|
|
Planetarium with coffee
Vlčková, Sabina ; Třeštíková, Ing Jana (referee) ; Berková, Petra (advisor)
Thesis on "Planetarium with coffee" is processed in the form of project documentation for construction. The proposed facility is designed as a two-storeyed without basement with the main ground plan dimensions of 52,8 x 27,47 m and is located near the village Lošov close to Olomouc. The building is operationally divided into a part intended for public entrance and a part for operation and maintenance. Loadbearing structural system is reinforced concrete monolithic skeleton based on monolithic reinforced concrete foundation footings. Ceiling structure consists of reinforced concrete, monolithic, locally supported, cross reinforced floor slab. Vertical external walls are made of ceramic blocks with contact thermal insulation system. Part of the front rounded facade is designed as glass fronted. The building is covered by a warm flat roof. Roof cladding consists of vegetation layers, walkable terrace or non-walkable structure of roof cladding. The planetarium itself is covered by a reinforced concrete dome with a thermal insulation layer of plates of foamed glass. Fillings of doors and windows are made of aluminum profiles with isolating triple glazing. The building meets the requirements for barrier-free use of buildings.
|
|
|
Family House
Vlčková, Sabina ; Mičan, Přemysl (referee) ; Košíčková, Ivana (advisor)
Bachelor thesis is focused on the elaboration of project documentation for new built single-family house. This is a partial basement building with two floors. The structural system is designed as a bricked from ceramic thermal insulating blocks. The building is covered by two shed roofs with truss supporting structure.
|
|
|
Males-females differences in the spectrum of chromosomal aberrations in the group of nanocomposites production workers
Rössnerová, Andrea ; Pelcová, D. ; Ždímal, Vladimír ; Elzeinova, Fatima ; Margaryan, Hasmik ; Chvojková, Irena ; Topinka, Jan ; Schwarz, Jaroslav ; Ondráček, Jakub ; Koštejn, Martin ; Komarc, M. ; Vlčková, Š. ; Fenclová, Z. ; Lischková, L. ; Dvořáčková, Š. ; Rössner ml., Pavel
An increase in the use of nanomaterials (NM) has been witnessed in many areas of human life. Therefore, assessment of genotoxicity of NM and nanoparticles (NP) is one of the main objectives of genetic toxicology. Despite this fact, human cytogenetic studies following the exposure to NP are still rare. Moreover, no relevant information on possible differences in sensitivity to NP related to gender is available.\n\nIn this study we periodically (in September 2016, 2017 and 2018; pre-shift and post-shift each year) analyzed a group of workers (both genders), working long time in nanocomposites research, and matched controls. Aerosol exposure monitoring of particulate matter including nano-sized fractions was carried out during working shift. Micronucleus assay using Human Pan Centromeric probes, was applied to distinguish, besides the frequency of total MN in binucleated cells (BNC), also other types of chromosomal damage (losses and breaks). Moreover, whole-chromosome painting (WCP) for autosome #1 and both gonosomes (X and Y) were applied in third sampling period (2018) with the aim to identify the particular structural and numerical chromosomal aberrations.\n\nObtained results showed: (i) differences in the risk of exposure to NP related to individual working processes (welding, smelting and machining); (ii) differences in chemical composition of nano-fraction; (iii) no effect of chronic exposure of NP (total MN) opposite to significant effect of acute exposure; (iv) gender-related DNA damage differences (females seem to be more sensitive to chromosomal losses). Additional data from WCP suggested increased frequency of numerical aberrations in gonosomes.
Plný tet:  PDF
|
|
|
Biomarkers of Oxidative Stress and Inflammation in Researches Exposed to Nanoparticles by Inhalation During the Handling of Nanocomposites.
Pelclová, D. ; Ždímal, Vladimír ; Schwarz, Jaroslav ; Komarc, M. ; Vlčková, Š. ; Fenclová, Z. ; Lischková, L. ; Dvořáčková, Š. ; Rössnerová, Andrea ; Rössner ml., Pavel
At present, little is known about the health effects in the workers processing nanocomposites. In our study, 20 researchers (41.8 +/- 11.4 y/o), handling nanocomposites for 17.8 +/- 10.0 years were examined pre-shift and post-shift, together with 21 controls (42.7 +/- 11.5 y/o). Biomarkers of oxidative stress derived from lipids, nucleic acids, proteins and markers of inflammation were analyzed in the exhaled breath condensate (EBC). Aerosol exposure was monitored during three nanoparticle generation operations: smelting, welding and nanocomposite machining. Mass concentrations during these operations ranged from 0.120 to 1.840 mg/m(3), and median particle number concentrations from 4.8x10(4) to 5.4x10(5) particles/cm(3). Nanoparticles accounted for 40 to 95 % of particles, with Fe and Mn prevailing. Significant elevations were already seen in most oxidative stress markers and in several inflammation markers in the pre-shift samples relative to the controls. Significant associations were found between working in nanocomposite synthesis and the majority of EBC biomarkers. Chronic bronchitis was more frequent in researchers. A minor, but significant post-shift decrease of lung function parameters was found. We conclude that workers in nanocomposite synthesis may be at risk of developing airway disorders with time. From all the markers analyzed in EBC, the following markers were most robust and could be recommended for preventive examinations: 8-hydroxy-2-deoxyguanosine (8-OHdG) and 5-hydroxymethyl uracil (5-OHMeU) from nucleic acids, o-tyrosine (o-Tyr) and 3-nitrotyrosine (3-NOTyr) from proteins, and malondialdehyde and aldehydes C6-C13 from lipids. Among the markers of inflammation, tumor necrosis factor (TNF) and leukotriene B4 appeared to be the most useful.
