Original title: Rpg1p/eIF3a associates with mitrochondria in saccharomyces cerevisiae
Translated title: Rpg1p/eIF3a se spojuje s mitochondriemi v Saccharomyces cerevisiae
Authors: Hašek, Jiří ; Janatová, Ivana ; Špryngar, Martin ; Wolinski, H. ; Kohlwein, S. D.
Document type: Papers
Conference/Event: Cytoskeltal Club /12./, Vranovská Ves (CZ), 2004-04-28 / 2004-04-30
Year: 2004
Language: eng
Abstract: [eng] [cze]
RPG1/TIF32, the essential gene of Saccharomyces cerevisiae, encodes eIF3a subunit of the translation initiation factor 3. We show here that both temperature sensitive mutant of Rpg1 strongly differ in their distribution at permissive temperature, distribution of the Rpg1-1-GFP fusion is cytosolic, the fusion protein Rpg1-2-GFP was accumulated in large cytosolic aggregates. Using the life image analysis with confocal laser scanning microscope we found that these granules could be inherited to daughter cells and obviously associate with mitochondria. RPG1/TIF32 je esenciálním genem kvasinky S.cerevisiae. Ukázali jsme, že ts mutace rpg1-1 a rpg1-2 ovlivnují distribuci tohoto proteinu. Zvláště intragenově značená verse mutantu rpg1-2 se akumuluje v granulích i v permisivní teplotě a tyto granule asociují s mitochondriemi a jsou děděny.
Keywords: rpg1; sla2p
Project no.: CEZ:AV0Z5020903 (CEP), GA204/02/1424 (CEP), 2004/29
Funding provider: GA ČR, GA-
Host item entry: Cytoskeletal Club /12./

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0007878

Permalink: http://www.nusl.cz/ntk/nusl-19274


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Research > Institutes ASCR > Institute of Microbiology
Conference materials > Papers