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Polyphasic approaches in the taxonomy of green aerophytic algae
Škaloud, Pavel ; Neustupa, Jiří (advisor) ; Lukešová, Alena (referee) ; Rindi, Fabio (referee)
Conclusions Traditionaltaxonomyoftendependson single charactersusedfor distin- guishingparticularspecies.ln thisthesis,I haveillustratedapplicationofpoly- phasicapproachesin taxonomyof greenaerophyticalgae.Theseapproachesare basedon thecombinationof variouscharacters(traditionalmorphology,modern molphologicalapproaches,SSU, ITS rDNA andgeneintronsequencing)forthe speciesrecognitionin greenalgae. The morphologicalcharactersaloneareoftennot sufficientfor speciesde- lirnitation,thoughtheycan be usedfor routinespeciesidentificationModern rnorphologicalapproacheslike confocalmicroscopyallow to detectclearmor- phologicaldifferencesarnonginvestigatedcloselyrelatedspecies,evenifat first sightthesemorphologicallyvery uniformalgaehardlydifferatthe morphologi- cal level. A numberof rnorphologicalcharactersdoesnot reflectgeneticrela- tionships.Rathertheyareinfluencedby variousenvironmentalor culturecondi- tions.For example,cell dimensionsin Klebsorntidiuntcanbe distinctivelyinflu- encedby pH, humidity,illLrminationandternperatureconditions.However,with theaid of molecularmarkerswe candetectproperrnorphologicalfeaturesuseful for speciesdifferentiationt. ln .lslerochlorls,correlationtestof geneticdistances and morphologicaldifferencesrevealedusefulnessof particularchloroplast charactersforspeciesdelirnitation....
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Soil crust and its role in erosion of post mining sites
Fiedlerová, Jana ; Frouz, Jan (advisor) ; Lukešová, Alena (referee)
9 2 Abstract The object of the study was the soil crust, found on spoil tips after coal mining near Sokolov. Certain type of crusts were compared; non-biological, physico-chemical origin and biological crusts that are made up of algae, mosses and lichens. Individual samples of the crusts underwent the laboratory analysis - Water Drop Penetration Time, Sequencing Electron Microscopy, water infiltration, and field studies, where the measurements were taken by using the erosion pins, the surface of the crusts was evaluated and photographed stereoscopically. The results indicate that the the physical-chemical type of crusts show significantly greater erosion, while the biological crusts are less susceptible to erosion, this phenomenon is probably related to the mechanical firming of the surface. Keywords: Soil crust, Mining soil, Mining, Infiltration, Erosion
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Vliv hospodářských zásahů na změnu v biologické rozmanitosti ve zvláště chráněných územích: Zhodnocení vlivu hospodářských zásahů na diverzitu půdních biot ve vybraných zvláště chráněných územích
Ústav půdní biologie AV ČR, České Budějovice ; Tajovský, Karel ; Starý, Josef ; Nováková, Alena ; Lukešová, Alena ; Háněl, Ladislav ; Balík, Vladimír ; Pižl, Václav
Cílem dílčího projektu, jehož řešení je rozvrženo do čtyř let (2000-2003), je shromáždění základních poznatků o diverzitě modelových skupin půdních organismů na lokalitách Trojmezná a Smrčina, NPR Boubín a NPR Žákova hora, a vyhodnocení vlivu hospodářských zásahů na půdní biotu v těchto územích. Syntéza získaných poznatků slouží k navržení vhodného managementu porostů vedoucímu k zachování a ochraně biodiverzity. Tato zpráva je shrnutím výsledků v roce 2000 a 2001. Materiál a data z podzimních odběrů v roce 2001 jsou v případě některých skupin půdních biot ve fázi zpracování či následných analýz a nemohly být vzhledem k termínu odevzdání do této zprávy zahrnuty.
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Interactions between the soil micro-flora and invertebrates in Slovak and Moravian caves
Lukešová, Alena ; Nováková, Alena
The role of microflora as food for soil invertebrates is well documented. However, data on interactions between communities of organisms living in caves are very limited. Three caves of the Slovak Karst NP (Domica Cave, Dlhá Chodba Cave, Ardovská Cave) and the Amatérská Cave (Moravian Karst) were selected for this study because from visual observations, it was clear that there was some invertebrate activity. The aim of the study was to compare the communities of algae, cyanobacteria and microscopic fungi developing in excrements of cave invertebrates and in the surrounding cave sediment. The richest communities of both algae and microfungi were found in earthworm casts, containing up to two fold more species than in the surrounding sediment (73 species of microscopic fungi in the casts compared to 37 in surrounding cave sediment in the Domica Cave, and 45 vs. 27 species of algae in the Dlhá Chodba Cave).
