|
|
Biology of non-commensal populations of house mouse (Mus musculus sensus lato) from the Near East
Slábová, Markéta ; Frynta, Daniel (advisor) ; Macholán, Miloš (referee) ; Sedláček, František (referee)
Proceedingseditor: SlábováM. & Sýkorová,Z. (eds.) (2006): Proceedings of the InternationalPh.D. students'coďerence. Universityof SouthBohemia in ČeskéBudějovice,Facultyof Agriculture,ČeskéBudějovice,Czech Republic,2006. Sýkorová Z. & Slábová M. (eds.) (2007): Proceeding of the IntemationalPh.D. students'conference.Universityof SouthBohemia in ČeskéBudějovice,Facultyof Agriculture,ČeskéBudějovice,Czech Republic,2007. Grants: 2000-2005participantontheprojectGAAV IAA6141102 Licences: Licencefor experimentaltreatmentwithanimals(numberCZU 540102) allowedby CzechCentralCommissionfor Animď Welfare. Summary House mouse(Mus musculussensulato) is one of the most studied mammals, but the attentionis paid mostly to laboratory (strictly speakingdomesticated)'or coÍnmensalpopulations.But thereare also non-coÍnmensalpopulations,living in totally differentconditionsand undertotallydifferentselectivepressures,sotheknowledgeconceming conrmensalÁaboratorypopulations can not be generalized. The evolutionof commensalismdefinitelycausedlarge changesin house mouselife history,behaviour,morphologyandsocialorganization.The aim of this studywasto, at leastpartially,fill in thi. gupin knowledge abouthousemouse,reallyinterestingandwell adaptablespecies. 4 Thethesisfocuseson thenon-commensalpopulationsaf Mus musculus domesticusfrom the...
|
|
| |
|
|
Genus Apodemus (Mammalia: Rodentia) in the Quaternary fossil record of Central Europe.
Knitlová, Markéta ; Horáček, Ivan (advisor) ; Macholán, Miloš (referee) ; Čermák, Stanislav (referee)
Wood mice of the genus Apodemus rank among the most common extant mammals throughout Europe. A history of its species and their distributional ranges is to be looked upon as a serious topic relevant also for general concepts on current setting of European biotic diversity. Nevertheless, despite several phylogeographic studies on that subject and a rich fossil record of the genus, many details of that issue remains only very poorly comprehended. This state is essentially caused by enormous complications with phenotype discrimination of individual species, their extensive variation and broad between-species overlaps in all phenotype traits. The present project was intended to establish the techniques enabling reliable species identification applicable to fragmentary fossil materials and to use them for retrieving information on range dynamics and phylogenetic history of individual species during the Quaternary past. It begun with detailed morphometric analyses of extant populations, using the characters available in fossils, and with establishing a concept of alternative parataxonomic classification covering the complete phenotype span of the genus. The respective techniques were applied onto an extensive set of well-dated records from the Late Pleistocene-Holocene sedimentary series from diverse...
|
|
|
The impact of geography and subsistence on distribution of NRY haplogroups in Europe and Africa
Nováčková, Jana ; Černý, Viktor (advisor) ; Munclinger, Pavel (referee) ; Macholán, Miloš (referee)
Y chromosome is due to its special characteristics the ideal tool of archaeogenetic studies. Its diversity is influenced by several factors and I analysed two of them (geographical location and subsistence). I generated SNPs and STRs data from several loci of samples from Slovakia (156 samples, 5 regions) and sub-Saharan Africa, where I analysed samples of sedentary farmers (481 samples, 18 regions) and nomadic pastorals (405 samples, 16 regions). Slovakia is situated at the meeting point of two migration ways. First of them was spread from the east to the west and is associated with enlargement of haplogroup R1a in Europe. The second came from the Iberian Peninsula eastward and is associated with enlargement of haplogroup R1b. Results of MDS graphs replicate the geographical map of Europe. Slovakia is situated in the middle of Russian, Balkanian and Iberian samples. Correlation between genetics and geographic distances is indicated by hierarchical AMOVA analysis and Mantel tests. Populations in sub-Saharan Africa differ from each other by the subsistence pattern. Different life style influence the diversity of the Y chromosome. Nomadic pastoralists and sedentary farmers share different haplogroups, for example, while haplogroup R1b was detected only in nomadic pastoral groups, sedentary farmers...
