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Original title:
Quest for protein biomarkers of neural stem cells differentiation
Authors: Skalníková, Helena ; Halada, Petr ; Vodička, Petr ; Motlík, Jan ; Horing, O. ; Jensen, O. N. ; Gadher, S. J. ; Pelech, S. ; Kovářová, Hana
Document type: Papers
Conference/Event: 1st Central and Eastern European proteomic Conference and 3rd Czech Proteomic Conference, Praha (CZ), 2007-10-29 / 2007-10-31
Year: 2007
Language: eng
Abstract: Understanding neurogenesis and neural cell differentiation presents a unique challenge for treatment of nervous system disorders. To gain more insight about molecular mechanism of differentiation of neural cells, we applied different proteomic approaches using classical 2-DE followed by MS and antibody microarrays. Based on 2-DE, profile of constituent proteins of neural stem cells and their differentiated progenies was estabilished at first and then the protein species that are significantly up or down regulated during the differentiation were selected. Differentiation of neural cells was accompanied by changes in the expression of proteins involved in DNA and RNA binding, mRNA processing and transport, stress responses, iron storage and redox regulation. Immunoblot verified changes of hnRNP A1, hnRNP A2/B1, RafB, heme-oxygenasy 2, GRK2 proteins and alphaB-crystallin (S45), CDK1/2 (Y15), PKC mu (S738+S742), proline-rich Akt substrate (T246) phosphorylations during differentiation.
Keywords: cell diferentiation; neurogenesis
Project no.: CEZ:AV0Z50450515 (CEP), CEZ:AV0Z50200510 (CEP), LC545 (CEP), 1M0538 (CEP)
Funding provider: GA MŠk, GA MŠk
Host item entry: 1st Central and Eastern European Proteomic Conference and 3rd Czech Proteomic Conference
Institution: Institute of Animal Physiology and Genetics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0175381
Permalink: http://www.nusl.cz/ntk/nusl-40463
The record appears in these collections:
Research > Institutes ASCR > Institute of Animal Physiology and Genetics
Conference materials > Papers
Authors: Skalníková, Helena ; Halada, Petr ; Vodička, Petr ; Motlík, Jan ; Horing, O. ; Jensen, O. N. ; Gadher, S. J. ; Pelech, S. ; Kovářová, Hana
Document type: Papers
Conference/Event: 1st Central and Eastern European proteomic Conference and 3rd Czech Proteomic Conference, Praha (CZ), 2007-10-29 / 2007-10-31
Year: 2007
Language: eng
Abstract: Understanding neurogenesis and neural cell differentiation presents a unique challenge for treatment of nervous system disorders. To gain more insight about molecular mechanism of differentiation of neural cells, we applied different proteomic approaches using classical 2-DE followed by MS and antibody microarrays. Based on 2-DE, profile of constituent proteins of neural stem cells and their differentiated progenies was estabilished at first and then the protein species that are significantly up or down regulated during the differentiation were selected. Differentiation of neural cells was accompanied by changes in the expression of proteins involved in DNA and RNA binding, mRNA processing and transport, stress responses, iron storage and redox regulation. Immunoblot verified changes of hnRNP A1, hnRNP A2/B1, RafB, heme-oxygenasy 2, GRK2 proteins and alphaB-crystallin (S45), CDK1/2 (Y15), PKC mu (S738+S742), proline-rich Akt substrate (T246) phosphorylations during differentiation.
Keywords: cell diferentiation; neurogenesis
Project no.: CEZ:AV0Z50450515 (CEP), CEZ:AV0Z50200510 (CEP), LC545 (CEP), 1M0538 (CEP)
Funding provider: GA MŠk, GA MŠk
Host item entry: 1st Central and Eastern European Proteomic Conference and 3rd Czech Proteomic Conference
Institution: Institute of Animal Physiology and Genetics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0175381
Permalink: http://www.nusl.cz/ntk/nusl-40463
The record appears in these collections:
Research > Institutes ASCR > Institute of Animal Physiology and Genetics
Conference materials > Papers
Record created 2011-07-01, last modified 2024-01-26