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Use of autotrophic algae to production of biomass and some metabolites
Chrástová, Nikola ; Kostovová, Iveta (referee) ; Márová, Ivana (advisor)
Chlorophyll and carotenoids are natural pigments and antioxidants. These pigments are suitable for use in the pharmaceutical, cosmetics or food industries. Lipids are another major metabolite of algae and are a potential feedstock for biofuel production. This bachelor thesis deals with the cultivation of selected genera of autotrophic algae, subsequent isolation of these selected metabolites and their application. The theoretical part focuses on the description of the algae, the metabolites produced, the cultivation and the chosen methods. The experimental part deals with the optimization of the cultivation of algae of the genera Chlorella, Chlamydomonas and Desmodesmus, depending on the chosen medium and intensity of illumination, determination and quantification of algal biomass. Further, extraction of lipidic and carotenoid substances and determination by HPLC and GC was performed. In the overall comparison, the best producer of biomass is the algal genus Desmodesmus and the highest production of pigments and lipid substances was reached in algae of the genus Chlamydomonas.
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Vegetable adaptogens - Schizandra chinensis
Pospíchalová, Lucie ; Půčková, Helena (referee) ; Kotlík, Josef (advisor)
This thesis deals with plant adaptogens (bioactive substances) in the plant Schisandra chinensis. The theoretical part is focused on characterization of the Schisandra genus, characterization of the Schisandra chinensis as a plant, its properties, occurrence and growing possibilities in the regional climate zone of the South Moravian Region as well as the use of this plant. Furthermore, adaptogens are described from a chemical point of view and their possible methods of isolation from individual parts of plants. The experimental part deals with extraction of fruits and leaves from plant Schisandra chinensis. Further, in the leaves extract was determined chlorophyll a, chlorophyll b and carotenoids by spectrophotometry, where the quantity of individual substances was confirmed for months April – May.
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Determination of Chlorophyll Content in Birch and Pine Trees Using Hyperspectral Data
Zachová, Kateřina ; Potůčková, Markéta (advisor) ; Brodský, Lukáš (referee)
The master thesis deals with the determination of the chlorophyll content in birch foliage (Betula pendula Roth) and Scots pine using hyperspectral data. The first part of the thesis concentrates on the literature search dealing with the methods of chlorophyll content in the foliage of selected plant species. In the practical part the emphasis is on the study of spectral reflectance curves and finding their relation to the chlorophyll content from the laboratory determination. Images taken with the hyperspectral sensor HyMap and spectral reflectance curves obtained with the ground ASD FieldSpec 3 spectrometer were available. Using the derived regression model chlorophyll maps were created for Scots pine for three selected locations in the Sokolov coal basin area.
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Specific water properties of pit lakes in the Czech Republic
Hrdinka, Tomáš ; Janský, Bohumír (advisor) ; Benešová, Libuše (referee) ; Herber, Vladimír (referee)
Anthropogenic lakes constitute a significant part of the Czech countryside water component which has not been given sufficient attention so far. The presented thesis deals with the assessment of variability of physico-chemical properties of water in 30 selected pit lakes in order to identify specific features associated with quarrying of different mineral raw materials, basin morphometry and trophic level of the lakes affecting the quality of accumulated water. In the second part of the thesis the author deals with the comprehensive limnological study of the Hromnické Lake with extreme water chemism resulting from excavation of pyritic shales and focuses on the phenomenon of meromixis especially. The results are based on the evaluation of physical properties of water in the lake vertical profile (temperature, conductivity, dissolved oxygen, pH, transparency and colour) and chemical analyzes of water samples collected from the surface and bottom of the lakes during the four seasons in 2003-07 (determination of Ca, Mg, Na, K, Nammon., NO3 - , SO4 2- , Cl- and alkalinity), including determination of chlorophyll-a. In the case study of the Hromnické Lake conducted in 2010-11, the analysis of hydrological regime of the lake, determination of PO4 3- , TOC, selected metals (Fe, Mn, Al, Zn, Ni, Cu, Co,...
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Inter- and intraspecific variability in Vicia faba L. and Zea mays L. response to water deficit
Fridrichová, Lenka ; Holá, Dana (advisor) ; Zelenková, Sylva (referee)
This work monitors the response of plants to water deficit and subsequent restoration of water availability with the aim to expand our knowledge about plant behaviour during and after drought stress from the perspective of two plant species with a different type of photosynthesis. A particular attention was paid to the intraspecific differences and their possible changes during stress and post-stress periods. The plant response was monitored as the changes of selected photosynthetic, morphological and developmental characteristics caused by the cessation of watering for six days followed by a six-day period of renewed optimal water supply. The experiments were carried out on plants with different types of photosynthesis - C3 plant Vicia faba L and C4 plant Zea mays L.; for both plant species, three varieties / genotypes were monitored. The response of both plant species to insufficient water supply differed by the extent of changes in relative water content (RWC), chlorophyll fluorescence parameters, the dry mass of individual leaves (developed during the stress period), the ratio between the aboveground dry mass and dry mass of roots. In drought-stressed plants, downward trend in RWC was observed, as well as the increase of the minimum yield of chlorophyll fluorescence in dark-adapted leaves (F ),...
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Investigation of the impact of stress factors on structure and function of the photosynthetic apparatus of rape (/Brassica napus /L.
RÁKOSNÍKOVÁ, Tamara
The thesis deals with the influence of cold plasma,as a stress factor, on content and fluorescence of chlorrophylls of oilseed rape. Theoretical part deals with characteristic oilseeds rape, fluorescence, photosynthetic pigments and the effects of cold plasma on on physiology of plants. The theoretical part continues in experimental mesurement. The eperiment was done in laboratory conditions. The own of eperiment was to discover the influence of cold plasma on the amount of chlorophylls and fluorescence of chlorophylls. The rape seads were treated with cold plasma for the duration of various time interval. The thesis considersif the plants, which were sprouted from treated seeds, show higher values of physiological activities as compared with plants germinated from conventional seed.
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Measurements and the use of spectral reflectance of solar radiation from surface water levels
Vinciková, H. ; Pechar, L. ; Hanuš, Jan
The methodology describes measurement of spectral reflectance from the surface of water bodies and application of the measured reflectance in assessment and monitoring of water quality especially in terms of the range of eutrophication and development of water blooms. The aim of the suggested methodology is to provide an effective tool for a rapid screening of water quality situation in the Czech Republic, including relatively large areas, and therefore from more water bodies at once. This method using remote sensing allows to gather lot of data on water quality without time-consuming and overall more expensive field sampling and laboratory analysis.
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Vliv nádrže Orlík na kvalitu vody ve Slapské nádrži
Straškrábová, Viera
Přísun fosforu (P) do Slapské nádrže byl hodnocen v letech 1959 – 1960 a v letech 1963 – 1964. Po napuštění nádrže Orlíku se koncentrace P ve Slapech snížila a následně došlo ke snížení letního rozvoje fytoplanktonu, zvláště sinicového vodního květu. Dlouhodobě byly hodnoceny zimní koncentrace fosforu a následný sezónní rozvoj fytoplanktonu. V letech 1960 – 1992 zimní koncentrace P vzrůstaly (vliv zvýšení P v detergentech?), pak opět klesaly. Letní koncentrace chlorofylu se zvyšovaly do 2000, pak se postupně snižují (v důsledku meziroční variability zatím neprůkazné).
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