Original title: Determination of binding constants of human insulin complexes with serotonin, dopamine, arginine, and phenol by pressure assisted partial filling affinity capillary electrophoresis
Authors: Šolínová, Veronika ; Žáková, Lenka ; Jiráček, Jiří ; Kašička, Václav
Document type: Papers
Conference/Event: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Year: 2018
Language: eng
Abstract: A new method, pressure assisted partial filling affinity capillary electrophoresis (PF-ACE), has been developed to study noncovalent interactions of the hexamer of human insulin (HI) with cationic ligands, such as phenolic neurotransmitters serotonin and dopamine, and amino acid arginine, or with anionic ligand phenol, in alkaline aqueous solutions. The apparent binding constants, Kb, of the HI-ligand complexes were determined from the dependence of the effective migration time changes of the above ligands on the variable zone lengths of HI dissolved in the background electrolyte and hydrodynamically introduced into the bare fused silica capillary close to the UV detector. The HI interactions with the above ligands were found to be moderately strong, with Kb values in the range 385-1314 L/mol.
Keywords: affinity capillary electrophoresis; human insulin; ligand
Project no.: GA17-10832S (CEP), GA18-02597S (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Original record: http://hdl.handle.net/11104/0291135

Permalink: http://www.nusl.cz/ntk/nusl-390682


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
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