Název:
Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides
Autoři:
Čáslavský, Josef ; Chmelík, Josef Typ dokumentu: Příspěvky z konference Konference/Akce: Informal meeting on mass spectrometry /18./, Praha (CZ), 2000-04-30 / 2000-05-04
Rok:
2000
Jazyk:
eng
Abstrakt: MALDI-TOF MS has been successfully applied for the analysis of a wide range of organic biopolymers including oligosaccharides. We focus our attention to the group of oligosaccharides occuring in beer and in malting and mashing products, which are mainly oligosaccharides containing from 3 to more than 30 glucose units in their molecules. We tried to evaluate various factors influencing the obtained results. We found that the shape of the spectrum depends significantly on the energy of ionising laser pulse and also on the amount of the analyte on the MALDI target. These effects were studied with standard mixtures as well as with real samples.
Klíčová slova:
hmotnostní spektrometrie; oligosacharidy; MALDI-TOF; mass spectrometry; oligosaccharides Číslo projektu: ERB IC-15-CT98-0909, CEZ:AV0Z4031919 (CEP) Poskytovatel projektu: COPERNICUS Zdrojový dokument: Proceedings of the 18th Informal Meeting on Mass Spectrometry, ISBN 80-238-5344-9
Instituce: Ústav analytické chemie AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0059659