alkaloidal extract from fresh bulbs of Narcissus cv. PROFESSOR EINSTEIN to izolate two Amaryllidaceae alkaloids by column and thin layer chromatography. Subsequently these alkaloids were subjected to structural MS and NMR analysis and tested for biological activity against human cholinesterases (HuAChE and HuBuChE) and for cytotoxic activity. In cooperation with the Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, antifungal and antimicrobial activities of alkaloids were tested, and in cooperation with Faculdade de Medicina da Universidade de Lisboa Portugal antimalarial activity of alkaloids was studied. Isolated substances were identified as galanthamine, caranine and pluviine. Pluviine was isolated in an amount allowing only structural analysis. In the test for determining cholinesterase inhibitory activity of caranine the following values were obtained: IC50, HuAChE = 320 ± 42 μM a IC50, HuBuChE = 486 ± 56 μM. Galanthamine (IC50,...
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Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. I
Burkertová, Alice ; Cahlíková, Lucie (advisor) ; Hošťálková, Anna (referee)
Dohnalova A: Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. I; Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017, 64 p. The Amaryllidaceae family includes bulbous, beautifully flowering plants that have been used for thousands of years in traditional medicine. The major chemical compounds found in this particular plant family are alkaloids, namely Amaryllidaceae alkaloids. Until now, more than 500 isoquinoline alkaloids have been discovered, which exhibit a diverse biological activity including antitumor and antibacterial. They are also able to inhibit acetylcholinesterase. The aim of the diploma thesis was to process 34 kg of fresh bulbs of Narcissus cv. PROFESSOR EINSTEIN and to prepare an alkaloidal extract. This extract was further divided by column chromatography to almost 500 fractions which were merged based on TLC into 27 subfractions. The subfractione No. 17 was selected for isolation of at least one pure alkaloid. Preparative TLC was used for the the isolation. One pure compound was obtained in crystalic form which was then subjected to structural analysis by EI-MS and NMR methods. Further studies of biological activities were performed in...
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Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. I
Dohnalová, Alice ; Cahlíková, Lucie (advisor) ; Hošťálková, Anna (referee)
Dohnalova A: Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. I; Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017, 64 p. The Amaryllidaceae family includes bulbous, beautifully flowering plants that have been used for thousands of years in traditional medicine. The major chemical compounds found in this particular plant family are alkaloids, namely Amaryllidaceae alkaloids. Until now, more than 500 isoquinoline alkaloids have been discovered, which exhibit a diverse biological activity including antitumor and antibacterial. They are also able to inhibit acetylcholinesterase. The aim of the diploma thesis was to process 34 kg of fresh bulbs of Narcissus cv. PROFESSOR EINSTEIN and to prepare an alkaloidal extract. This extract was further divided by column chromatography to almost 500 fractions which were merged based on TLC into 27 subfractions. The subfractione No. 17 was selected for isolation of at least one pure alkaloid. Preparative TLC was used for the the isolation. One pure compound was obtained in crystalic form which was then subjected to structural analysis by EI-MS and NMR methods. Further studies of biological activities were performed in...
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Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. III
Hanusová, Petra ; Cahlíková, Lucie (advisor) ; Chlebek, Jakub (referee)
Hanusová P.: Alkaloids of Amaryllidaceae family: isolation, structural identification, biological activity. III. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017. For phytochemical study was selected plant cultivar Narcissus cv. PROFESSOR EINSTEIN. The aim of the work was to isolate Amaryllidaceae alkaloids in pure form and screening of their biological activities. Summary alkaloidal extract was prepared from 34 kg of fresh bulbs and separated by column chromatography. Preparative TLC and crystallization were used for the isolation of substances from subfraction 6/1. Two pure alkaloids of galanthamine structural type were obtained. The alkaloids were identified based on their MS, NMR analysis and optical rotation as narwedine and lycoraminone. Lycoraminone has been isolated for the first time from natural source. Both substances were tested for their acetylcholinesterase, butyrylcholinestease and prolyl oligopeptidase inhibition activity. Anticancer activity against two gastrointestinal cancer cell lines Caco-2 and HT-29 (colorectal adenocarcinoma) and antimalarial activity has been also measured. Unfortunately, none of isolated alkaloids showed some significant activity in biological assays. Keywords:...
