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Mitochondrial respiration at cold acclimated rats. Comparison of tissues.
Flégrová, Eliška ; Žurmanová, Jitka (advisor) ; Nováková, Olga (referee)
Acclimation to cold or hardening is known for many decades through its beneficial effects on human health. In contrast, sudden exposure to cold, cold shock, is a great risk of cerebral and cardiac injury, especially in the elderly. There is very little published data on the cellular and molecular mechanisms induced by cold adaptation in heart and brain. The aim of this work was to describe and compare different properties heart, liver, brain and brown adipose tissue mitochondria of rats housed at 25 ± 1 řC and at mild cold (9 ± 1 řC, 5 weeks). The high-resolution oxygraphy, spectrophotometry and Western blotting analyses were used. We found differences in the respiratory control between the heart and liver. Cold acclimation decreased activity of the Krebs cycle enzymes. Fatty acid contribution to the respiration reached the maximum in brown fat and the minimum in the hippocampus. However, further study is necessary.
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The role of lipids and ROS in cardioprotective mechanism of chronic hypoxia
Balková, Patricie ; Nováková, Olga (advisor) ; Drahota, Zdeněk (referee) ; Šmíd, František (referee)
The role of lipids and ROS in cardioprotective mechanism of chronic hypoxia Cardiovascular diseases, mainly ischemic heart disease is one of the most frequently cause of morbidity and mortality in developed countries. Therefore effective protection of the heart against ischemia and reperfusion injury is the crucial aim of experimental and clinical cardiology. One of the main streams of cardiovascular research is looking for possibilities of natural heart resistance augmentation. Adaptation to chronic hypoxia is one possibility how to protect the heart against ischemia-reperfusion injury. Chronic hypoxia increases resistance of the myocardium to acute deficiency of oxygen leading to vetricular arrythmias, postischemic contractile dysfunction and necrotic changes in the tissue. Recently, it has been shown that reactive oxygen species (ROS) play an important role in the cardioprotective mechanism of chronic hypoxia. It is known that oxidative stress has a harmful effect in acute ischemia-reperfusion however ROS generated during the adaptation to hypobaric intermittent chronic hypoxia play a role in the induction of cardioprotection. In this study, we demonstrated that adaptation of adult rats to chronic hypoxia increased the activity and protein abundance of manganese superoxide dismutase (MnSOD) in the...
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Ability to Transfer Learned Skills in Activities of Daily Living to Everyday Life in Patients with Acquired Brain Injury; Focusing on patients after stroke
Česáková, Kamila ; Nováková, Olga (advisor) ; Hoidekrová, Kristýna (referee)
Title: Ability to Transfer Learned Skills in Activities of Daily Living to Everyday Life in Patients with Acquired Brain Injury Abstract: The thesis deals with the topic of transfer of skills mainly from the area of activities of daily living learned in therapy to everyday life in patients after stroke. It is a case study within the framework of qualitative research, the main aim of which was to describe the process of transfer of learned skills from the field of ADL to everyday life over a longer period of time. The reason for choosing this topic was that although transfer of learned skills is crucial for rehabilitation and patient return to the home environment, it is still not given enough attention. The aim of the theoretical part was mainly to describe the elements influencing the transfer of learned skills to everyday life and the occupational therapist's possibilities to support this process in therapy and its evaluation. The practical part was conducted with four patients for 6-8 months. For each patient, 6 therapy sessions were first conducted, followed by two visits, each 2-3 months apart, during which a semi-structured interview took place. The aim of the practical part was to test different techniques and approaches in practice as well as to evaluate the transfer afterwards. In particular, the...
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Mapping the Experience of Occupational Therapists with the Entrepreneurship of Occupational Therapy: Challenges and Opportunities
Iroušková, Darina ; Jelínková, Jana (advisor) ; Nováková, Olga (referee)
OF THE DIPLOMA THESIS Name: Bc. Darina Iroušková Thesis supervisor: Mgr. Jana Jelínková Thesis consultant: PhDr. et PhDr. PaedDr. Pavel Harsa, MBA, Ph.D., Ph.D. Title: Mapping the Experience of Occupational Therapists with the Entrepreneurship of Occupational Therapy: Challenges and Opportunities. Abstract of the diploma thesis: The theoretical part of this diploma thesis deals with the elaboration of the topics of entrepreneurship with specifics arising from the fields of health care, occupational therapy, including the conditions of providing health care and the legislative support of business, knowledge about the acquisition of entrepreneurial competencies and addressing ethical and quality issues of services provided. The practical part includes qualitative analysis of in-depth interviews conducted with seven occupational therapists who have established and successfully run private practice. The aim of the research interview was to obtain descriptive information about private occupational therapists' private businesses and to map their personal experiences of entrepreneurship. This exploration results in an understanding of the unique mindset of entrepreneurial occupational therapists by identifying their motives for starting a business and subsequently uncovering the entrepreneurial...
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Verification of fermentative process of grape juice
Procházková, Lenka ; Nováková, Olga (referee) ; Vránová, Dana (advisor)
This thesis deals with identification of yeasts isolated during spontaneous fermentation of grape juice. For analysis the Pinot Noir grape variety grown in the integrated vineyard was chosen. In the theoretical part of this thesis basic information about yeasts are described. Genera of yeasts that occurs during fermentation process and methods based on PCR are also described. In this thesis PCR-RFLP method was used for identification of yeasts. The amplification of the 5,8S-ITS rDNA sequence was performed by the polymerase chain reaction with use of the primers ITS1 and ITS4. The restriction analysis was performed by applying five restriction endonucleases: HaeIII, HinfI, Taq?, AluI, MseI. The amplicons were split into fragments which length and number are typical for the particular species. These fragments were identified by agarose gel electrophoresis and electrophoreograms were evaluated by BioNumerics software. Dendrograms representing genetic similarity of isolated wine yeasts were created by using UPGMA cluster analysis.
