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Stress resistance of PHA accumulating bacteria against conditions associated with gastrointestinal tract
Kovářová, Radka ; Nováčková, Ivana (referee) ; Obruča, Stanislav (advisor)
This bachelor thesis is focused on stress resistance of PHA accumulating bacteria against unfavourable conditions associated with gastrointestinal tract. Two bacterial strains were chosen as model microorganisms for this purpose. Cupriavidus necator H16 producing PHA and its mutant strain Cupriavidus necator PHB-4 without the ability of PHA accumulation. Firstly, three gastrointestinal juices of different concentrations were chosen for the experimental part, namely gastric, bile and pancreatic juices. The stressed bacterial strain was then determined using a spread plate method and a flow cytometry. Other methods used include antimicrobial tests. The agar diffusion method was first tested to determine the size of the inhibition zones. The last method that has been performed is the broth dilution method, which serves to compare the absorbance of pure bacterial suspensions and stressed suspensions with the given juice of a certain concentration. The results show that the production of PHA represents a disadvantage in context of conditions associated with gastrointestinal tract of accumulating bacterial strain.
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Entry of the Nobilis Tilia Co., Ltd to the German Beauty Industry
Dudrová, Lenka ; Kovářová, Radana (referee) ; Zich, Robert (advisor)
Hlavnou témou mojej diplomovej práce je zhodnotenie, či má firma Nobilis Tilia, s.r.o. rozšíriť svoje podnikateľské aktivity na zahraničné trhy, konkrétne na nemecký trh s kozmetikou. Celá práca je postupne rozdelená do troch hlavných častí – na teoretickú, analytickú a návrhovú. V teoretickej časti sú zhodnotené poznatky získané z literárnych zdrojov zameraných na strategický management firiem. Analytická časť sa zaoberá firmou Nobilis Tilia, s.r.o., jej históriou a portfóliom produktov. Následne je spracovaná analýza vonkajšieho obecného prostredia, Porterova Analýza Piatich Síl, SWOT analýza nemeckého trhu s kozmetikou. Návrhová časť práce ponúka prehľad odporúčaní, založených na výsledkoch spomínaných analýz.
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Biotechnological production of PHA copolymers containing 4-hydroxybutyrate
Kovářová, Radka ; Pernicová, Iva (referee) ; Obruča, Stanislav (advisor)
The proposed diploma thesis aims to study the biotechnological production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer. The subject of the experimental part was first to select a suitable bacterial strain from five selected microorganisms with different carbon precursors applied at various concentrations. The five selected microorganisms used in the experimental part include bacterial strains Cupriavidus malaysiensis DSM 19416, DSM 19379, and DSM 25816. Furthermore, the strain Thermomonas hydrothermalis DSM 14834 and Aneurinibacillus thermoaerophilus H1 CCM 8960. The experiment shows that the most suitable candidate for biotechnological production is the bacterial microorganism Cupriavidus malaysiensis DSM 19379. Finally, the biotechnological production of the copolymer was investigated utilizing a batch cultivation technique in a laboratory bioreactor.
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Kinds and sources of financing of living
Zuzaňáková, Markéta ; Kovářová, Renata (referee) ; Hrabincová, Dagmar (advisor)
This bachelor thesis deals with the characteristics of housing and financial sources for housing. It is divided into two parts, theoretical and practical. The theoretical part includes information about the housing fund of the Czech Republic and about the financial sources for housing, especially mortgage credit, loans from building society account and loans provided by the State fund for housing development. The practical part includes a description of three model examples using a mortgage credit to buy a new apartment. The aim of this paper is to introduce the basic terminology related to housing, forms, financial sources for housing and their application to specific examples.
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Effect of physiotherapy in Puerperium on the Recovery of women after Caesarean Section
Kovářová, Radka ; Honců, Pavla (advisor) ; Budská, Nikol (referee)
1 ABSTRACT The main objective: Caesarean section is currently the most frequently performed obstetric operation and probably the most common abdominal operation at all. The condition after SC is a combination of the condition after pregnancy and the condition after major abdominal surgery. Nevertheless, physiotherapy is not provided as standard in all maternity hospitals in the Czech Republic. Physiotherapy is also not normally indicated after discharge from the hospital. The aim of the present study was to prove that physiotherapy in puerperium influences the recovery of women after SC. Methods: At the beginning, 57 women participated in the study. Data from 45 women (20 women in the treatment group and 25 women in the control group) advanced to further evaluation. During the six weeks postpartum period, the women in TS completed self-therapy consisting of post-operative wound care and rehabilitation exercises. Study participants from KS did not receive a self-therapy plan or other instructions regarding post-operative wound care beyond the information given to them by maternity staff or which they sought out themselves. Data collection took place in the form of a questionnaire in the period 06/2021-03/2022. The women evaluated 8 recovery parameters on the VAS in four monitored periods: 2-3 days after...
