|
|
Preparation and testing of SNOM probes
Bobek, Juraj ; Pavera, Michal (referee) ; Spousta, Jiří (advisor)
The area of research that deals with surface modification and preparation of nanostructures is still very unexplored. And only a little contribution to this field is discussed in this bachelor thesis. Its goal is to manufacture and to test a probe made off hollow optical fibre that is used in Scanning Probe Microscopy. Optical fibre parameters in combination with proper and unique techniques allow the breakthrough off very interesting applications. It's worth mentioning the gas injection in the proximity of the surface of a sample (GIS) and thus its modification by use of electrons (FEBID), ions (FIBID) or laser beams.
|
|
|
Drones (UAVs) in the area of emergency services (Integrated Rescue System)
Bobek, Jan ; Baumgartnerová, Alena (referee) ; Rujbrová, Šárka (advisor)
Drony jsou v oblasti integrovaného záchranného systému používány stále častěji, díky jejich lepší dostupnosti a ceně. V policejní oblasti jsou drony využívány k monitorování různých akcí nebo hlídání podezřelých oblastí. Policie drony také využívá k rekonstrukci dopravních nehod. V hasičské oblasti se drony používají hlavně k monitorování požárů a předávání informací pilotovi dronu, ale jsou vyvíjeny i drony určené k hašení požárů. V urgentní medicíně jsou drony využívány k přepravě vybavení a krevních vzorků v místech, kde to pozemními prostředky není možné nebo to je obtížné. Cílem této bakalářské práce je poskytnout přehled dronů využívaných v oblasti integrovaného záchranného systému a popsat technologie související s těmito drony.
|
|
|
Profile-Guided Optimizations for Instruction Caches
Bobek, Jiří ; Zachariášová, Marcela (referee) ; Hruška, Tomáš (advisor)
Instruction cache performance is very important for the overall performance of a computer. The placement of code blocks in memory can significantly affect the cache miss rate. This means that a compiler can improve the performance of a program by placing parts of code at the right addresses in memory. This work discusses several methods for collecting profile information, and describes an algorithm that uses profile information to guide code block placement. Additionally, the algorithm is added into the optimizer of the LLVM compiler, and improvements in cache performance are evaluated.
|
|
|
Design and realisation of solder-ball mounting device
Bobek, Josef ; Szendiuch, Ivan (referee) ; Nicák, Michal (advisor)
This work deals with issues of design and construction of facilities for mounting solder balls. The first part includes a familiarization with the types, manufacture and assembly of solder balls in practice. The following section includes the actual construction work on the equipment, procedures, considerations and finish work on the plant. The last part provides information about solder balls testing, which are mounted on the FRITSCH station, and compares potential errors.
|
|
|
Heating of administrative building
Bobek, Jan ; Počinková, Marcela (referee) ; Topič, Jan (advisor)
The dissertation deals with heating of the administrative building. The building is heated with the floor heating and the panel heating units. The heating source is an air to water heat pump with a bivalent heating from the central heat supplying (CZT). I deal in the project with design, dimension and connection of all these parts.
|
|
|
Preparation and testing of SNOM probes
Bobek, Juraj ; Neuman, Jan (referee) ; Spousta, Jiří (advisor)
Fotonická krystalická vlákna (PCF) představují slibný nástroj pro spojení technik známých z mikroskopie rastrovací sondou, elektronové mikroskopie a systémů pro vstřikování plynu. Přivedení světla a přenos pracovního plynu současně do blízkosti vzorku umístěného uvnitř elektronového mikroskopu přináší nové možnosti experimentů. PCF by mohly být použity nejen k charakterizaci nebo modifikaci struktur na mikroskopické úrovni, ale také k jejich výrobě. Tato práce se zabývá výzkumem literárních zdrojů s tématikou fotonických krystalů s důrazem na PCF. Leptání PCF pomocí kyseliny fluorovodíkové bez poškození jejich vnitřní struktury je experimentálně studováno s velkou precizností. Optické vlastnosti závislé na geometrii PCF jsou testovány pro různé modifikace PCF. Dále se práce zabývá spojením PCF s mikroskopií atomárních sil a následnou integrací do elektronového mikroskopu.
|
|
|
Analysis of secondary metabolites from Streptomyces by Raman spectroscopy in vivo
Mikulová, Adéla ; Petříčková, K. ; Bobek, J. ; Chroňáková, Alica ; Samek, Ota ; Pilát, Zdeněk
The work deals with the study of Raman spectra of secondary metabolites of bacteria of the genus Streptomyces, especially polyene antifungal agents. Purified isolates and commercial standards of these compounds were used to obtain reference spectra from which spectral markers characterizing specific details of the chemical structure were derived. Verification of the observed markers was performed using in silico Raman spectra predictions. Subsequently, these findings were used for the spectroscopic characterization of the composition of secondary metabolites in vivo, directly in bacteria growing on a solid medium. The developed methodologies will help to optimize the conditions for the biotechnological production of antibiotics.
|
|
|
6S RNA as a tool for increased production of clinically relevant antibiotics
Kostílek, Štěpán ; Bobek, Jan (advisor) ; Ulrych, Aleš (referee)
Streptomyces belong to the group of soil microorganisms, and they are significant producers of biologically active substances used in a wide range of industries, whether it is medicine, agriculture or biotechnology. It is for this reason that the research of these microorganisms is of interest to a number of research institutions. One of the most important groups of these biologically active substances are antibiotics. A significant part of antibiotics produced in nature is produced precisely by streptomyces, and therefore it is in our interest to understand the mechanisms that influence this production. A wide variety of different regulatory mechanisms controlling antibiotic production have been found so far in streptomyces. One of these regulatory mechanisms is the utilization of scr3559 RNA. Scr3559 RNA is a small, non-coding RNA molecule that regulates antibiotic production at the level of DNA transcription. These small RNA molecules mimic the structure of the target promoter sequence and are able to interact with RNA polymerase. The presence of scr3559 RNA has not been confirmed in streptomyces as of yet, but new scr3559 RNA molecules are still being sought. To find new molecules, in silico methods are used, where the similarity of the scr3559 RNA sequence of S. coelicolor with homologous...
|
|
| |
|
|
The effect of 6S-like RNAs on physiological differentiation of Streptomyces coelicolor
Burýšková, Barbora ; Bobek, Jan (advisor) ; Branny, Pavel (referee)
The variety of bacteria and their genomes sometimes causes conservation of homologue molecules to be displayed not in sequence but in secondary and tertiary structures. In the case of the regulatory 6S RNA, sequence homologues have been found in over 100 bacterial species so far. However, none were found in the genus Streptomyces. The unique genome of these soil- dwelling bacteria, known for their capacity to produce antibiotics, has a high G/C content and diverges substantially from distantly related bacteria. Yet in the non-coding 6S RNA it is the secondary structure that is crucial for its function. The 6S RNAs trap sigma factors by mimicking target promoter sequences in order to help with switching sets of expressed genes during developmental transitions. 6S-like RNA genes in Streptomyces coelicolor have been computationally predicted by comparison of in silico modelled secondary structures of known 6S RNAs. The aim of this thesis was the verification of these 6S-like RNA predictions. The experimental approach was based on RNA co-immunoprecipitation (RNA CoIP), as well as RT- PCR from RNA samples. The outcomes of this project are the detection of six novel ncRNA transcripts with possible 6S-like RNA functions, which also served as the wet-lab verification of the in silico prediction technique...
|
guest ::
RSS feed.