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The eye of a poet: Antique figures in the work of Jean Cocteau
Wittlichová, Julie ; Jirsa, Tomáš (advisor) ; Hrdlička, Josef (referee)
The thesis is concerned with the instances of the figures of mythology of the antiquity in the works of Jean Cocteau and their application. It focuses on the character of Narcissus, which is examined in connection to Oedipus and Orpheus, while concentrating mainly on the topic of mirroring and the related motives of the eye and sight. This mirroring is then not examined only on the level of an aesthetic object, but also in relation to the recipient and in mutual interferences between various media. The thesis is methodologically based on selected philosophical theses by Maurice Merlau-Ponty and the theoretical concepts of Pierre Brunel, Mieke Bal and Mary Ann Caws. Within the works of Jean Cocteau the thesis is primarily concerned with the analysis of the novels The Holy Terrors (Les Enfants Terribles), Le Livre Blanc and their illustrations, the poem "Tombeau de Narcisse", the play La Machine infernale and the films Blood of a Poet, Orpheus and The Testament of Orpheus.
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Back to the Tribe's Womb: Tendencies in Contemporary Culture
Dvořák, Jan ; Činátlová, Blanka (advisor) ; Bílek, Petr (referee)
Michel Maffesoli and Zygmunt Bauman add the traits of Benjamin's flâneur to the (neo-)nomad, namely his "flâneur" gaze and his relation to commodities. But in the concept of nomad these traits gain specific nature - on the field of fashion they transform nomad into migrant, who is capable of creative work with vanitas. Nomad as a travelling flâneur is a stranger-guest and becomes a tourist, willingly getting lost in the city and voluntarily being surprised by unexpected encounters. Tourists relation to his memories could be described with Benjamin's description of mémoire involontaire as a revived punctum. A tourist prepares his memories like a nicely descending ruins. Souvenir is his materialised memory. It's not only a duplicated plastic Eiffel tower, but a magic artifact. It's a collective aura, what's on Benjamin's mind when he writes about aura regression. The private aura comes instead, turning things to talismans, reenchanting the world. The era of postmechanical reproduction reminds one, that there is a way to reproduce not just mechanically but biologically: a bricolage remix is made. Souvenirs descend and take shape of hommogenic rummage which reveals the fundamental form of postmodern metamorphosis: recyclation. This metamorphosis can finally be used when analyzing the settler turn into nomad...
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Devil Guardian: The Theme of Identity in Mexican Novel on the break of the Millenium.
Alon, Andrea ; Vydrová, Hedvika (advisor) ; Housková, Anna (referee) ; Riebová, Markéta (referee)
The theme of the dissertation submitted is the novel Devil Guardian, whose author is a Mexican writer Xavier Velasco. This dissertation is the very first theoretical work in the Czech environment, devoted to the above-mentioned piece of literature published in 2004. In a sense, Devil Guardian represents a characteristic Mexican novel of the early 21st century, combining tradition and novelty in a surprising and original manner. A significant feature of the contemporary Mexican literature is a departure from the theme of Mexicanity, generally from a programmatic indulging in so-called national literature. Velasco's novel is an exemplary, however, not only piece of literature proving that the literary break-up with Mexico is neither an exclusive nor a dominant attribute of the contemporary Mexican fiction. In Devil Guardian Velasco focuses his mind on the theme of personal and national identity, which he treats in a considerably nontraditional manner, giving an ironical turn to speak to a hypermodern girl moving in the globalized world. The dissertation is divided into six parts. The first part "Originality Rooted in Tradition" refers to Devil Guardian ensuing the tradition of Mexican novel and innovating it. The second part "Xavier Velasco" briefly introduces the author's life and work. The issues...
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