|
|
|
Population of African Sahel according to phylogeography of Eurasian haplogroups
Kulichová, Iva ; Černý, Viktor (advisor) ; Macholán, Miloš (referee)
The thesis is focused on two mtDNA haplogroups of Eurasian origin that occur mostly among the Fulani, herders of the African Sahel. On the initial analysis 544 DNA samples from unrelated Fulani individuals were used. These samples were classified to haplogroups according to hypervariable segment I (HVS-I) of mtDNA and subsequently, specific Eurasian haplotypes were chosen for the whole mtDNA genome sequencing. Obtained mito-genomes were assigned to phylogenetic trees and dated. It turned out that they belonged to haplogroups U5b1b1b and H1ca1a with the probable origin in the Iberian Peninsula, presumably in Franco-Cantabrian refugium where their ancestors originated in the period between the Late Glacial and the first half of the Holocene. Afterwards they migrated through the Strait of Gibraltar to North Africa and the Sahara, where the proto-Fulani pastoral population was being formed. It may also be assumed that a part of this population came to Africa from the Near East, along with cattle. In the second half of the Holocene, this pastoral population migrated from the drying Sahara to the Sahel. Probably due to small number of female migrants the females from the local populations were integrated, which explains the major representation of West African mtDNA haplogroups in contemporary Fulani....
|
|
|
Behavioural patterns exhibited by three populations of house mouse ( Mus musculus lato) in five-tests battery: the effects of subspecies and commensal way of life
Voráčková, Petra ; Frynta, Daniel (advisor) ; Macholán, Miloš (referee)
The term "personality" nowadays occurs more often not only in psychological studies of humans but also in animal studies. Studying of personality help us to define the behavioural characteristics which can vary within the age, sexes, species or enviroments. Behavioral experiments are used to detect these behavioral patterns and they can divide the animals into the different groups. The subject of our research became three populations of house mouse (Mus musculus sensu lato) which we tested in a series of experiments involving free exploration, forced exploration, hole- board test, test of vertical activity and Elevated plus-maze. These experiments should reveal wheter the mice differ in their behaviour through the context of sex, comensalism or subspecies. We found (with in excepcion of one test) that intrapopulation variability differences are very small but interpopulation differences purely increase in the cas of comensalism and effects of subspecies. Keywords: Mus musculus, comensalism, open fieldtest, Elevated plus-maze, Principal Component Analysis (PCA)
|
|
|
Shape variability of proximal and distal human tibia.
Brzobohatá, Hana ; Velemínská, Jana (advisor) ; Beňuš, Radoslav (referee) ; Macholán, Miloš (referee)
The aim of this study was to detect and describe the trends in shape variability of proximal and distal human tibia related to sex, age, social status and time period. The variability of its shape and form was analysed first in an early medieval sample, then in two modern populations, an early 20th century sample and the contemporary Czech population. Finally, data from the whole studied time span were analysed. The initial raw data were acquired by optical scanning and computed tomography (CT) scanning of the lower limbs; the three-dimensional bone surfaces of the contemporary tibias were segmented from the clinical CT scan sequences. Geometric morphometrics and multivariate statistical methods were applied to study the variations in shape and form quantitatively. Proximal and distal tibias were evaluated independently, and the potential influence of sex, age at death and time period were investigated. In the early medieval population, we also explored the relationship between tibial morphology and presumed social status derived from the respective grave location within the settlement. Because traditional morphometric analysis revealed statistically significant sex differences in the parameters of both tibial extremities (e.g., Steyn and Işcan, 1997; Šlaus et al., 2013), we thus assumed the...