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Role of school by idientification and support of matematicaly gifted pupils
Kovářová, Renata ; Stejskal, Bohumil (advisor) ; Hájková, Vanda (referee) ; Kysučan, Jaroslav (referee)
Contemporary period and situation puts a lot of demands on individual members of the society, especially on their individual using skills and successfulness from one's childhood to adulthood. That is the reason why every society can't risk the potential of its people. Also Czech representatives became aware of this situation. To support talents and abilities of people lots of new projects are arising, f.e. Project Czech Head ("Projekt Česká hlava") which has the part called Little Heads ("Projekt České hlavičky"), organized since 2007 for Czech high-school students of technical and natural science branches. In co- operation of the Czech Senate and Parliamen chairman is also announced the competition for european students called Innovating Minds (since 2008). All the professionals who deal with this problem are aware of the fact that the number of successful members of society raise due to effective support and education of children since the early childhood. Pupils with the exceptional talents, according to the valid Czech law, are placed and classified as the pupils with special needs. Our contemporary trend in education of pupils and students with special needs is integration in possible way in dependence of the real needs and demands. Of course, the same law is valid and belongs to extraordinary...
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Biotechnological production of PHA copolymers containing 4-hydroxybutyrate
Kovářová, Radka ; Pernicová, Iva (referee) ; Obruča, Stanislav (advisor)
The proposed diploma thesis aims to study the biotechnological production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer. The subject of the experimental part was first to select a suitable bacterial strain from five selected microorganisms with different carbon precursors applied at various concentrations. The five selected microorganisms used in the experimental part include bacterial strains Cupriavidus malaysiensis DSM 19416, DSM 19379, and DSM 25816. Furthermore, the strain Thermomonas hydrothermalis DSM 14834 and Aneurinibacillus thermoaerophilus H1 CCM 8960. The experiment shows that the most suitable candidate for biotechnological production is the bacterial microorganism Cupriavidus malaysiensis DSM 19379. Finally, the biotechnological production of the copolymer was investigated utilizing a batch cultivation technique in a laboratory bioreactor.
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Entry of the Nobilis Tilia Co., Ltd to the German Beauty Industry
Dudrová, Lenka ; Kovářová, Radana (referee) ; Zich, Robert (advisor)
Hlavnou témou mojej diplomovej práce je zhodnotenie, či má firma Nobilis Tilia, s.r.o. rozšíriť svoje podnikateľské aktivity na zahraničné trhy, konkrétne na nemecký trh s kozmetikou. Celá práca je postupne rozdelená do troch hlavných častí – na teoretickú, analytickú a návrhovú. V teoretickej časti sú zhodnotené poznatky získané z literárnych zdrojov zameraných na strategický management firiem. Analytická časť sa zaoberá firmou Nobilis Tilia, s.r.o., jej históriou a portfóliom produktov. Následne je spracovaná analýza vonkajšieho obecného prostredia, Porterova Analýza Piatich Síl, SWOT analýza nemeckého trhu s kozmetikou. Návrhová časť práce ponúka prehľad odporúčaní, založených na výsledkoch spomínaných analýz.
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Stress resistance of PHA accumulating bacteria against conditions associated with gastrointestinal tract
Kovářová, Radka ; Nováčková, Ivana (referee) ; Obruča, Stanislav (advisor)
This bachelor thesis is focused on stress resistance of PHA accumulating bacteria against unfavourable conditions associated with gastrointestinal tract. Two bacterial strains were chosen as model microorganisms for this purpose. Cupriavidus necator H16 producing PHA and its mutant strain Cupriavidus necator PHB-4 without the ability of PHA accumulation. Firstly, three gastrointestinal juices of different concentrations were chosen for the experimental part, namely gastric, bile and pancreatic juices. The stressed bacterial strain was then determined using a spread plate method and a flow cytometry. Other methods used include antimicrobial tests. The agar diffusion method was first tested to determine the size of the inhibition zones. The last method that has been performed is the broth dilution method, which serves to compare the absorbance of pure bacterial suspensions and stressed suspensions with the given juice of a certain concentration. The results show that the production of PHA represents a disadvantage in context of conditions associated with gastrointestinal tract of accumulating bacterial strain.
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