Original title: DNA damage induced by occupational exposure to copper oxide nanoparticles
Authors: Rössner st., Pavel ; Pelcová, D. ; Elzeinová, Fatima ; Mikuška, Pavel ; Večeřa, Zbyněk ; Coufalík, Pavel ; Vlčková, Š. ; Fenclová, Z. ; Rössnerová, Andrea
Document type: Papers
Conference/Event: NANOCON 2019. International Conference on Nanomaterials - Research & Application /11./, Brno (CZ), 20191016
Year: 2020
Language: eng
Abstract: Copper oxide nanoparticles (CuO NPs) have a widespread use in industry, chemistry, in production of electronic devices and as an antimicrobial agent. Although copper is an important biogenic element, CuO NPs are toxic with the ability to induce oxidative stress, apoptosis, cell cycle arrest or DNA damage. For humans, the inhalation route is the most common way of exposure to CuO NPs. In the body, CuO NPs may be either deposited in the lungs, or transported to other organs. Their presence usually causes oxidative stress or inflammatory responses, consequently leading to DNA damage. In this study, we investigated the effect of CuO NPs inhalation on DNA damage in a group of researches conducting animal exposure experiments. The subjects were exposed to various metal oxide nanoparticles, including CuO NPs, by inhalation for an average of 4.9 ± 0.4 years. The average mass concentration of Cu in the air during the experiment was 7.3 ± 3.2 ng/m3. Subjects not exposed to nanoparticles served as a control group. We applied micronucleus assay using Human Pan Centromeric probes to detect DNA damage and to distinguish between the frequency of centromere positive (CEN+) and centromere negative (CEN−) micronuclei (MN) in the binucleated cells. We\ndid not find differences between both groups for either mean MN frequency (10.38 ± 2.50 vs. 11.88 ± 3.01 MN/1000 binucleated cells), or CEN+/CEN- ratio (58%/42% vs. 55%/45%), for the exposed and controls, respectively. In conclusion, inhalation of CuO NPs at this low-level exposure had no effect on chromosomal losses and/or breaks.
Keywords: copper oxide nanoparticles; DNA damage; inhalation
Project no.: GA18-02079S (CEP), LO1508 (CEP)
Funding provider: GA ČR, GA MŠk
Host item entry: NANOCON Conference Proceedings - International Conference on Nanomaterials, ISBN 978-808729495-6, ISSN 2694-930X
Note: Související webová stránka: https://www.confer.cz/nanocon/2019

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0311191

Permalink: http://www.nusl.cz/ntk/nusl-432260


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers
 Record created 2020-12-03, last modified 2023-12-06


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