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Sampling of young-of-the-year fishes in rivers. New version
Jurajda, Pavel ; Slavík, O. ; Adámek, Zdeněk ; Janáč, Michal
Metodika je sestavena tak, aby bylo možné s jejím použitím provést odlov, základní zpracování a vyhodnocení vzorku plůdku ryb pro potřeby monitoringu ryb v tocích. Zvolená strategie odběru vzorků musí poskytovat informace o současném stavu rybího společenstva v dané lokalitě. V metodickém návrhu jsou obsaženy základní informace o cílové skupině ichtyofauny, výběru lokalit pro získávání vzorků, vlastním odběru vzorků, jejich zpracování a hodnocení.
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Influence of sampling and fixation on measuring of genome size
Halačka, Karel ; Vetešník, Lukáš ; Fedorčák, J. ; Koščo, J.
Assessment of genome size is an important aspect of many modern studies on fish. Blood is the most common DNA source in fish studies due to its relative ease of sampling and processing. Fish blood, however, precipitates rapidly, leading to clotting. Consequently, anticoagulants (primarily heparin) are added at the time of sampling. Using of increasing heparin concentrations resulted in notable increases in fish erythrocyte genome size readings compared to a non-heparinised control. The differences observed were highly significant, suggesting that the results of previous comparative studies or those determining individual polyploidy may need reinterpretation.
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Settlement of the landscape with European beaver (Castor fiber) in relation to anthropogenic influences
Mikulka, O. ; Patočka, Z. ; Drimaj, J. ; Kamler, J. ; Plhal, R. ; Homolka, Miloslav
The European beaver (Castor fiber) is a protected species in the Czech Republic that has spread to the whole of Moravia. Now, it is a common species whose competitor is only human. The aim of this study was to evaluate the impact of human activity, especially tourism or transport, on the settlement of the landscape by the European Beaver. The area of the protected landscape area Litovelske Pomoravi was chosen to evaluate the settlement of the landscape by the beaver, where the beaver forms a stable population for a long time and the frequented cycling routes, hiking trails and the area are frequently visited by natural floodplain forests. The beaver settlement data were collected by means of the winter monitoring of residential stamps using the GPS system in 2017. Data on the location of cycle routes, hiking trails, human settlements and infrastructure were obtained from available GIS layers. Settlement of European beaver was evaluated in relation to the distance and intensity of interference by tourist activities, traffic or buildings. There were 200 km of watercourses in the monitored area. The inhabited beaver was 39% of the length. The beaver avoided human settlements, preferring the natural environment. The influence of tourism on the beaver settlement has not been proven.
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