|
|
Polyphasic approaches in the taxonomy of green aerophytic algae
Škaloud, Pavel ; Neustupa, Jiří (vedoucí práce) ; Lukešová, Alena (oponent) ; Rindi, Fabio (oponent)
Conclusions Traditionaltaxonomyoftendependson single charactersusedfor distin- guishingparticularspecies.ln thisthesis,I haveillustratedapplicationofpoly- phasicapproachesin taxonomyof greenaerophyticalgae.Theseapproachesare basedon thecombinationof variouscharacters(traditionalmorphology,modern molphologicalapproaches,SSU, ITS rDNA andgeneintronsequencing)forthe speciesrecognitionin greenalgae. The morphologicalcharactersaloneareoftennot sufficientfor speciesde- lirnitation,thoughtheycan be usedfor routinespeciesidentificationModern rnorphologicalapproacheslike confocalmicroscopyallow to detectclearmor- phologicaldifferencesarnonginvestigatedcloselyrelatedspecies,evenifat first sightthesemorphologicallyvery uniformalgaehardlydifferatthe morphologi- cal level. A numberof rnorphologicalcharactersdoesnot reflectgeneticrela- tionships.Rathertheyareinfluencedby variousenvironmentalor culturecondi- tions.For example,cell dimensionsin Klebsorntidiuntcanbe distinctivelyinflu- encedby pH, humidity,illLrminationandternperatureconditions.However,with theaid of molecularmarkerswe candetectproperrnorphologicalfeaturesuseful for speciesdifferentiationt. ln .lslerochlorls,correlationtestof geneticdistances and morphologicaldifferencesrevealedusefulnessof particularchloroplast charactersforspeciesdelirnitation....
|
host ::
RSS.