Název: A new method for double bond characterization in lipids by ultraviolet photodissociation mass spectrometry
Autoři: Rumlová, Barbora ; Strmeň, T. ; Cvačka, Josef
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Students Conference 'Modern Analytical Chemistry' /17./, Praha (CZ), 20210916
Rok: 2021
Jazyk: eng
Abstrakt: Lipids are structurally diverse biomolecules with vital functions in biological systems. Some of these functions are closely associated with the specific location of the carbon-carbon double bond in the lipid acyl chains. The chain length and the number of unsaturated carbon-carbon bonds can be determined using conventional MS/MS-based structural elucidation methods employing low or higher energy collision-induced dissociation. However, these dissociation techniques do not provide more subtle structural details, for example, the position of unsaturated bonds. Another type of activation method – ultraviolet photodissociation can be used for this purpose. In this study, a new method for characterization of double bond location in lipids acyl chain was developed using 193 nm ultraviolet photodissociation implemented on Orbitrap Fusion Lumos mass spectrometer. This approach is based on the derivatization of the double bond with bis-(5-iodo-[2]pyridyl)-disulfide, subsequent specific cleavages provide a unique diagnostic pair bearing information about the double bond position.
Klíčová slova: double bond; lipids; mass spectrometry; ultraviolet photodissociation
Zdrojový dokument: Proceedings of the 17th international students conference 'Modern Analytical Chemistry', ISBN 978-80-7444-089-2

Instituce: Ústav organické chemie a biochemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na externích webových stránkách.
Externí umístění souboru: http://web.natur.cuni.cz/analchem/isc/isc17.pdf
Původní záznam: http://hdl.handle.net/11104/0327089

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-508423


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav organické chemie a biochemie
Konferenční materiály > Příspěvky z konference