National Repository of Grey Literature 75 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Applying Sensory Profile 2 Test with Premature Children from the occupational therapist's point of view. Subtitle: The influence of premature birth on sensory integration processing of 2 year old children.
Ředinová, Barbora ; Haškovcová, Eliška (advisor) ; Nováková, Olga (referee)
This diploma thesis deals with the problematics of sensory processing with premature children. The aim of the thesis is to show the important role of an occupational therapist taking care of infants with perinatal risks already at NICU. Another aim of the thesis is to create an unofficial Czech translation of the standardized Toddler Sensory Profile 2 Test and use it to evaluate the quality of sensory processing with premature children and compare the results with those of children born in term. In the theoretical part, the classification of new- borns is described for better understanding and orientation, together with the risks premature birth brings and the role of an occupational therapist taking care of such children. In the thesis, the approach of Sensory Integration and a classification of Sensory Processing Disorders (SPD) are described. At the end of the theoretical part, the Sensory Profile 2 Test is further described, especially its part dedicated to toddlers (TSP2). The practical part is made of a quantitative research. In the research, the total number of 70 two-year-old toddlers were assessed using TSP2, out of which 44 were born premature and 26 in term. The group of premature toddlers was further divided into 15 late preterm, 17 very preterm and 17 extreme preterm children. The...
Studies on the molecular mechanisms of cardioprotective effects of morphine
Škrabalová, Jitka ; Novotný, Jiří (advisor) ; Nováková, Olga (referee) ; Hlaváčková, Markéta (referee)
Acute and chronic morphine administration can significantly reduce ischemia- reperfusion injury of the rat heart. However, the molecular mechanisms mediating the protective effect of morphine are not yet fully elucidated. Concurrently, there is a lack of information about the effects of the long-term action of morphine on heart tissue. Therefore, in the first part of the project, we studied the effect of long-term administration of high doses of morphine (10 mg/kg/day, 10 days) on rat heart tissue. In the second part of the project, we investigated the effect of 1 mM morphine on viability and redox state of rat cardiomyoblast cell line H9c2 that was influenced by oxidative stress elicited by exposure to 300 μM tert-butyl hydroperoxide (t-BHP). Our experiments have shown that long-term morphine administration affected neither the amount nor the affinity of myocardial β-adrenergic receptors (β-AR), but almost doubled the number of the dominant isoforms of myocardial adenylyl cyclase (AC) V/VI and led to supersensitization of AC. At the same time, proteomic analyses revealed that long-term morphine administration was associated with significant changes in the left ventricular proteome. In particular, there was an increase in the expression of heat shock proteins (HSP). Increased expression of HSP27...
G pretein-couplad receptors in membrane domains
Rudajev, Vladimír ; Svoboda, Petr (advisor) ; Nováková, Olga (referee) ; Teisinger, Jan (referee)
Conclusions our purposewastoseparďeplasmamembranefractioncharacterisedaslipid rafu (or mernbranedomains)andítndtheextentofG protein coupledreceptors(GPCRs) raft association.Threediffererrttechniqueswere applied- detergontextraction,alkaline treaÍnent wift sonicďion,andhomogenisation'We foundtha on|yTriton X.l00 treatmentledto lipid raffs separďon from bulk membranephase.Many markermoleculeshelpedus to characterise low (sucrose|5.25%)andhigh-density(sucrose40%)membranefractionsseparďedon sucrosederrsitygraďents after cells homogenisation'ďkďine or detergentextraction.Low density,dercrgentresisiantmembranefragnents, were characterisedby presenceof mernbranedomainsproteinscaveolin,flotillin, andGPl-boundalkalinephosphatase,CD55, CD58, andCD59, whereashigh-densitysucrosegradientfractionwereenrichedin non-raft proteinsNA/K-ATPase' MHC l' cDl47' calnexinandtrarsferrinerec€ptoÍ'Using alkaline extractionandhomogenisation(detergent-freemethods)ďd notprovide membranedomains separatedfromnon.raflphaseofplasmamembrane. Inadditiontovariousmarkerproteins,gradientdistributionsof GPCRs andtrimericG proteinGso, GqďGl lo', Gil,2a andGp wereanalysedaswell' Innon.deteÍgent prepaÍations,all monitoredproteinsco.localísedin gradientfractionsrepresenting35-4070 sucÍose.on theotherhand,appIicationof TritonX. l 00 enabledus to...