|
|
|
Population structure and migrations in the African Sahel as approached from the variability of control region of mtDNA
Mašek, Jan ; Černý, Viktor (advisor) ; Macholán, Miloš (referee)
Although West Africa is region rich in its cultural and lingvistical diversity, patterns and distributions of genetic diversity are still not fully understood. Most of the studies dealing with the population genetics of mitochondrial DNA comes from the variability of its hypervariable segment (HVS-1) only. In this diploma work I have focused on whole control region aiming for determination of the contribution of HVS-2 segment to phylogenetical classification and explanation of genetic relationship in African Sahel. To investigate these two goals 1075 sequences from 24 populations have been analyzed (104 sequences comes from this study, the rest was publicated previously by other authors). The results indicated that HVS-2 has a specifying meaning in the phylogenetical classification in case of complete reference database is available. On the population level African Sahel seems to be quite homogeneous and its populations are mostly differentiated from Saharan groups. It seems that geographic factors have bigger impact on distribution of genetic diversity then the lingvistic ones. Close genetic relationships of West African populations and their difference with Saharan populations suggests a higher importance for migratory corridor along Sahel belt.
|
|
|
Fylogeneze vybraných druhů letounů Afriky na základě cytogenetického a molekulárního přístupu
Koubínová, Darina ; Zima, Jan (advisor) ; Macholán, Miloš (referee) ; Martínková, Natália (referee)
Phylogenetic relationships of a sample comprising 248 bats belonging to 19 species and four families (Hipposideridae, Rhinolophidae, Molossidae and Vespertilionidae) from Senegal (Western Africa) were investigated with the use of multi-locus sequence data and non- differentially stained chromosomes. The karyotypes of Hipposideros ruber, H. tephrus, H. jonesi and H. cyclops were described for the first time. The standard Hipposideros formula was recorded in H. tephrus, H. jonesi and H. ruber (2n = 32, FNa = 60, FN = 64). The karyotypes of H. cyclops (2n = 36, FN = 66) and H. gigas (2n = 52, FN = 64) substantially diverged from this typical chromosomal complement. Rhinolophus landeri and R. fumigatus shared the same diploid number (2n = 58), but differed in the chromosome morphology (R. fumigatus - FNa = 60, FN = 64; R. landeri - FNa = 64, FN = 68). Rhinolophus landeri was found karyotypically distinct to other African populations, thus signalling a possible presence of cryptic forms within this species. The karyotypes of Chaerephon pumilus and Mops condylurus had a 2n = 48, FN = 54 and were similar to other previously studied species of this chromosomally conservative family. Chromosomal, Bayesian, maximum likelihood and genetic distance analyses revealed an indication for the existence of cryptic...
|
|
|
Genetics and Genomics of Hybrid Sterility
Bhattacharyya, Tanmoy ; Forejt, Jiří (advisor) ; Pravenec, Michal (referee) ; Macholán, Miloš (referee)
Charles University in Prague Faculty of Science Ph.D. study program: Molecular and Cellular Biology, Genetics and Virology Abstract Genetics and genomics of hybrid sterility Mgr. Tanmoy Bhattacharyya Supervisor: Prof. MUDr. Jiří Forejt, DrSc. Praha 2013 Abstract Male-limited hybrid sterility restricts gene flow between the related species, an important pre- requisite of speciation. The F1 hybrid males of PWD/Ph female (Mus m. musculus subspecies) and C57BL/6J or B6 male (Mus m. domesticus) are azoospermic and sterile (PB6F1), while the hybrids from the reciprocal (B6PF1) cross are semi fertile. A disproportionately large effect of the X chromosome (Chr) on hybrid male sterility is a widespread phenomenon accompanying the origin of new species. In the present study, we mapped two phenotypically distinct hybrid sterility loci Hstx1 and Hstx2 to a common 4.7 Mb region on Chr. X. Analysis of meiotic prophase I of PB6F1 sterile males revealed meiotic block at mid-late pachynema and the TUNEL assay showed apoptosis of arrested spermatocytes. In sterile males over 95% of pachytene spermatocytes showed one or more unsynapsed autosomes visualized by anti SYCP1, HORMAD2 and SYCP3 antibodies. The phosphorylated form of H2AFX histone, normally restricted only to XY chromosome containing sex body decorated unsynapsed...
|
guest ::
RSS feed.