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Construction and evaluation of a novel protein mechanosensor
Kolomazníková, Veronika ; Rösel, Daniel (advisor) ; Novotný, Ivan (referee)
The protein p130Cas (human ortholog BCAR1) is a major substrate for phosphorylation by the Src family kinase and plays a central role in oncogenic transformation. Increased level of BCAR1 correlates with primary tumour growth and cancer progression. Localized to focal adhesion, p130Cas serves as a mechanosensor and mediates key interactions with the extracellular environment. The structure of p130Cas is crucial for its function, mainly the anchoring domains SH3 and CCH, together with the substrate domain which is extended when under tension. This Master's thesis presents a newly developer FRET mechanosensor based on the structure of p130Cas. The sensor utilizes the anchoring domains of p130Cas for proper localization to focal adhesions, where it can detect tension in living cells. Key words: p130CAS, FRET, focal adhesions, mechanosensing
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Marketing Plan for company ISSO s.r.o.
Novotný, Ivan ; Kaňovská, Lucie (referee) ; Šimberová, Iveta (advisor)
The main goal of this bachelor´s thesis is to prepare a draft marketing plan for ISSO, s.r.o.. In the theoretical part, knowledge about marketing, marketing communication is processed. The second analytical part includes the processing of the current situation of the company on the market. In the last part, I propose a comprehensive marketing plan that takes care of the chosen goals.
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Marketing Plan for company ISSO s.r.o.
Novotný, Ivan ; Kaňovská, Lucie (referee) ; Šimberová, Iveta (advisor)
The main goal of this bachelor´s thesis is to prepare a draft marketing plan for ISSO, s.r.o.. In the theoretical part, knowledge about marketing, marketing communication is processed. The second analytical part includes the processing of the current situation of the company on the market. In the last part, I propose a comprehensive marketing plan that takes care of the chosen goals.
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Grafting VTMOS onto PHB
Novotný, Igor ; Petruš, Josef (referee) ; Kučera, František (advisor)
Diploma thesis deals with the grafting of vinyltrimethoxysilane (VTMOS) onto poly(3–hydroxybutyrate)PHB. Subsequent characterization of the amount of grafted VTMOS and changes in the thermal properties associated with the rate of crystallization. The theoretical part deals with mechanism and the influences of grafting. In the experimental part VTMOS was grafted onto PHB without subjecting VTMOS to hydrolysis and subsequent crosslinking. By differential scanning calorimetry (DSC) and Avrami equation, the effect of grafted silane group on pure was studied. The MVR was used to compare the rheological properties of initial PHB, grafted PHB and crosslinked PHB by siloxane linkages.
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Identification of risks in the process of marketing strategy formulation and realisation and their solving
Novotný, Igor ; Válka, Vít (referee) ; Chalupský, Vladimír (advisor)
The aim of this thesis is to identify the risk, emerging from use of the marketing attitude in ALL SPORTS ltd., qulify its potential impact to the operation of the company and suggest the proceeding to minimize the negative impacts. First part includes theoritical resources for exploring of the aim, there is explained basic terminology and described technique, styles and analyses that are necessary for identifying the risks, identification of the risk situation and ratio of the probability that risk situation will occure. Second part of the thesis is practical and theoretical informations mentioned in first part are applied for identification and valuation of the risks. On the basis of performed analyses I identified risks and ratio of the probability for occuring. In the last part of the thesis I described proceeding for elimination of these risks including quantification of the cost.