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Microscopic fungi as food of cave invertebrate animals - a laboratory food preference experiment
Nováková, Alena ; Luptáčik, P. ; Kováč, L. ; Lukešová, Alena ; Šustr, Vladimír
The aim was to improve the methodology of the multi-choice food preference tests as a tool for investigation of feeding preference of cave invertebrates. Fungi and algae isolated from cave sediments of the Domica Cave system (NP Slovak Karst) were offered in cafeteria tests as a food to springtail .i.Folsomia candida./i., mites .i.Pantelozetes cavatica, Scheloribates pallidulus./i., and isopod .i.Mesoniscus graniger. M. graniger./i. preferred some species of algae, all offered fungi were rejected. Mainly algae and .i.Mucor./i. spp. from microscopic fungi are preferred by .i.S. pallidulus./i.. Preference of .i.Mucor./i. spp. was observed by cave mite .i.P. cavatica./i. and cave population of .i.F. candida./i. too. All cave animals showed low preference for offered food and have tendency to stay rather on clay cave sediment then on the food.
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Vliv kultivačních podmínek na kryoprezervaci půdních řas a sinic
Lukešová, Alena
Fundamental principles of both conservative and vitrification cryopreservation methods are presented, and factors affecting cryopreservation of algae and cyanobacteria are summarized. These factors include factors connected with the cryopreservation process itself as well as factors connected with pre- and post-cryopreservation manipulation with samples. Attention is paid to cultivation conditions, mainly to the temperature in the context of application of cold acclimation before cryopreservation. Both literature data and our experience indicate that the response of soil algae and cyanobacteria to particular factors is species or strain specific and optimization of condition can enhance post-cryopreservation viability of cryopreservation recalcitrant strain.
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Potravní zdroje bezobratlých v Ardovské jeskyni a v jeskyni Domica - předběžné výsledky
Nováková, Alena ; Elhottová, Dana ; Krištůfek, Václav ; Lukešová, Alena ; Hill, P. ; Kováč, L. ; Mock, A. ; Luptáčik, P.
The chemistry (organic matter content and pH) of different substrates from the Ardovská and Domica Caves in the Slovak Karst National Park (Slovakia) and the composition of their microbial communities were studied. Differences in organic matter content and microbial communities were found between the substrates from the Domica and Ardovská Caves. A broad spectrum of photoautotrophic microorganisms (algae and cyanobacteria) was found in different cave microhabitats (cave sediment, rotten wood, earthworms casts and isopods faeces), but no algae were detected in the fresh bat droppings and in the intestinal tract of the isopod .i.M. graniger./i.. Microfungi and bacterial colonies play the primary role in transforming bat guano into a food source for saprophagous and microphytophagous invertebrates.
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Soil microflora of spruce forests of the Bohemian Forest
Nováková, Alena ; Lukešová, Alena
Species composition and quantity of microalgae and micromycetes inhabiting soils of original spruce forest were studied in 3 localities of the Bohemian Forest (Trojmezná, Smrčina, Boubín) in years 2000-2003. Both intact plots, and those ones with selective tree cutting were compared in Trojmezná and Smrčina. In total, 46 species of algae and 72 taxa of micromycetes, represented by common soil species, were found in studied soils. Green algae (mainly Chlorophyceae and Charophyceae) prevailed both qualitatively and quantitatively among soil algae which was connected with low pH. All isolated micromycetes belonged to saprotrophic fungi. Total numbers of algal species ranged between 20-33 and total algal abundance between 48,1-4210,0 cells per g of dry soil in studied plots. Higher algal species richness was recorded in plots with selective tree cutting then in intact plots. Oposite trend of average total algal abundance was observed, despite big variations in particular sampling occasions. Numbers of taxa of saprophytic mycromycetes ranged between 32-40, lenght of mycelium between 6,1-84,5 m per g of dry soil, and numbers of CFU between 57,5-2413,4. No differences in species composition and quantity of saprotrophic micromycetes between intact plots and plots with tree cutting were found.
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Potential sources of polyunsaturated fatty acids for saprophagous soil invertebrates
Elhottová, Dana ; Frouz, Jan ; Krištůfek, Václav ; Lukešová, Alena ; Nováková, Alena ; Tříska, Jan
Main sources of important nutritionally compounds - polyunsaturated fatty acids (PUFA) essential in soils animal diet were compared. The highest concentration of PUFA was found in cyanobacteria (namely Antarctic strain Pseudophormidium sp.), followed by fresh litter, algae, fungi and decomposed litter. The PUFA occurrence in bacteria including actinomycetes was rare. Leaf litter is the most important source of PUFA for soil invertebrates in regards to both PUFA concentration and occurrence in soil. The stage of litter decomposition significantly affected the content of all 13 detected PUFA. The total PUFA content decreased significantly during litter decomposition. Enrichment in certain PUFA was observed in the latest stages of litter decomposition, indicating that the soil microflora improves the nutritional value of the decaying ĺitter.
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