Original title: Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides
Authors: Čáslavský, Josef ; Chmelík, Josef
Document type: Papers
Conference/Event: Informal meeting on mass spectrometry /18./, Praha (CZ), 2000-04-30 / 2000-05-04
Year: 2000
Language: eng
Abstract: MALDI-TOF MS has been successfully applied for the analysis of a wide range of organic biopolymers including oligosaccharides. We focus our attention to the group of oligosaccharides occuring in beer and in malting and mashing products, which are mainly oligosaccharides containing from 3 to more than 30 glucose units in their molecules. We tried to evaluate various factors influencing the obtained results. We found that the shape of the spectrum depends significantly on the energy of ionising laser pulse and also on the amount of the analyte on the MALDI target. These effects were studied with standard mixtures as well as with real samples.
Keywords: MALDI-TOF; mass spectrometry; oligosaccharides; hmotnostní spektrometrie; oligosacharidy
Project no.: ERB IC-15-CT98-0909, CEZ:AV0Z4031919 (CEP)
Funding provider: COPERNICUS
Host item entry: Proceedings of the 18th Informal Meeting on Mass Spectrometry, ISBN 80-238-5344-9

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0059659

Permalink: http://www.nusl.cz/ntk/nusl-24539


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers