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Slithy, a blend of lithe and slimy
Šillerová, Kateřina ; Alster, Darina (referee) ; Hládeková, Katarína (advisor)
For my work Slithy, a Blend of Lithe and Slimy, a thought that consciousness appears even on the lowest levels of existence is essencial, I create an enviroment of a lab, in which spectator is in the role of another organism, surrounded by other organisms. I'm interested in relationship between microcosmos and macrocosmos and in seeing the same pattern through reality.
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Localization system making use of GPS and GSM
Klozar, Lukáš ; Pust, Radim (referee) ; Šebela, Radek (advisor)
Aim of this master´s project is to analyse localization possibilities in GSM and GPS wireless networks. Choise of proper module and design location device for both GPS and GSM networks. Design and construction of system, which is able to designate it´s positron, using both GPS and GSM networks. The location informations, are send to Internet server, using GPRS system. Localization techniques in both wireless network are described. The system which is able to get position information was designed and constructed. The module´s driving software and the software, which process the localization informations and controls the database storing process, was designed.
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Modelling of Cell Colony Dynamics
Bělehrádek, Stanislav ; Škutková, Helena (referee) ; Sedlář, Karel (advisor)
The content of the thesis is a description of intracellular processes responsible for cell cycle regulation and reactions of cells to external and internal stimuli. Thoroughly described are important signaling pathways with appropriate methods, which can be used to simulate them in silico. From these cellular processes, a cell cycle model is created and implemented in a tool programmed in C ++ with OpenGL used for visualization. The model is then tested for various cell processes including HeLa cells growth. Finally, the results are compared with the behavior of living cells.
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Control and monitoring of parameters in microfluidic systems
Meloun, Jan ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
This thesis discusses the construction of Microfluidic systems for a microscope. The theoretical part of this thesis deals with cell and its survival in the environment. Furthermore, light, light’s behavior and optical spectral analysis in some methods is discussed. Also, the use of the development platform Arduino with wireless data transfer for data visualization is mentioned. Methods for measuring pH are mentioned as well. The practical part is dedicated to the construction of the Microfluidic system with the use of Arduino, smart mobile devices and a developed mobile application including controlling liquid flow with the measured data visualization.
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The effect of therapeutical ultrasound and metalic nanoparticles on carcinoma cells
Kalužová, Adéla ; Rozman, Jiří (referee) ; Bernard, Vladan (advisor)
This thesis focuses on the research of an effect of ultrasound and nanoparticles on cancer cells. In the literature search there the applications of ultrasound field in the interaction with aplication of metallic nanoparticles to viability of cancer cells are described, on the basis of in vitro experiments‘ performance. The purpose of metallic nanoparticles‘ application should be a possible reinforcing of its antitumor effect thanks to the presence of ultrasound field. In next part of the literature search there are included the technical performance of devices, their indications and contraindications, parameters of ultrasound fields and application of metallic nanoparticles. The metallic nanoparticles have been becoming a part of the human’s life more and more, that is why it’s necessary to deal with these miniature particles at a higher level. The aim of this thesis is to suggest the ways of resolution based on the principle of sounding of cells with the ultrasound field of suitable parameters and in combination with the application of metallic nanoparticles with a successive performance of the cell viability tests. The viability was determinated by the MTT assay test. Another part of this work is the measurement of parameters of this zone by means of a submersible needle hydrophone, for a better understanding of the ultrasonic zone. In the final part of this work there is necessary the statistical evaluation of the measured data of the experiments carried out and the discussion of the results obtained.
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Jaroslavice – place in the landscape
Šmejkal, Jiří ; Galeová, Nicol (referee) ; Ponešová, Barbora (advisor)
The theme of this diploma thesis is the architectural study of the complex of the Farm of 3D Printers in Jaroslavice. The technology of 3D printing at its speed of development has far outweighed the response to its needs. It lacks a new systematically planned building typology corresponding to the requirements of farms. Farms adapt to the spaces. The main aim of the work is to introduce the possibility of turning the situation and adapting the premises to the farms. The thesis follows the urban design of the restructuring of the Jaroslavice landscape elaborated in the previous semester. The project respects established principles at microregion level in the form of emphasis on self-sufficiency, population integrity or the use of current technologies. The land is located on the southern part of Jaroslavice. There are 3 agricultural buildings located on the property, which until 2010, when a photovoltaic power plant was built, operated in conjunction with a neighboring agricultural court. After the power plant was built, the bonds were irreversibly broken. Buildings are in a very poor condition and mutual cooperation no longer works. The existing solution replaces and shows the possibility of using solar energy in a different way. Thus, the construction cartridge works with a hybrid typology where the 3D production area is combined with the maximum solar gains of the photovoltaic panels. Generative methods have been used to design dominantly either for finding a form in terms of achieving maximum solar gains or after verifying the efficiency of the structure. The proposal has several scenarios of possible development. There are four different stages of growth and the linkage of production areas. Printers are able to replicate themselves at such a rate that they can expect rapid growth. The proposal uses controlled growth methods to simulate complex development under the conditions of maximum solar radiation. Visual distraction and overheating are also solved by atypical sunsets on the exterior façade. Thin-film photovoltaic panels are used on the sun, so it is able to produce electricity besides the shield. The energy-efficient shape along with the great advantage of the layout solution, instead of the corridor disposition, is a basic cell on the central plan view. This makes it possible to control and operate more of the machines more efficiently. The production site forwards counts full robot automation.
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Measurement of the properties of cells grown in hydrogel
Belák, Daniel ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
Main subject of this work is to present the possibilities of cultivation of cells in cultivation medium – hydrogel. Topic of the following part is introduction to the problematics of processing of the pictures made by light field microscopy and fluorescence microscopy. It studies the influence of parametres used in the process of stacking the pictures to reach the highest depth of field as possible. Last part is dedicated to measurement of the properties of cell cultures, its statistical processing and analysis.
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Implementation of the Robotic Vacuum Cleaner
Sojka, Stanislav ; Malačka, Ondřej (referee) ; Rozman, Jaroslav (advisor)
This bachelor thesis describes the algorithms and methods of control of robotic vacuum cleaners in an unknown environment. It describes the principles of methods and their limitations. The thesis also describes the implementation of important sections of control algorithms. The work examines problems with localization that have occurred in the control algorithm on creating a real robot. The results of the work are two applications, one for remote control robot according to these algorithms, the second for their demonstration without owning a robot.
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Optical Methods to Evaluate the Contractile Function of Isolated Cardiac Myocytes
Vadkerti, Kristián ; Rychtárik, Milan (referee) ; Čmiel, Vratislav (advisor)
Diploma work is focused on basic characteristics of optical measuring methods commonly used to rate contactions of isolated heart cell and describes basic actions, that are connected with it. A camera record made by a microscope served as basis for optical measuring methods of contractions chosen by us, with using appropriate method elaborating and analysis of pictures used for detection of important part of cell structure. The suggested user application built-up in Matlab environment allows analysis and interpretation of contractional functions in two methodical ways.
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Design and Synthesis of cell nucleus-targeted compounds for use in bioorthogonal reactions
Hrušák, Jáchym ; Vrábel, Milan (advisor) ; Míšek, Jiří (referee)
This bachelor thesis focuses on the design and synthesis of 1,2,4,5-tetrazine and trans- cyclooctene derivatives containing functional moieties which are able to target cellular nucleus and participate in bioorthogonal reactions. Based on literature research, the targeting moieties and the structure of the tetrazine and trans-cyclooctene derivatives were designed with the aim to achieve selective nuclear targeting and to ensure sufficient reactivity of the resulting conjugates under biological conditions. Selected candidates were then synthesized and their properties together with cellular reactivity were investigated in model experiments.
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