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A simple method for detection of Czech bolting garlics (Allium sativum L.) using microsatellite analysis: methodology for practice
Ovesná, Jaroslava ; Mitrová, Katarína ; Kučera, Ladislav
The aim of the methodology was to verify and compile a set of microsatellite markers for the purpose of verification to be used for the undisputed identification of selected types of Czech bolting garlics. The object of submitted methodology is to simplify procedure that allow to determine Czech bolting garlic using microsatellite analysis (SSR- Single Sequence Repeats), which is applicable in testing laboratories. The described method allows to identify the variety and breeding material of bolting garlic of Czech origin using DNA markers (microsatellite alleles) that are present only for this group of varieties and breeding lines. By the amplification of the genome containing the microsatellite locus by polymerase chain reaction (PCR) using specific, fluorescently labeled primers, and subsequent analysis of the lengths of PCR products. Methodology newly brings a unique set of diagnostic SSR markers, describe the evaluation and interpretation of the results.
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Methodology for detecting the presence of cranberries and lingonberries in processed products using molecular SSR markers: methodology for practice
Kučera, Ladislav ; Ovesná, Jaroslava
The subject of the methodology is a procedure for diagnosing the presence of lingonberries (Vaccinium vitis-idaea L.) and cranberries (Vaccinum macrocarpon L.) in processed products such as tea or fruit ingredients of many food products (jams, jelly, purree, tea). The methodology exploit DNA sequences that are typical for each plant species. Thus, molecular DNA markers that characterize the plant species by SSRs (single sequence repeats) are used. The methodology describes the extraction of DNA from the assumed matrix, the use of selected SSR markers and the analytical data evaluation procedure. The method can be used in any solid equipped molecular-genetic laboratory. The method is applicable for the characterization of DNA isolated from any part of the plant, fruit, and derived food or food supplements. Method describes the procedure and necessary equipment to perform the analysis. Protocol recommends how to evaluate the results.
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Isolation and characterization of laccase gene in barley(Hordeum vulgare).
Drábková, Lenka ; Kučera, Ladislav (advisor) ; Čurn, Vladimír (referee) ; Fischer, Lukáš (referee)
Laccases are one of the oldest enzymes ever studied. For the first time laccase was mentioned by Yoshida in 1883 (Yoshida H, 1883). Laccases (EC 1.10.3.2, p-diphenol-O2 oxidoreductase), are copper-containing enzymes and are part of a larger group of enzymes termed polyphenol oxidase. Laccases catalyze oxidation of a broad range of substrates e.g., polyphenols, substituted phenols, diamines, but also some anorganic compounds with the concomitant reduction of oxygen to water. The source of electrons in these redox reactions are usually four copper atoms. Fungal laccases, so far the most extensively studied group, are known for their roles in lignin degradation, bioremediation, morphogenesis and pathogenicity. Despite many years of research, the physiological function of laccases in plants remains largely unknown. Plant laccase have been proposed to be involved in lignification. It participates mainly in the synthesis of lignin, maintenance of the cell wall integrity and structure and wound healing. Laccases can catalyze a wide variety of enzymatic reactions and apparently have a role in plants response to stress. Plant laccases have been described especially in Arabidopsis (Arabidopsis thaliana), corn (Zea mays), wheat and rice (Oryza sativa). Laccases have not been yet characterized in any others...
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The sampling methodology for GM plants unintentionally present in the environment
Ovesná, Jaroslava ; Kučera, Ladislav ; Sovová, Tereza ; Mitrová, Katarína ; Pouchová, Vladimíra
In this methodology, sampling of plants from agricultural crops(corn, soybean, canola and potato) is defined to assess the presence of unauthorized GM plants in the environment.The methodology is applied mainly in the field of inspection controls of the compliance with Act no.78/2004. The sampling and collected samples must represent to a maximum extent the crops of the controlled part of the field (land parcel or land parcel portion). The basic applications of the methodology include:(a) low portion of unauthorized GM plants(seed contaminated with GM plants unauthorized for cultivation), (b) monitoring of the presence of unauthorized GM plants on land parcel, where there was such an occurence recorded in previous years (for example, plants growing on the field from seeds or tubers from the so called "soil´s seed bank").
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Zabezpečení laboratorní detekce geneticky modifikovaných organismů
Výzkumný ústav rostlinné výroby‚ Praha-Ruzyně ; Koucká, Petra ; Hodek, Jan ; Pouchová, Vladimíra ; Kučera, Ladislav ; Ovesná, Jaroslava
Podklady pro metodiku vzorkování rostlinných materiálů pro potřeby správních úřadů. Zpracování informací z ENGL pro potřeby ČIŽP. Shromažďování referenčních materiálů. Metodiky stanovení GMO. Svazek obsahuje sborník na téma Geneticky modifikované organismy - současnost, rozšíření a možné interakce s životním prostředím. Jednotlivé příspěvky ve sborníku: geneticky modifikované organismy - základní informace, Možnosti stanovení GMO, Křižitelnost plodin a planých druhů jako potenciál pro únik genetických modifikací, Šíření pylu v porostech lnu setého, Problematika GM hrachu v ČR, Perspektivy využití GMO, Problematika GM bramboru v ČR.
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