Název: The co-culture of keratinocytes and fibroblasts on a multi-layered polyester nanofibrous membrane enriched with platelet lysate
Autoři: Blanquer, Andreu ; Filová, Elena ; Jenčová, V. ; Brož, Antonín ; Kuželová Košťáková, E. ; Lisnenko, M. ; Procházková, R. ; Bačáková, Lucie
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Conference on Nanomaterials - Research & Application /13./ NANOCON, Brno (CZ), 20211020
Rok: 2021
Jazyk: eng
Abstrakt: The prevalence of chronic wounds is increasing due to the population ageing and specific illnesses like diabetes mellitus and vascular diseases. Nanofibrous membranes fabricated using synthetic polymers are promising materials to enhance skin wound healing. PCL and PVA membranes are being studied to be used as scaffolds for skin tissue engineering and hydrogels for controlled drug delivery, respectively. The present study considers the development of a multi-layered membrane made of PCL and PVA loaded with platelet lysate (PL). PCL nanofibers allowed cell adhesion and growth, whereas PVA acted as a hydrogel that releases the bioactive compounds of platelet lysate. The cytocompatibility of the membranes containing PL and without it was demonstrated on two cell types involved in wound healing, i.e. keratinocytes and fibroblasts. Both cell types were able to adhere and proliferate on the membranes. In addition, the membrane containing PL enhanced the proliferation of fibroblasts. A co-culture study was also performed by seeding each cell type on one side of the membrane. The cells were co-cultured for 7 days and the results showed that PL increased the proliferation of cells achieving a monolayer of keratinocytes or fibroblasts on each side of the membrane. Thus, the beneficial effect of PCL-PVA+PL membranes on monocultures and co-cultures of skin cells was demonstrated, and these membranes can be considered potential scaffolds for treatment of chronic wounds.
Klíčová slova: electrospun nanofibers; fibroblasts; keratinocytes; platelet lysate; skin wound healing
Číslo projektu: NV18-01-00332 (CEP), 101003407
Poskytovatel projektu: GA MZd
Zdrojový dokument: NANOCON 2021 - Conference proceedings, ISBN 978-80-88365-00-6, ISSN 2694-930X

Instituce: Fyziologický ústav AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na externích webových stránkách.
Externí umístění souboru: https://www.confer.cz/nanocon/2021/read/4364-the-co-culture-of-keratinocytes-and-fibroblasts-on-a-multi-layered-polyester-nanofibrous-membrane-enriched-with-platelet-lysate.pdf
Původní záznam: https://hdl.handle.net/11104/0338714

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-519929


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Fyziologický ústav
Konferenční materiály > Příspěvky z konference