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Detection and genotypisation of Helicobacter pylori strains in Waldeyers lymphatic tissue and its relationship to the formation of pathologies in this area
Nártová, Eva ; Astl, Jaromír (advisor) ; Smilek, Pavel (referee) ; Slípka, Jaroslav (referee)
The aim ofthis study was to reveal the presence ofHelicobacter pylori (HP) in the Waldeyers lymphatic tissue in the group ofchildren and adults, along with its possible role in the etiology of tonsillar carcinoma and benign diseases (chronic tonsillitis, OSAS, adenoids). In our study we have confirmed the hypothesis that HP is presented m the Waldeyers lymphatic tissue. as well as in the stomach and that the oropharynx and epipharynx are the an extragastric reservoir ofHP. Mucosa associated lymphatic tissue in the stomach is similar to lymphatic tissue ofWaldeyer ring. These conditions can be very favourable for the survival ofHP and thus can promote inflammation changes and immune changes as well as in the stomach. ln our study, using the real-time PCR method we have detected high incidence ofHP DNA in adenoids and tonsillar tissue. ln the group ofbenign diseases, the most frequent genotypes were CagA VacAsIbm1 and CagA-VacAslbm2. In the group of patients with tonsillar carcinoma, the most frequent genotype was CagA-VacAslbml. Genotyping identified strains ofHP showed differences in comparison with the predominant strains which are most frequently found in the stomach. Genotypic analysis ofI-IP strains showed that the less prevalent virulent strains ofHP, known as cagA negative and vacA positive....
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NO Synthases, TGF-beta 1 and EGF Expression in Patients with Proven Oropharyngeal Helicobacter Pylori Infection and its Role in Oropharyngeal Pathogenesis
Lukeš, Petr ; Astl, Jaromír (advisor) ; Šlapák, Ivo (referee) ; Fučíková, Terezie (referee)
The thesis focuses on detection of Helicobacter pylori (HP) in the oropharyngeal lymphatic tissue and changes of selected cytokines expression according to the inflammatory response to the HP infection. HP is well known gastric pathogen, which leads to the development of chronic gastritis, gastric ulcers and malignancies such as gastric carcinoma or MALT lymphoma. According to the supposed way of transmission of this bacterium, oral cavity was investigated for HP presence recently. Data about possible colonisation of oropharyngeal tissue are insufficient. Several papers were published with inconsistent results. This inconsistency is due to different investigation methods used. The pathogenetic effect of HP in the oropharyngeal tissue was not investigated. It is supposed, that HP could lead to inflammation or carcinogenesis of oropharyngeal tissue in the same way as in gastric mucosa. The presence of HP in oropharyngeal tissue samples of patients suffering from tonsillar carcinoma, chronic tonsillitis or obstructive sleep apnea syndrome (OSAS) was investigated in this study. Real-time PCR was used. Part of the patients was investigated for gastric HP presence using UBT and PCR. Specific anti-HP antibodies and levels of EGF and TGF-beta 1 were detected in sera. Differences in iNOS, eNOS and caspase-3 were...
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Molecular genetic examinations in clinically defined group of patients with syndromic sight and hearing impairment in rare genetic disorders associated with deafblindness in the CR and SR
Čopíková, Jana ; Kremlíková Pourová, Radka (advisor) ; Astl, Jaromír (referee) ; Baxová, Alice (referee)
Deafblindness is a combined impairment of vision and hearing with an incidence of about 1: 8000 children and 1: 5500 adults. The most common genetic causes are the Stickler (STL) and Usher (USH) syndromes. The main goal of this work is to provide an up-to-date overview of STL and USH in the Czech and Slovak Republic (CR and SR), to determine the correlations between the genotype and phenotype in our population and the associated diagnostic criteria. Using sequencing and MLPA we examined 45 patients from 28 families for suspected STL. We found potentially causal variants of STL genes in 39 patients from 22 families. Fifteen different COL2A1 variants (8 being novel) were found in 28 patients from 18 families and 4 novel COL11A1 variants were found in 11 patients from 4 families. We identified the cause of the disease in 79 % of the families. The USH study involved 30 patients from 27 families. The most frequent cause was USH2A pathogenic variants, i.e. 19 variants in 14 families, 9 being novel. Less common were pathogenic variants in MYO7A (6 variants in 3 families, 5 being novel), USH1C and CDH23 (3 variants, 2 being novel, in 2 families both) genes. In 2 families, compound heterozygosity was found for variants in two different USH genes. The deafblindness etiology was clarified for 24 patients from...
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Detection and genotypisation of Helicobacter pylori strains in Waldeyers lymphatic tissue and its relationship to the formation of pathologies in this area
Nártová, Eva ; Astl, Jaromír (advisor) ; Smilek, Pavel (referee) ; Slípka, Jaroslav (referee)
The aim ofthis study was to reveal the presence ofHelicobacter pylori (HP) in the Waldeyers lymphatic tissue in the group ofchildren and adults, along with its possible role in the etiology of tonsillar carcinoma and benign diseases (chronic tonsillitis, OSAS, adenoids). In our study we have confirmed the hypothesis that HP is presented m the Waldeyers lymphatic tissue. as well as in the stomach and that the oropharynx and epipharynx are the an extragastric reservoir ofHP. Mucosa associated lymphatic tissue in the stomach is similar to lymphatic tissue ofWaldeyer ring. These conditions can be very favourable for the survival ofHP and thus can promote inflammation changes and immune changes as well as in the stomach. ln our study, using the real-time PCR method we have detected high incidence ofHP DNA in adenoids and tonsillar tissue. ln the group ofbenign diseases, the most frequent genotypes were CagA VacAsIbm1 and CagA-VacAslbm2. In the group of patients with tonsillar carcinoma, the most frequent genotype was CagA-VacAslbml. Genotyping identified strains ofHP showed differences in comparison with the predominant strains which are most frequently found in the stomach. Genotypic analysis ofI-IP strains showed that the less prevalent virulent strains ofHP, known as cagA negative and vacA positive....
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