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Physical activity and sports history of children 11 to 19 years old
Babický, Lukáš ; Nováková, Tereza (advisor) ; Žáková, Lenka (referee)
Title: Physical activity and sports history of children 11 to 19 years old Objectives: To analyse the level of leisure physical activity and its specifics on a sample of the adolescent population. To analyse the activity development from the historical perspective of the proband from the initial experience with organized physical activity. The results of the survey are further compared with the conclusions of previous studies on the issue. Methods: A total of 563 respondents (235 boys and 328 girls) from Prague's Gymnasiums (secondary grammar schools) took part in the research. Data were obtained using a questionnaire survey (CAWI) based on preliminary research and evaluated using MS Excel, GraphPad Prism and mathematical software R. Results: The preference of single sport (63.8 %) was found in the examined population with a predominance of the frequency of 2 to 4 trainings per week (61.2 %). The vast majority (93.6 %) stated that they were participated fundamental movement training at a younger school age. More than half (61 %) experienced pain or injury within physical activity, especially in the lower limbs (61.9 %). Less than half of respondents (46.3 %) have already visited a physiotherapist. The main factor in the cessation of physical activity is psychosocial reasons (76.9 %). Increasing age...
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Clinical evaluation of ROM of shoulder joint in cadet and junior category players of so-called overhead sports
Benda, Petr ; Nováková, Tereza (advisor) ; Žáková, Lenka (referee)
The title of the master thesis: Clinical evaluation of ROM of shoulder joint in cadet and junior category players of so-called overhead sports Abstract in English language: The goal of this thesis is to elucidate whether the "overhead" athletes like handballers and volleyballers suffer from extension changes and local hypermobility in their shoulder joints. These changes are going to be described by trigonometric measurements and hypermobility tests by Sachse. It is going to be determined which "overhead" athletes are affected the most. Their sex is going to be taken into consideration. The theoretical part of the thesis describes selected "overhead" sports and their strain on a human body from a kinesiologic point of view. Especially the impact of offensive blows in volleyball and throws in handball on shoulder joints are going to be examined. The thesis is going to examine a shoulder girdle from an anatomy, kinesiology, biomechanics, and physiology point of view. A chapter focused on hypermobility and it is classification is included too. 95 people without a prior injury of a shoulder joint in the age from 16 to 19 years old including 73 "overhead" athletes were tested. There were 30 men, 40 women, 34 handballers, and 39 volleyballers. In the control group, there were 22 people, of which 11 men and 11...
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The preparation and characterisation of analogues of insulin and IGF-2 selective for both isoform of insulin receptor and IGF-1 receptor
Mlčochová, Květoslava ; Žáková, Lenka (advisor) ; Obšil, Tomáš (referee) ; Šulc, Miroslav (referee)
Insulin and insulin-like growth factor 1 (IGF-1) and 2 (IGF-2) are related protein hormones with different but overlapping biological functions. All the hormones interact with a receptor within the insulin-IGF system (insulin receptor A and B, IGF-1 receptor), however with different affinity. The different interaction with individual receptors is just one of the main tools for regulation of the system that is essential for the proper functioning of the organism. Although the residues directly interacting with receptors are mainly located in A and B domains, the C and D domains probably play a role in receptor specificity. Here, we firstly focused on the impact of D domains of IGF-1 and 2 (D1 and D2 domains) and C domain of IGF- 2 (C2 domain). To probe the impact of C and D domains, we prepared insulin analogues containing a part of or an entire domain following a pattern seen in IGFs. The receptor-binding affinities of these analogues and their receptor autophosphorylation potentials were characterised. Our results revealed that the initial part of D1 domain has a detrimental effect on IR affinity that is only slightly enhanced by the rest of the D1 domain. D2 domain has rather neutral effect on IR affinity. We further showed that the addition of amino acids derived from the C2 domain to the...