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Formování sestřihových snRNP v buněčném jádře
Novotný, Ivan ; Staněk, David (advisor) ; Cmarko, Dušan (referee) ; Forstová, Jitka (referee)
1 ABSTRACT There are many structures, suborganelles and bodies in the eukaryotic cell nucleus. These domains provide the nucleus with many specific functions. Nucleolus is specialized compartment serves to ribosomes assembly, nuclear speckles or Splicing Factors Compartment play an important role in RNA processing and best studied of them, Cajal bodies (CBs), are involved in snRNP maturation. However, non-membrane substructures are not unique for cell nucleus; processing bodies (P bodies) found in the cytoplasm are proposed to be important places in mRNA degradation pathway. This work is a compilation of four projects focused on non-membrane cellular bodies; namely, nuclear CBs and cytoplasmic P bodies. Both CBs and P bodies are dynamic structures that continuously exchange their components with surrounding environment. In addition to a widely accepted role of CBs in snRNP biogenesis, we show that the CB serves as a place where snRNPs are regenerated after each round of splicing. Thus, CBs are important nuclear compartment involved in snRNP recycling. To further characterize tri-snRNP assembly in CBs we applied kinetic experiments combined with mathematical modeling and created a kinetic model of tri- snRNP formation in the CB that determined kinetic parameters of tri-snRNP formation. Moreover, our kinetic...
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The role of cyclophilins in splicing
Cit, Zdeněk ; Půta, František (advisor) ; Novotný, Ivan (referee)
Splicing is a process essential for the proper functioning of eukaryotic cells. It is a complicated and highly dynamic process, participated by large numbers of proteins which perform diverse functions, either directly within the splicing complex, or sometimes outside of it. Among the proteins, which play an important role in splicing, are cyclophilins. Cyclophilins probably cause conformational changes in the other components of splicing complex. They can also maintain them in the desire conformation thereby they contribute to the dynamics of the spliceosome. This work provides an overview of cyclophilins, which were confirmed to participate on pre-mRNA splicing, and summarizes suggestions of possible functions that these proteins may perform.
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Development of the II. phase of the Master Plan of Water Management in the Czech Republic
Trnka, Miroslav ; Dumbrovský, M. ; Růžek, P. ; Žalud, Zdeněk ; Drbal, J. ; Hlavinka, Petr ; Novotný, I. ; Vopravil, J. ; Chuchma, F. ; Vizina, P.
This report is processing research results done within the contractual cooperation of the Institute of Global Change Research of the Czech Academy of Sciences (as a research provider) and Mendel University in Brno (demander), which was prepared in collaboration with other research partners. The report processes II. phase of the Master Plan of Water Management in the Czech Republic with regard to the ongoing climate change.\n\n
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Balancing Assessment of Available Water Resources and Moisture Needs within Defined "Irrigation Districts”
Trnka, Miroslav ; Hlavinka, Petr ; Balek, Jan ; Semerádová, Daniela ; Dubrovský, Martin ; Štěpánek, Petr ; Vizina, A. ; Hanel, M. ; Žalud, Z. ; Lukas, V. ; Dumbrovský, M. ; Růžek, P. ; Daňhelka, J. ; Chuchma, F. ; Novák, P. ; Novotný, I. ; Pavlík, F.
This report is processing research results done within the contractual cooperation of the Institute of Global Change Research (as a research provider) and Státní pozemkový úřad - the Czech National Land Authority (demander), which was prepared in collaboration with other research partners. The report deals with balancing assessment of available water resources and moisture needs within defined "irrigation districts” of the Czech Republic with regard to the ongoing climate change
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Representative of drama education in South Bohemia - Jirina Lhotská
NOVOTNÝ, Ivan
The thesis deals with the work of Mgr. Jiřina Lhotská and her contribution to the drama in education in the South Bohemia County. The process of drama in education teaching and its basic elements are described in the theoretical part of the thesis. In the second part focuses on the character of Jiřina Lhotská, her career and contribution to the drama in education teaching. Furthermore, the practical part also contains interviews with individuals who profoundly influenced Jiřina Lhotská the pedagogue. The interviews with her colleagues and students with whom she has cooperated during various stages of her career (up until now) is also included therein. The lists of Jiřina Lhotská's creative activities, selected scripts of certain acts, as well as some photographs form part of multiple appendices.
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