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Measuring communication using the Amec Integrated Evaluation Framework on the example of the non-profit organization Czechitas
Jeřábková, Kateřina ; Ježková, Tereza (advisor) ; Rosenfeldová, Jana (referee)
Measurement and evaluation of public relations and communication is the main topic of the thesis. This topic resonates strongly among academics and practitioners, especially in the last decade. However, there is still a significant discrepancy between what the theory recommends and the acceptance of these recommendations in practice. Therefore, the thesis focuses on one specific framework for measuring communication and, using a case study method, determines the applicability of this framework in practice. The aim of the research, which is carried out using the case study method, is to verify the applicability of the Integrated Evaluation Framework (IEF) in measuring and evaluating the communication of the non-profit organization Czechitas. In the theoretical chapters, the thesis offers the definition of PR and the main areas PR influence in organizations (image, reputation, identity, and brand). It furher describes the Czech non-profit sector and the specifics of it's marketing and PR activities. It presents the best-known theoretical models of PR measurement and evaluation as well as the progressthe debate on PR measurement among experts has seen in recent years. The analytical part of the thesis analyzes the selected case for the study, being the Czech non-profit organization Czechitas. The analysis of...
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The roles of Trim15 and UCHL3 in the ubiquitin-mediated cell cycle regulation.
Jeřábková, Kateřina ; Chawengsaksophak, Kallayanee (advisor) ; Šolc, Petr (referee) ; Thorpe, Peter (referee)
(ENGLISH) Ubiquitin signaling is a key regulatory mechanism for many important cellular processes such as transcription, differentiation and cell division. Cell division requires duplication of all genetic material during S-phase followed by its precise partitioning between two daughter cells during mitosis. Misregulation of the complex mitotic machinery may lead to aneuploidy and genomic instability, known drivers of tumorigenesis. Indeed, systematic genetic analysis of many cancer tissues over the last decades, indicates the presence of severe chromosome abnormalities in thousands of cancer tissue samples. In this work, I investigated the function of two components of ubiquitin signaling, the deubiquitinating enzyme UCHL3 and the E3 ubiquitin ligase TRIM15. The hypothesized role of E3 ligase TRIM15 in the cell cycle regulation could not be confirmed by our experiments, but I observed an effect on cell adhesion and motility instead. UCHL3 was identified using high-content visual siRNA screen, as a critical factor controlling genome segregation and integrity. Interestingly, it has been previously reported that UCHL3 levels are altered in various cancer types, especially colon cancer. My data demonstrate that UCHL3 drives proper alignment of chromosomes at the metaphase plate by facilitating...
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Current Trends in Arts Marketing: Example of National Theatre
Jeřábková, Kateřina ; Klimeš, David (advisor) ; Ježková, Tereza (referee)
This thesis reviews current theory and practice of audience development. It examines the effectiveness of audience development and ways of embedding it into marketing theory. The analytical part of this thesis presents how three theatre institutions employ audience development - the National Theatre, Na Zábradlí Theatre, and A Studio Rubín - as viewed by their respective marketing experts. It further analyses audiences' responses to special events, the most common format of audience development. It discusses the specifics of arts marketing, with a special focus on theatre marketing, and aim to define differences in marketing strategies between institutions of different status, financial structure, and size. In addition to discussing current and previous trends in audience development in the Czech Republic and abroad, it provides an overview of the theoretical debate on the topic. The thesis then presents field research into audience engagement activities from the perspective of their creators, but also examine how they are received by audiences, including how they affect theatres' public image and customer behaviour.
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The roles of Trim15 and UCHL3 in the ubiquitin-mediated cell cycle regulation.
Jeřábková, Kateřina ; Chawengsaksophak, Kallayanee (advisor) ; Šolc, Petr (referee) ; Thorpe, Peter (referee)
(ENGLISH) Ubiquitin signaling is a key regulatory mechanism for many important cellular processes such as transcription, differentiation and cell division. Cell division requires duplication of all genetic material during S-phase followed by its precise partitioning between two daughter cells during mitosis. Misregulation of the complex mitotic machinery may lead to aneuploidy and genomic instability, known drivers of tumorigenesis. Indeed, systematic genetic analysis of many cancer tissues over the last decades, indicates the presence of severe chromosome abnormalities in thousands of cancer tissue samples. In this work, I investigated the function of two components of ubiquitin signaling, the deubiquitinating enzyme UCHL3 and the E3 ubiquitin ligase TRIM15. The hypothesized role of E3 ligase TRIM15 in the cell cycle regulation could not be confirmed by our experiments, but I observed an effect on cell adhesion and motility instead. UCHL3 was identified using high-content visual siRNA screen, as a critical factor controlling genome segregation and integrity. Interestingly, it has been previously reported that UCHL3 levels are altered in various cancer types, especially colon cancer. My data demonstrate that UCHL3 drives proper alignment of chromosomes at the metaphase plate by facilitating...
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Effect of long-term application of morphine on opioid receptors in rat brain cortex
Jeřábková, Kateřina ; Hejnová, Lucie (advisor) ; Roubalová, Lenka (referee)
-5- ABSTRACT A huge effort has been put in determining the mechanism of the development of tolerance and dependence in context of clinical use of morphine for treatment of severe pain. Understanding of this mechanism would help to design new and more efficient pharmaceuticals. This diploma paper discus the opiate receptors with a special focus on long-term effect of chronic morphine treatment, which was determined using a radioligand binding assays with a non-selective antagonist [3 H]Diprenorphine. One of the goals of this work was to create and optimise a method for preparation of pure plasma membranes from rat cortex using percoll gradient. There were five groups, which differed in the length of morphine treatment: ten days (M-10), twenty-eight days (M-28), ten days with seven days of regression (RM-10 twenty-eight days with seven days of regression (RM-28) and a control group (K). The loss of total opioid receptor number was noticeable after ten days and grew slightly during continuous morphine treatment and kept lowering in the period of regression. The total loss was approximately 30% of the control binding. The equilibrium dissociation constant (Kd), thus the affinity of [3 H]Diprenorphine wasn't significantly different among the groups. Morphine acts through µ-opioid receptor, that's why there was a...
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Characterization of μ-type opioid receptor in CNS
Jeřábková, Kateřina ; Rudajev, Vladimír (referee) ; Hejnová, Lucie (advisor)
The topic of the opioid receptors was analyzed in this work, particularly the µ-type. The whole research was divided into two parts - the theoretical summary and the experimental part. The main goal of the theoretical part was to summarize the known facts about µ-type of opioid receptors from the morphological and pharmacological point of view. The stress was also laid on the interactions between µ receptor and the other types. The main goal of the experimental part was to determine the total number of the opioid receptors of all three types in the rat cortex, thus assess the total number of binding sites in this tissue. This assignment was performed by the binding experiments with radiolabeled ligands. The specific bound of the nonselective antagonist [3 H]diprenorphine was observed. This experiment was carried out in the sample of brain tissue from the cortex of an adult rat. The maximum binding capacity of this tissue was determined to be 236 fmol in a milligram of proteins and the equilibrium dissociation constant was 149 pmol dm-3 . All of the performed experiments were carried out in the saturation layout and were analyzed by the nonlinear fit of the data.
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Film Notes
Jeřábková, Kateřina ; Trajkov, Ivo (advisor) ; Daňhel, Jan (referee)
Structure of time space and a system of relations. This text deals with framing, off-space, characters, audience and other elements of film structure. It is mainly about relations between these aspects.
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