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Evaluation of the strength of selected muscle groups and the range of motion in sport aerobics athletes
Sílová, Markéta ; Žáková, Lenka (advisor) ; Vomáčková, Helena (referee)
Title: Evaluation of the strength of selected muscle groups and the range of motion in sport aerobics athletes Objective: Sport aerobics belongs to group of sport gymnastics, which brings increased demands especially on joint mobility, muscle strength and coordination of movement. Athletes are selected according to these prerequisites the development of which is still supported by training. The thesis follow up the physical measurement of these attributes. The Aim: Evaluation of joint mobility and muscle strength of 12-16 year old sport aerobic athletes and their correlations. Furthermore, the comparison together with the level of the performance group. Method: Measurement of joint mobility using goniometry, hypermobility evaluation using Beighton score, measurement of the muscle strength using HUMAC isokinetic dynamometer. Comparison of measured results with performance group level. Results: During measurement of the joint range using the method goniometry was found, that in flexion of the hip joint had 100 % of the athletes increased joint motion. In hip extension, the joint range wasn't increased at all. Beighton score showed, that 90 % of athletes were constitutionally hypermobile. The relationship between joint range and the values of the muscle strength - peak torque has not been proven. Key...
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Preparation of preptin, a peptide secreted from pancreatic islet beta cells, and its effect on insulin secretion
Zrubecká, Denisa ; Žáková, Lenka (advisor) ; Dračínská, Helena (referee)
in English Pancreas is an endocrine tissue which affects many physiological processes in organism. It has been known for a long lime that the pancreatic beta cells secret insulin. However, there are more hormones and substances secreted from the beta cells, mainly peptides such as insulin-like growth factors IGF, shorter peptides like amylin and many more. Some of them have not been even identified. Among these pancreatic products Ch. Buchanan's group from Auckland University, New Zeland identified 34 amino acids long peptide called preptin in 2001. Its amino acid sequence is identical with part of the preform of insulin-like growth factor 2 chain. It has been discovered that preptin has effect on osteoblast proliferation and insulin secretion. Nowadays preptin is being studied like a potential osteoporosis cure thanks to its increasing effect on osteoblasts proliferation. This thesis describes preparation and characterization of human and rat preptin and their two fragments (which contain amino acids 1-16 and 17-34) using solid-phase synthesis. The aim of the thesis is to test effects of rat preptin and its two fragments on insulin secretion in pancreatic beta cell line INS-1E. Part of the thesis is focused on binding assays of human preptin and its two fragments to IGF-1 receptor and also to...
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Use of Art Therapy in Social Work
ŽÁKOVÁ, Lenka
The main topic of this bachelor´s work is use of art therapy in social work. In the first under chapter of theoretic part I define the art therapy, it´s history, it´s forms which I split up in the individual part and the group part and I also define the education of the art therapist. Further I mention target groups such as children, adults and seniors. I also describe the different stances in art therapy. There are some methods like imagination, animation, concentration, shakeout, transformation and reconstruction. At the end of the first under chapter I have described technics of art therapy. Second under chapter contains the concept of social work, the role of the social worker and his education and social services. The practical part of this work contains qualitative research with use of technic of half standardized interview and with questioning method. The half standardized interviews were with five social workers and two art therapists who work in Brno in social services. The main goal of this bachelor´s work was to map possibilities of using the art therapy in social services. Particular goals were to find out, if the art therapy helps the users in social services, in what exactly does art therapy help and in which situations it´s most convenient to use the art therapy. Research questions are concentrated on application of art therapy in social services, on methods and technics of art therapy, theirs use in the context of social work and in this work is also shown the importance of working cooperation between social worker and art therapist. In my opinion I have full filed the results and I have found that the art therapy helps the clients with solving demanding situations, under stress and also just in a bad mood. Best benefits are development of communication, self-expression and connecting contacts. In my opinion the results of my survey could be used by social workers and also the art therapist. The results can be used by facilities which will get basic information about other types of facilities and they can improve their own deficiency. I also guess that this work can be interesting for the students who study social work or for the art therapists who want to concentrate themselves for social area.
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Determination of binding constants of human insulin complexes with serotonin, dopamine, arginine, and phenol by pressure assisted partial filling affinity capillary electrophoresis
Šolínová, Veronika ; Žáková, Lenka ; Jiráček, Jiří ; Kašička, Václav
A new method, pressure assisted partial filling affinity capillary electrophoresis (PF-ACE), has been developed to study noncovalent interactions of the hexamer of human insulin (HI) with cationic ligands, such as phenolic neurotransmitters serotonin and dopamine, and amino acid arginine, or with anionic ligand phenol, in alkaline aqueous solutions. The apparent binding constants, Kb, of the HI-ligand complexes were determined from the dependence of the effective migration time changes of the above ligands on the variable zone lengths of HI dissolved in the background electrolyte and hydrodynamically introduced into the bare fused silica capillary close to the UV detector. The HI interactions with the above ligands were found to be moderately strong, with Kb values in the range 385-1314 L/mol.