Organization of myofibrillar PCr/CK system in skeletal muscle
Žurmanová, Jitka ; Mejsnar, Jiří (advisor) ; Nováková, Olga (referee) ; Neckář, Jan (referee)
5. SUMMARYOFRESULTES Áll resultshavebeenpublishedor acceptedin joumalswith IF. Ite list of publicďionis enclosedin thechaoter6. 1) Lcvcls of cneigr-rclated metabolitcsin intect and isolated pcrfuscd-supcrfused rrt ske|ctálmusc|e*(ŠteÍleta],1991) AdenosineS'.triphosphate(ATP), phosphocreatine(PCr), creatirre(Cr), ínorganicphosphate (Pi),lactate(LAC), pyruvate(PYR) andglycogenasglucose(GLU) weredeterminedandfree adenosineS'-diphosptrate(ADP) was calculatedtom ATP:creatinephosphokinase(CPK) reactionin the gracilis muscleof cold.acc|imatedrďs in vivo. and in completelyisoIated mrsc|esundermediumperfusionandzuperfusionínvito, rsing thefreeze-clampingmetlrcd. Themeanin vivo leve|s(pmoVgw.w.)were:ATP 4.8'PCr 12.0.Cr 7,8'Pi ló.l' LAc l.6' PYR 0.09,GLU 22.9,ADP 0,62x t0-3.[solationof the muscle(about11min of anox'a fo|lowedby perfision irrthe air with a highpo2 Inedium)decreasedmacroergicphosphďe levels(ATP 3.0.PCr 8.3).Inisolatedmusclesperfusedwitha highpO2medium(99kPaOz, perflrion rate70 pl/mitt)andsimultaneouslysupeďusedwitha low po2medium(ó.2kPao2' 2.3mVmin)at28oC in vitrothelevelsof metaboliteswere(pmoUgw.w.):ATP 3.1,PCr 8.5, Cr 5.ó.Pi 9'2, |Ac 2.|' PYR 0.l9. GLU 6.6'ADP 0.44x l0(.]).Themeansteadyoxygen upuke of the íso|atedmusclewas 97 nmol 02 x min.l x g.l w.w. Ttrus.thelevelsof macroergicphosphatesand their...
Myocardial tolerance to ischemia/reperfusion injury - possible protective mechanisms
Alánová, Petra ; Neckář, Jan (advisor) ; Nováková, Olga (referee) ; Vaněčková, Ivana (referee)
Ischemic heart disease is the leading cause of death and disability worldwide. The effects of ischemic heart disease are usually attributable to the detrimental effects of acute myocardial ischemia/reperfusion (I/R) injury. The aim of the thesis was to contribute to current effort to clarify the basis of mechanisms that could save the heart from I/R injury. The whole thesis is based on four studies; while the first three are published, the fourth one has been under revision. In the first study, we proved the involvement of nitric oxide (NO) in the cardioprotective mechanism of chronic hypoxia (CH). We described that exogenously increased availability of NO as well as inhibition of phosphodiesterase type 5 led to increased myocardial tolerance of normoxic and chronically hypoxic rats. The effects of both interventions were not additive, suggesting that NO is included in cardioprotective signaling of CH. Second study continued in investigating molecular mechanisms underlying cardioprotection induced by CH. We showed that infarct size-limiting effect of adaptation to CH was accompanied by increased myocardial concentration of tumor-necrosis factor alpha (TNF-α) and TNF-α receptor R2. In the third study, we examined the effect of dexrazoxane (DEX), the only clinically approved drug against...

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2 Nováková, Oldřiška
3 Nováková, Olga,
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