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The effect of alpha-humulene and carophyllene oxide in ovarian cancer cells
Žáková, Kateřina ; Matoušková, Petra (advisor) ; Svobodová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Žáková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: The effect of alpha-humulene and caryophyllene oxide in ovarian cancer cells. Sesquiterpenes α-humulene and β-caryophyllene oxide are substances with antiproliferative activity against number of tumor cell lines. In this study, the antiproliferative efect of α-humulene and β-caryophyllene oxide was tested alone and in combination with doxorubicin, using neutral red uptake assay. Doxorubicin- sensitive cell line A2780 was more sensitive to the action of doxorubicin and sesquiterpenes than SKOV3 cell line, which is naturally doxorubicin-resistant. To determine the interactions between sesquiterpenes and doxorubicin, software program CalcuSyn which is based on Chou-Talay method was used. The combination indexes ofsesquiterpenes with doxorubicin were determined. Both sesquiterpenes had antagonistic effect on doxorubicin in A2780 cell line and synergistic effect on SKOV3 cell line. The mechanism of the antiproliferative effect of all three tested substances is explained by the production of ROS. For the determination of pro-oxidant effects of the tested substances the DCF assay was used. Our results showed good...
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The effect of alpha-humulene and carophyllene oxide in ovarian cancer cells
Žáková, Kateřina ; Matoušková, Petra (advisor) ; Svobodová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Žáková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: The effect of alpha-humulene and caryophyllene oxide in ovarian cancer cells. Sesquiterpenes α-humulene and β-caryophyllene oxide are substances with antiproliferative activity against number of tumor cell lines. In this study, the antiproliferative efect of α-humulene and β-caryophyllene oxide was tested alone and in combination with doxorubicin, using neutral red uptake assay. Doxorubicin- sensitive cell line A2780 was more sensitive to the action of doxorubicin and sesquiterpenes than SKOV3 cell line, which is naturally doxorubicin-resistant. To determine the interactions between sesquiterpenes and doxorubicin, software program CalcuSyn which is based on Chou-Talay method was used. The combination indexes ofsesquiterpenes with doxorubicin were determined. Both sesquiterpenes had antagonistic effect on doxorubicin in A2780 cell line and synergistic effect on SKOV3 cell line. The mechanism of the antiproliferative effect of all three tested substances is explained by the production of ROS. For the determination of pro-oxidant effects of the tested substances the DCF assay was used. Our results showed good...
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Integration policies in Netherlands and Belgium in comparison with a focus on the situation of Muslim immigrants in these countries
Žáková, Kateřina ; Šánová, Lucie (advisor) ; Fínková, Eva (referee)
The main subject of this bachelor thesis is to compare integration policies in Belgium and the Netherlands, focusing in particular to Muslim immigrants. The values of liberal democracy shared by both countries are underlying their approach to this issue. Research of historical background described in first part of the thesis, was crucial for understanding the way of integration policies course. Rapid economic growth after Second World War was the main reason for arrival of the first Muslim immigrants in the sixties. Wage workers from Turkey and Morocco were hired for the jobs for which local residents showed no interest. First thesis pronouncing the need for a consistent approach to this issue, were formulated almost twenty years later in both countries. After the first oil crisis in the early seventies, the fact that Muslim immigrants want to stay in the countries, despite of lack of work for them, became clear. At the beginning of the eighties both countries are taking the first steps to set up necessary changes. At this point, yet simile policies of examined countries begin to differentiate. The comparative method is used especially in the third part deals with different approaches of Belgium and the Netherlands to this topic. Belgium policy is under the pressure of the tensions between Flemings...
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Ludmila Žofková. Life and works
Žáková, Kateřina ; Koťa, Jaroslav (referee) ; Váňová, Růžena (advisor)
The aim of this piece is to illustrate a life and work of Ludmila Žofková, one of the Czech reformative pedagogy figures of twenties and thirties, 19. century. The introductory part shows us Ms. Žofková's life the way she remembered it in her manuscripts. Next parts are devoted to her pedagogic activity, pedagogic autobiography in other words.Before the Analysis of "Bakov plan" we are going to think up Czech and world pedagogic trends of the beginning of 20. century. The attempt analysis itself is a key part of the piece. It is divided into 3 chapters. In the Didactic analysis we're going to uncover the "Bakov plan" in didactic point of view. The Conditions chapter is showing what conditions the "Bakov plan" realization presumed and what conditions the pedagogues from Bakov faced in real. Last chapter called Educational Aspects and Instruments presents what were the educatory aims the "Bakov plan" had, and which tools were used to make these aims happen. Final part of the piece describes the Czech language education at school in Bakov and Czech language workbooks written by Ludmila Žofková. I believe that this work brings another perspective on knowledge and inspiration to teachers' pedagogic activity.
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Determination of selected parameters in the Czech lager beer
Žáková, Kateřina ; Gregor,, Tomáš (referee) ; Diviš, Pavel (advisor)
The topic of this bachelor thesis is to establish an concentration of selected substances at czech beers lager type. In the first part of the work there are in a range of the theory described basic materials for a beer production and its production proces too. A large part is dedicated to describing of analytical methods used for an analysis of choosen phenolic substances, organic acids and inorganic substances (elements). In the experimental part the measured results are discussed. The target of this work was to compare a composition and to find specific or characteristic ingredients in selected brands type lager produced in Czech republic. Further there were compared the samples with the Czech Beer designation and the samples without those signing.
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Kindergarten
Žáková, Kateřina ; Kavan, Kamil (referee) ; Fišarová, Zuzana (advisor)
It is a new kindergarten in semi developed area. The building is located in Southern Bohemia in the village Strakonice. Kindergarten is divided to two buildings, the garden house and kindergarten. The building has only one floor and a without basement. In object kindergarten is the main building and two individual pavilions. In the main building is office for headmistress of the school, utility room, workshop, cloakroom for employees, store dirty laundry, food warehouses and food preparation and sanitary facilities. From the connecting corridors to get to the individual departments, where you can find children's cloakroom, toilet and washroom for children, isolation room, storage beds and bedding, office for teacher, WC for teacher, store toys and games room and place to relax for children. Pavilion A is the same as pavilion B, only mirrored. The building is based on the footings of plain concrete class C20/25. It is a brick structure of building system called LIVETHERM. Building has a flat roof and roof aisle also with slope 7 ° and 10 . Aisle roof is made of wooden glued trusses. Roofing is made up of modified bitumen belt. Built-up area is 947.6 square meters
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