Plný tet: PDF
|
|
|
DNA damage induced by occupational exposure to copper oxide nanoparticles
Rössner st., Pavel ; Pelcová, D. ; Elzeinová, Fatima ; Mikuška, Pavel ; Večeřa, Zbyněk ; Coufalík, Pavel ; Vlčková, Š. ; Fenclová, Z. ; Rössnerová, Andrea
Copper oxide nanoparticles (CuO NPs) have a widespread use in industry, chemistry, in production of electronic devices and as an antimicrobial agent. Although copper is an important biogenic element, CuO NPs are toxic with the ability to induce oxidative stress, apoptosis, cell cycle arrest or DNA damage. For humans, the inhalation route is the most common way of exposure to CuO NPs. In the body, CuO NPs may be either deposited in the lungs, or transported to other organs. Their presence usually causes oxidative stress or inflammatory responses, consequently leading to DNA damage. In this study, we investigated the effect of CuO NPs inhalation on DNA damage in a group of researches conducting animal exposure experiments. The subjects were exposed to various metal oxide nanoparticles, including CuO NPs, by inhalation for an average of 4.9 ± 0.4 years. The average mass concentration of Cu in the air during the experiment was 7.3 ± 3.2 ng/m3. Subjects not exposed to nanoparticles served as a control group. We applied micronucleus assay using Human Pan Centromeric probes to detect DNA damage and to distinguish between the frequency of centromere positive (CEN+) and centromere negative (CEN−) micronuclei (MN) in the binucleated cells. We\ndid not find differences between both groups for either mean MN frequency (10.38 ± 2.50 vs. 11.88 ± 3.01 MN/1000 binucleated cells), or CEN+/CEN- ratio (58%/42% vs. 55%/45%), for the exposed and controls, respectively. In conclusion, inhalation of CuO NPs at this low-level exposure had no effect on chromosomal losses and/or breaks.
|
|
|
Možnosti rozvoje komunitního kompostování v kraji Vysočina
Vlčková, Silvie
This thesis is focused the possibilities of development of community composting in Vysočina region. The aim of this work is to determine whethet out if it would make sense to build a community composting plant in the town of Nárameč. This decision precedes the development of the following principles. The first part deals with composting in general and legislation. The following is a description and evaluation of subplanar resources, a proposal for optimization of raw material resources, a proposal for raw material composition of coposting and an evaluation of the current state of the raw material composition. In the last part of the thesis there is pre-sented environmental, technological and economic evaluation of proposed options.
|
|
| |
|
|
Antenatal courses
Vlčková, Simona ; Chvátilová, Pavlína (advisor) ; Plecitá, Zdeňka (referee) ; Rychterová, Veronika (referee)
This essay is a study about diagnostics and signs of pregnancy, its durations, calculation of the birth date and changes of mother's body during pregnacy. The essay contains instructions about diet and recommended energetic dosage during pragnancy, a special attention is on vitamins, calcium and folic acid. Also we discuses the importance of exercising,rules and when is it recommended the most. Also it talks about how the pregnant woman should take care about her teeth, and advisable hygienic preparations. A special attention is give to atenatal courses. The essay is talking about the reason why antenatal courses are mportent for pregnant woman, how to choose rightly and what kind of information can they get there. A questionnaire is a part of the essay, which is discussing the anteatal preparations and their affect on pregnancy, complications, birth injury, and the affect of educatig mothers to participate in such courses.
|
|
|
Mercury and its Future in the Czech Republic
Navrátil, Tomáš ; Vytopilová, M. ; Vlčková, Š. ; Mrázová, K. ; Zakharov, S. ; Honsová, Š. ; Pelclová, D.
Toxicity of mercury has been discussed for many years by specialists as well as by laymen. Legislative tools connected with measures and conditions concerning the trade, manufacture, use and interim storage of mercury, mercury compounds, mixtures, mercury-added products and the management of mercury waste are currently being prepared. Some suggestions and prepared measurements are mentioned and discussed in this contribution. Of course, it is necessary to differentiate among various forms of it, i.e., metallic form, vapors, amalgams, soluble and insoluble inorganic and organic compounds.
|
|
|
Planetarium with coffee
Vlčková, Sabina ; Třeštíková, Ing Jana (referee) ; Berková, Petra (advisor)
Thesis on "Planetarium with coffee" is processed in the form of project documentation for construction. The proposed facility is designed as a two-storeyed without basement with the main ground plan dimensions of 52,8 x 27,47 m and is located near the village Lošov close to Olomouc. The building is operationally divided into a part intended for public entrance and a part for operation and maintenance. Loadbearing structural system is reinforced concrete monolithic skeleton based on monolithic reinforced concrete foundation footings. Ceiling structure consists of reinforced concrete, monolithic, locally supported, cross reinforced floor slab. Vertical external walls are made of ceramic blocks with contact thermal insulation system. Part of the front rounded facade is designed as glass fronted. The building is covered by a warm flat roof. Roof cladding consists of vegetation layers, walkable terrace or non-walkable structure of roof cladding. The planetarium itself is covered by a reinforced concrete dome with a thermal insulation layer of plates of foamed glass. Fillings of doors and windows are made of aluminum profiles with isolating triple glazing. The building meets the requirements for barrier-free use of buildings.
|
guest ::
