Formování sestřihových snRNP v buněčném jádře

Novotný, Ivan

1 ABSTRACT There are many structures, suborganelles and bodies in the eukaryotic cell nucleus. These domains provide the nucleus with many specific functions. Nucleolus is specialized compartment serves to ribosomes assembly, nuclear speckles or Splicing Factors Compartment play an important role in RNA processing and best studied of them, Cajal bodies (CBs), are involved in snRNP maturation. However, non-membrane substructures are not unique for cell nucleus; processing bodies (P bodies) found in the cytoplasm are proposed to be important places in mRNA degradation pathway. This work is a compilation of four projects focused on non-membrane cellular bodies; namely, nuclear CBs and cytoplasmic P bodies. Both CBs and P bodies are dynamic structures that continuously exchange their components with surrounding environment. In addition to a widely accepted role of CBs in snRNP biogenesis, we show that the CB serves as a place where snRNPs are regenerated after each round of splicing. Thus, CBs are important nuclear compartment involved in snRNP recycling. To further characterize tri-snRNP assembly in CBs we applied kinetic experiments combined with mathematical modeling and created a kinetic model of tri- snRNP formation in the CB that determined kinetic parameters of tri-snRNP formation. Moreover, our kinetic...

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