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The use of microsatellite analysis for the characterisation of onion
Mitrová, Katarína ; Ovesná, Jaroslava
The object of the methodology is the process characterizing the autenticity of varieties of onion put through microsatellite analysis (SSR- Single Sequence Repeats) for the characterization of varieties of garlic into the practice. This method allows to identify varieties of onion and characterize genetic resources using DNA markers and determine their genetic similarity based on the length variability of short repeating sequences (microsatellites) in the characterization of varieties of onion, Allium cepa L. The principle of the method is amplification of the genome containing the microsatelite locus by polymerase chain reaction (PCR) using specific primers and subsequent analysis of the lengtht of the products and the identification of the DNA profile to the declared variety.
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The use of microsatellite analysis for the characterisation of garlic
Mitrová, Katarína ; Ovesná, Jaroslava ; Svobodová, Leona
The object of the methodology is the process characterizing the autenticity of varieties of garlic put through microsatellite analysis (SSR- Single Sequence Repeats) for the characterization of varieties of garlic into the practice. This method allows to identify varieties of garlic and characterize genetic resources using DNA markers and determine their genetic similarity based on the length variability of short repeating sequences (microsatellites) in the characterization of varieties of garlic, Allium sativum L. The principle of the method is amplification of the genome containing the microsatelite locus by polymerase chain reaction (PCR) using specific primers and subsequent analysis of the lengtht of the products and the identification of the DNA profile to the declared variety.
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Methodology for evaluation of pest resistance to zoocides by biological methods and antiresistance staregies
Kocourek, František ; Stará, Jitka ; Zichová, Tereza ; Hubert, Jan ; Nesvorná, Marta
The methodology contains description of biological methods for evaluation of pest resistance to zoocides arranged according to world standards. The methods are verified on case studies on pests with proved resistance to insecticides or with high risk of selection of resistance in Czechia, as pollen beetle, Colorado potato beetle, cabbage seedpod weevil, cabbage stem flea beetle and codling moth. The methodology contains survey of basic principles of insect resistance management and proposals of antiresistant strategies for pest mentioned in the case studies. The methods for evaluation of resistance are utilized for resistance monitoring performed by state administration or other organisations. Recommendations given in antiresistant strategies are utilized for pest management in winter rape, potatoes and apples.
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Certifikovaná metodika: Ochrana místních a pomístních jmen a urbanonym jako zdroje lokální, regionální a národní identity a kulturního dědictví
David, Jaroslav ; Mácha, Přemysl
This methodology presents the principal reasons, guidelines, and procedures for the protection of place names as cultural heritage. The methodology first summarizes the general principles of place names conservation and subsequently describes the individual methods of their protection in cities and villages in view of the specific charater of place names and factors which endanger them in different types of settlements. The methodology can be particularly useful for representatives and employees of public and state institutions and local government, or museums, archives and schools, who can significantly contribute to the conservation of the cultural heritage value of place names.
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Methodology of teaching regional history on the example of the Pilsen Region
Mušková, Eva ; Pleskačová, Iva ; Stočes, Jiří ; Zvárová, Jarmila
Methodology of teaching regional history on the example of the Pilsen Region is trying to capture the educational importance of regional history, assess what role regional history teaching plays (should play and could play) in primary schools, and develop an effective way to implement regional themes in the teaching process. The methodology deals with the definition of the region and the approach of this term in historiography. It provides a brief outline of the history of Pilsen historiography completed with a comprehensive account of recent historical literature of regional history of Pilsen and the Pilsen Region, which allows teachers to navigate through the menus historical literature of regional provenance. It further outlines the educational importance of regional history and their role in the current basic education. Besides the theoretical part the methodology also includes project suggestions and guide for realization of an exhibition.
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Methodology for Restoration of the First Republic's Villa Gardens
Zámečník, Roman ; Šimek, Pavel
The aim of the methodology is to create a coherent process aimed at restoring the gardens built between the world wars of the 20th century. The starting point in the methodology was the effort to analyze, interpret and draw attention to this specific segment of national cultural wealth, which has not yet been given greater attention in the professional circles. As a proof of the development of the garden art of the Czech lands, it presents the first republican art, especially its segment - the garden of villas, one of the most vulnerable proofs of the maturity of our culture.
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Methodology for securing and protecting other garden-architectural works that will not capture the project's scope
Zámečník, Roman ; Vaida, Pavel ; Kuťková, Tatiana
The aim of the methodology is to create a methodical approach on the basis of which it is possible to carry out a survey of garden and architectural works that originated between two world wars in the then Czechoslovakia and was not the subject of the project Gardening and Architectural Design in the Context of the National Revival Objectives during the First Czechoslovak Republic. The methodology focuses on a number of specific activities based on the scale and form of the research area of cultural heritage, with the aim of defining the process of identifying, locating, classifying, analyzing, interpreting and preserving information about the area under investigation through sophisticated collection databases. The methodology is based on the underlying premise: what we do not know, we can not protect and preserve it for the next generation. It relies on methods of data processing, evaluation and preservation, which have been verified through the realization of the project Garden-Architectural Creation in the Context of the Implementation of National Revival Objectives during the 1st Czechoslovak Republic.
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Haplotyping of oak populations by chloroplast sequencing reveals origin and homogeneity of populations
Vlasák, Josef ; Cvrčková, H. ; Máchová, P.
The aim of this publication is to present new method of DNA isolation from young\noak leaves that enables efficient amplification of trnD-trnT region of chloroplast\nDNA and direct sequencing of crude PCR reaction mixture. Sequence data of trnDtrnT region have been used to examine Quercus robur and Q. petraea populations variability. High discrimination power of this method is demonstrated, comparable with previously used restriction analysis of four similar chloroplast DNA regions. Variable positions in trnT-trnD chloroplast DNA fragment are mapped and their use for oak haplotyping indicated. As an example, 20 Czech Republic oak populations were analyzed and their history, geografical origin and homo/heterogeneity were revealed.
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