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Ligninolytické enzymy rodu Trametes
Tomšovský, Michal ; Homolka, Ladislav
Eighty-four strains belonging to four species of the genus Trametes Fr. (T. versicolor, T. hirsuta, T. ochracea and T. cervina) collected in different localities and on different substrates were screened for laccase production and eight of them with their monokaryotic derivates were then examined in more detail for their ability to produce also other lignin-modifying enzymes (manganese peroxidase and lignin peroxidase). Production variability was relatively high and no significant correlation was found between the origin of the strains (locality, substrate) and the enzyme production. Dikaryons of all three species (but not of all their strains) exhibited LiP activity, which was not detected in the respective monokaryons.
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Rpg1p/eIF3a se spojuje s mitochondriemi v Saccharomyces cerevisiae
Hašek, Jiří ; Janatová, Ivana ; Špryngar, Martin ; Wolinski, H. ; Kohlwein, S. D.
RPG1/TIF32, the essential gene of Saccharomyces cerevisiae, encodes eIF3a subunit of the translation initiation factor 3. We show here that both temperature sensitive mutant of Rpg1 strongly differ in their distribution at permissive temperature, distribution of the Rpg1-1-GFP fusion is cytosolic, the fusion protein Rpg1-2-GFP was accumulated in large cytosolic aggregates. Using the life image analysis with confocal laser scanning microscope we found that these granules could be inherited to daughter cells and obviously associate with mitochondria.
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Metodika navrhování řasových fotobioreaktorů: matematické modelování hydrodynamického míchání a předpověď parametrů světelného režimu
Papáček, Š. ; Rálek, P. ; Kopecký, Jiří ; Masojídek, Jiří ; Štys, Dalibor ; Petera, K.
Computational fluid dynamics (CFD) and process simulation are important tools for the design and optimisation of biochemical processes. In this contribution, the photobioreactor design methodology based on CFD is proposed. A widely used CFD package (Fluent 6.1, by Fluent Inc.) performs a numerical approximation of microalgae trajectory (pathline) inside tubular photobioreactor. Consequently, prediction of the level of hydrodynamic mixing and corresponding light regime inside the photobioreactor with effective use of strong light by high-density algal culture is possible. In the first approach, the algal suspension is considered as a single-phase incompressible viscous Newtonian fluid, which fills the whole section of the photobioreactor photic zone. Coupling the cell trajectories with the corresponding light conditions, the light histories of single cells are obtained. These records contain complete information about light regime in photobioreactor. Computer simulations for various photobioreactor design parameters and operating conditions allow to establish the quantitative relation between variables representing design and operating parameters and light regime parameters. Thus the problem of optimal PBR design could be resolved.
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