Original title: Construction of unmarked mutants in gene coding for Ser/Thr protein kinases and phosphoprotein phosphatase of Pseudomonas aeruginosa and analysis of theur properties
Translated title: Konstrukce neoznačených mutantů v genech kódujících Ser/Thr proteinkinasy a fosfoproteinfosfatasu Pseudomonas aeruginosa a analýza jejich vlastností
Authors: Nedvědová, Jana ; Lněnička, Petr ; Hercík, Kamil ; Branny, Pavel
Document type: Papers
Conference/Event: European Congress of Clinical Microbiology and Infectious Diseases /14./, Praha (CZ), 2004-05-01 / 2004-05-04
Year: 2004
Language: eng
Abstract: [eng] [cze]
To investigate the importance of individual protein kinases and phosphatases in P. aeruginosa physiology we prepared the single and double mutants in the coresponding genes. Single mutants in protein kinase genes stk1, ppkB and protein phosphatase gene stp1 were prepared by the method of gene replacement with subsequent excision of antibiotic cassettes using Flp-FRT recombination system. Two-dimensional gel electrophoresis was used to analyze the patterns of phosphoproteins obtained in in vitro protein kinase assays with the cell-free lysates of wild type as well as all mutant strains. Abychom zjistili význam jednotlivých proteinkinas a proteinfosfatas Pseudomonas aeruginosa byly připraveny mutanty v jednotlivých genech. Jednoduché mutanty byly připraveny metodou „gene replacement“ a rezistenční kazety byly posléze vyštěpeny pomocí Flp-FRT rekombinačního systému. Pomocí dvourozměrné elektroforézy byly porovnány fosforylační profily divokého typu a mutantů získané in vitro.
Keywords: dna; flp-frt
Project no.: CEZ:AV0Z5020903 (CEP)
Host item entry: European Congress of Clinical Microbiology and Infectious Diseases /14./

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0007912

Permalink: http://www.nusl.cz/ntk/nusl-19279


The record appears in these collections:
Research > Institutes ASCR > Institute of Microbiology
Conference materials > Papers