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The role of amino acids from the C-terminus of the insulin B chain in the selectivity towards insulin receptor isoforms.
Polák, Marek ; Žáková, Lenka (advisor) ; Obšilová, Veronika (referee)
The main function of insulin as a hormone is to control glucose homeostasis in the blood. This control takes place at different levels in different parts of the body. The complexity of the regulation of blood glucose levels is manifested through the insulin receptor (IR) and its two isoforms, IR-A and IR-B. IR-B is responsible for metabolic effects and the distribution in adipocytes, muscles and hepatic cells, whereas IR-A has, above all, mitogenic effects with lymphocytes, spleen, brain and cancer cells. Today's treatment of diabetes patients is focused on the use of insulin analogues, insulin replacemensts with a different IR-A and IR-B binding affinity. Today's patients use two different types of analogues, called fast-acting and basal, with a focus on the combination of these two types throughout the day. In 2011, prof. Belfiore from the Universtiry of Catanzzaro published a scientific article about the close relation between diabetes and some cancer types. This article triggered extensive debates about the impact of insulin or insulin analogues on the mitogenic isoform IR-A of the insulin receptor, and IR-A isoform on the insulin-like growth factor type 1. In the same year, two scientific groups from a Danish company Novo Nordisk, published the first hints at receptor isoform-selective insulin...
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Study of interactions between low-molecular mass compounds and the DNA-binding domain of forkhead transcription factor FOXO3
Kohoutová, Klára ; Obšil, Tomáš (advisor) ; Žáková, Lenka (referee)
This bachelor thesis is part of a project focused on studying low-molecular mass compounds able to inhibit the interaction between DNA-binding domain of human forkhead transcription factor FOXO3 and the target DNA. FOXO3 is one of four members of FOXO class transcription factors which belong to forkhead family of transcription factors. Forkhead transcription factors are evolutionary conserved proteins playing important roles in numerous cellular processes. These include cell-cycle regulation, oxidative stress response, control of cellular metabolism and apoptosis. FOXO3 plays an important role in cancer cells where it acts not only as a tumor suppressor but also can enhance their resistance to chemotherapy. Considering its biological functions, the study of small-molecule inhibitors of FOXO3 transcription factor is of particular importance. This bachelor thesis was focused on compound S9 oxalate as a potential inhibitor of FOXO3-DNA interaction. Main goals of this thesis were: (I) preparation of both unlabeled and 15 N labeled DNA- binding domain of FOXO3 transcription factor, (II) characterization of interactions between FOXO3 DBD and compound S9 oxalate using NMR and electrophoretic mobility shift analysis (EMSA), and (III) prediction of binding conformation and interactions between FOXO3 DBD and...
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The effect of vandetanib, lenvatinib and ellipticine on the expression of rat cytochromes P450 1A and 3A
Jelínková, Sandra ; Dračínská, Helena (advisor) ; Žáková, Lenka (referee)
In recent years, the inhibiition of tyrosine kinases,which may incorrectly regulate some singaling pathway has been used to treat cancer as so-called biological therapy. An example of such inhibitors are vandetanib and lenvatinib. These two substances are used to treat thyroid gland tumors because they affect vascular growth factor receptor or endothelial growth factor receptor that can regulate tumor growth and metastasis. Ellipticine, which has anti-tumor effects on lots of tumor disease, has been investigated in this study together with vandetanib and lenvatinib. In this diploma thesis, the effect of mentioned tyrosine kinase inhibitors, ellipticine and their combinations on gene and protein expression of CYP1A1, 1A2, 3A1 and 3A2 in rat liver in vivo was determined. Protein expression was studied using Western blot method with imunodetection. Gene expression was assessed by quantitative PCR. Moreover, the effect of tested substances and their combinations on CYP1A activity (measured as 7-ethoxyresorufin O-deethylation), CYP1A2 activity (measured as 7-methoxyresorufin O-demethylation), CYP1A1 activity (measured as Sudan I oxidation), CYP3A specific activity (measured as testosteron 6β-hydroxylation) and ellipticine, vandetanib, lenvatinib metabolism was determined. It has been confirmed that...
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