Název: Quest for protein biomarkers of neural stem cells differentiation
Autoři: Skalníková, Helena ; Halada, Petr ; Vodička, Petr ; Motlík, Jan ; Horing, O. ; Jensen, O. N. ; Gadher, S. J. ; Pelech, S. ; Kovářová, Hana
Typ dokumentu: Příspěvky z konference
Konference/Akce: 1st Central and Eastern European proteomic Conference and 3rd Czech Proteomic Conference, Praha (CZ), 2007-10-29 / 2007-10-31
Rok: 2007
Jazyk: eng
Abstrakt: Understanding neurogenesis and neural cell differentiation presents a unique challenge for treatment of nervous system disorders. To gain more insight about molecular mechanism of differentiation of neural cells, we applied different proteomic approaches using classical 2-DE followed by MS and antibody microarrays. Based on 2-DE, profile of constituent proteins of neural stem cells and their differentiated progenies was estabilished at first and then the protein species that are significantly up or down regulated during the differentiation were selected. Differentiation of neural cells was accompanied by changes in the expression of proteins involved in DNA and RNA binding, mRNA processing and transport, stress responses, iron storage and redox regulation. Immunoblot verified changes of hnRNP A1, hnRNP A2/B1, RafB, heme-oxygenasy 2, GRK2 proteins and alphaB-crystallin (S45), CDK1/2 (Y15), PKC mu (S738+S742), proline-rich Akt substrate (T246) phosphorylations during differentiation.
Klíčová slova: cell diferentiation; neurogenesis
Číslo projektu: CEZ:AV0Z50450515 (CEP), CEZ:AV0Z50200510 (CEP), LC545 (CEP), 1M0538 (CEP)
Poskytovatel projektu: GA MŠk, GA MŠk
Zdrojový dokument: 1st Central and Eastern European Proteomic Conference and 3rd Czech Proteomic Conference

Instituce: Ústav živočišné fyziologie a genetiky AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0175381

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-40463


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav živočišné fyziologie a genetiky
Konferenční materiály > Příspěvky z konference