Název: Temperature-programed micro-HPLC analysis of fatty acid methyl esters with APCI-MS detection
Autoři: Vrkoslav, Vladimír ; Rumlová, Barbora ; Cvačka, Josef
Typ dokumentu: Příspěvky z konference
Konference/Akce: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Rok: 2018
Jazyk: eng
Abstrakt: HPLC/APCI-MS analysis at microliters-per-minute flow rates was optimized for separation of fatty acid methyl esters (FAMEs). HPLC C18 column with an internal diameter of 0.3 mm and isocratic elution using 99.9 % acetonitrile and 0.1% formic acid were employed. Standard APCI ion source was suitable for detection of FAMEs at 10 μl/min flow rate with the detection limit of micrograms-per-milliliter. APCI-MS spectra with predominant [M + H]+ molecular adducts were observed. The main advantage of micro-flow measurements is the possibility of using a temperature gradient, which significantly reduces retention times of FAMEs with longer aliphatic chain. The significant reduction of solvent consumption is also an important economic and environmental advantage. The positions of double bonds in FAME chains were established using acetonitrile-related adducts and tandem mass spectrometry. The optimized method was applied for analysis of FAMEs in triacylglycerol fraction of black currant seeds oil.
Klíčová slova: atmospheric pressure chemical ionization; liquid chromatography; mass spectrometry
Číslo projektu: GA16-01639S (CEP)
Poskytovatel projektu: GA ČR
Zdrojový dokument: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1

Instituce: Ústav organické chemie a biochemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na externích webových stránkách.
Externí umístění souboru: http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Původní záznam: http://hdl.handle.net/11104/0291136

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-390683


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav organické chemie a biochemie
Konferenční materiály > Příspěvky z konference