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The effect of chronic cold on Ca2+-ATPase (SERCA2) in rat heart
Šeovićová, Maja ; Horníková, Daniela (advisor) ; Nováková, Olga (referee)
Acute cold exposure is a significant stressor activating heat production by shivering after the prolonged exposure cellular oxidative stress increases. Chronic exposure to cold lasting at least 2 weeks leads to the development of cold acclimatization. The main thermogenic role is taken over by non-shivering thermogenesis taking place in brown adipose tissue, which significantly increases its weight due to cold. Cardiac hypertrophy, hypertension and impaired renal function are frequently observed pathologies of acclimatization at 4-5 řC. Our laboratory recently introduced a model of mild chronic cold acclimatization at 8 řC, during which no damage to the heart or kidneys occurs and has proven cardioprotective effect on reducing infarct size. Hence, the influence of this cold acclimatization model on the other cellular and molecular processes needs to be investigated. The cardioprotective effect of cold acclimatization includes changes in β-AR signaling, activation of anti-apoptotic pathways or augmentation of the antioxidant system. The aim of this thesis was to investigate the effect of cold acclimation and subsequent reacclimation on proteins regulating Ca2+ levels in the rat heart (SERCA2 and phospholamban) and on the stimulation of regulatory proteins β-arrestin 1/2 and protein kinase PDPK1. The...
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Analysis of PKCα Influence on Cancer Cell Invasion.
Szabadosová, Emília ; Brábek, Jan (advisor) ; Nováková, Olga (referee)
7 Abstract Protein kinase C alpha (PKCα) is a serine/threonine protein kinase. PKCα is an important protein regulating cell polarity, protein secretion, apoptosis, cell proliferation and differentiation and tumorogenesis. Previous research has shown a role of PKCα also in a cancer cell migration and cancer cell invasion. The aim of this study was to investigate the role of protein kinase C alpha (PKCα) played in amoeboid mode of cancer cell invasion. We showed that higher expression of PKCα resulted in mesenchymal-amoeboid transition of K2 and MDA mesenchymal cancer cell lines, which was accompanied with decreased cancer cell invasive capability in 3D collage matrix. PKCα overexpression had no effect on the cell morphology of A375m2, however, the results showed a trend in increased invasive potential of A375m2 cells. Conversely, the expression of dominant-negative PKCα resulted in amoeboid-mesenchymal transition of A375m2 cells, and it was associated with decreased invasive potential of K2 and MDA cell lines. Furthermore, a linkage between PKCα and phosphatidylinositol 3-kinase (PI3K) was tested. The results revealed that increased activity of PKCα was accompanied with decreased level of active Akt in K2 cell line. To summarize, our results suggest a probable role of PKCα in regulation of amoeboid...
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Occupational therapy in the rehabilitation of patients after fracture of the distal radius and hand
Malečková, Zuzana ; Nováková, Petra (advisor) ; Nováková, Olga (referee)
BACHELOR THESIS ABSTRACT Name, Surname: Zuzana Malečková Supervisor: Mgr. Petra Novotná Consultant: Bc. Aneta Křivánková Title: Occupational therapy in the rehabilitation of patients after fracture of the distal radius and hand Abstract: This is a theoretical-practical work. The theoretical part briefly describes the anatomy and kinesiology of the upper limb with a focus on the hand and wrist, and then presents a summary of the most common fractures in this body part. The last and the longest chapter is about the possibilities of occupational therapy for patients fractures of the distal radius and hand, especially in the period after removal of fixation. Occupational therapy has a very wide application for these patients. Functional disorders of the hand and wrist, which are a frequent complication of fracture treatment, are often closely related to the limitation of self-sufficiency and have a great impact on the patient's quality of life. The aim of the practical part is chosen and verify the occupational therapy methods for people with fractures of the distal radius and hand. This section contains case reports of three patients, two of whom suffered a distal radius fracture and one with fracture of the fifth metacarpal. Each case report contains a detailed initial and discharge examination with a focus...
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Driving Simulator and Its Use in the Rehabilitation of People with Acquired Brain Injury
Strubinská, Šárka ; König, Jan (advisor) ; Nováková, Olga (referee)
DIPLOMA THESIS ABSTRACT Name, surname: Bc. Šárka Strubinská Supervisor: Mgr. Jan König Consultant: Mgr. Eliška Burdová, Mgr. Bc. Jitka Bonková Sýkorová Title: Driving Simulator and Its Use in the Rehabilitation of People with Acquired Brain Injury Abstract: The diploma thesis describes the utilization of a driving simulator for manual control in the rehabilitation of individuals with acquired brain injury. The main objective of this thesis is to determine the feasibility of using a driving simulator and its potential to increase the likelihood of returning to driving by clients with acquired brain injury in the chronic stage. The first sub-objective is to investigate the influence of driving simulator training on attention and the potential to increase the likelihood of returning to driving by clients with acquired brain injury in the chronic stage. The second sub-objective is to determine whether the proposed set of tests is appropriately designed as a criterion for inclusion in the driving simulator research. The theoretical part summarizes the knowledge about acquired brain injury and its consequences, cognitive functions, impairments, car driving, and legislative regulations or possibilities for driving skills testing. Furthermore, this section provides information about rehabilitation by acquired brain...
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