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Original title:
Temperature-programed micro-HPLC analysis of fatty acid methyl esters with APCI-MS detection
Authors: Vrkoslav, Vladimír ; Rumlová, Barbora ; Cvačka, Josef
Document type: Papers
Conference/Event: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Year: 2018
Language: eng
Abstract: HPLC/APCI-MS analysis at microliters-per-minute flow rates was optimized for separation of fatty acid methyl esters (FAMEs). HPLC C18 column with an internal diameter of 0.3 mm and isocratic elution using 99.9 % acetonitrile and 0.1% formic acid were employed. Standard APCI ion source was suitable for detection of FAMEs at 10 μl/min flow rate with the detection limit of micrograms-per-milliliter. APCI-MS spectra with predominant [M + H]+ molecular adducts were observed. The main advantage of micro-flow measurements is the possibility of using a temperature gradient, which significantly reduces retention times of FAMEs with longer aliphatic chain. The significant reduction of solvent consumption is also an important economic and environmental advantage. The positions of double bonds in FAME chains were established using acetonitrile-related adducts and tandem mass spectrometry. The optimized method was applied for analysis of FAMEs in triacylglycerol fraction of black currant seeds oil.
Keywords: atmospheric pressure chemical ionization; liquid chromatography; mass spectrometry
Project no.: GA16-01639S (CEP)
Funding provider: GA ČR
Host item entry: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1
Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Original record: http://hdl.handle.net/11104/0291136
Permalink: http://www.nusl.cz/ntk/nusl-390683
The record appears in these collections:
Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers
Authors: Vrkoslav, Vladimír ; Rumlová, Barbora ; Cvačka, Josef
Document type: Papers
Conference/Event: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Year: 2018
Language: eng
Abstract: HPLC/APCI-MS analysis at microliters-per-minute flow rates was optimized for separation of fatty acid methyl esters (FAMEs). HPLC C18 column with an internal diameter of 0.3 mm and isocratic elution using 99.9 % acetonitrile and 0.1% formic acid were employed. Standard APCI ion source was suitable for detection of FAMEs at 10 μl/min flow rate with the detection limit of micrograms-per-milliliter. APCI-MS spectra with predominant [M + H]+ molecular adducts were observed. The main advantage of micro-flow measurements is the possibility of using a temperature gradient, which significantly reduces retention times of FAMEs with longer aliphatic chain. The significant reduction of solvent consumption is also an important economic and environmental advantage. The positions of double bonds in FAME chains were established using acetonitrile-related adducts and tandem mass spectrometry. The optimized method was applied for analysis of FAMEs in triacylglycerol fraction of black currant seeds oil.
Keywords: atmospheric pressure chemical ionization; liquid chromatography; mass spectrometry
Project no.: GA16-01639S (CEP)
Funding provider: GA ČR
Host item entry: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1
Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Original record: http://hdl.handle.net/11104/0291136
Permalink: http://www.nusl.cz/ntk/nusl-390683
The record appears in these collections:
Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers
Record created 2019-01-07, last modified 2021-11-24