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Personal data protection with focus on processing and protection in civil aviation
Hodek, Jan ; Scheu, Harald Christian (advisor) ; Svobodová, Magdaléna (referee)
Personal Data Protection with Focus on Processing and Protection in Civil Aviation This diploma thesis focuses on the matter of EU legal regulation of personal data protection and reflects it in the field of civil aviation with focus on commercial air transport, as commercial air transport is at this moment the area of civil aviation where most of the personal data processing and operations with them are made. The goals of this diploma thesis are mainly to describe respective personal data processing in civil aviation and finding out whether GDPR represents an insurmountable legal obstacle for air carriers and civil aviation to operate or whether it is just another complicated act of legislation that air carriers and other entities in civil aviation must reflect. The first chapter of the thesis briefly introduces the history of personal data protection while presenting some of its past and current sources of legislation. Moreover, the first chapter introduces certain legal institutes of current EU personal data protection legislation and draws attention to the continuity and discontinuity of GDPR with the previous one. The second chapter of the thesis provides a short introduction to the topic of civil aviation legislation and describes the relationship between this legislation and GDPR. The...
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Personal Data Protection with Focus on Processing and Protection in Civil Aviation
Hodek, Jan ; Svoboda, Pavel (referee)
Personal Data Protection with Focus on Processing and Protection in Civil Aviation This diploma thesis focuses on the matter of EU legal regulation of personal data protection and reflects it in the field of civil aviation with focus on commercial air transport, as commercial air transport is at this moment the area of civil aviation where most of the personal data processing and operations with them are made. The goals of this diploma thesis are mainly to describe respective personal data processing in civil aviation and finding out whether GDPR represents an insurmountable legal obstacle for air carriers and civil aviation to operate or whether it is just another complicated act of legislation that air carriers and other entities in civil aviation must reflect. The first chapter of the thesis briefly introduces the history of personal data protection while presenting some of its past and current sources of legislation. Moreover, the first chapter introduces certain legal institutes of current EU personal data protection legislation and draws attention to the continuity and discontinuity of GDPR with the previous one. The second chapter of the thesis provides a short introduction to the topic of civil aviation legislation and describes the relationship between this legislation and GDPR. The...
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Development and validation of methods for GMO detection
Hodek, Jan ; Ovesná, Jaroslava (advisor) ; Konopásek, Ivo (referee) ; Pazlarová, Jarmila (referee)
The present work is focused on the issue of genetically modified organisms (GMOs) detection in the plants and derived products. Existing legislation in the European Union regulates monitoring of GMO and lays down rules for their labeling and traceability. The aim was to contribute to solving certain issues of critical points in GMO detection. The first point was DNA extraction. The problem was adressed to the example of DNA extraction from the fruit of papaya and candied papaya. For both food products the suitability of the selected extraction methods was verified and confirmed by amplification of a specific DNA sequence by PCR. The second critical point was related to PCR inhibitors. We observed the effect of real-time PCR inhibition due to the influence of the residual EDTA and the residual amount of heavy metal ions, which were present in laboratory plastic. In both examples, the inhibition affects the PCR efficiency. Another critical point in the GMO detection is use of screening elements. That work was focused on false positive results. Development of new methods for GMO detection was another goal of this work. We have developed the method for garden pea taxon-specific gene quantification by real-time PCR. The method was tested on 13 cultivars of garden pea and several related plants of...
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Methodology for analysis of dairy products and their soy analogues for the presence of RoundUp Ready soybean
Tesařová, Zuzana ; Šídová, Dana ; Vráblík, Aleš ; Hodek, Jan ; Ovesná, Jaroslava
Dairy products may be subject to adulteration, where there is a relatively expensive raw material (e.g. cream) replaced by the addition of vegetable fats. Milk can be replaced in dairy products for example by soybean extract. Because soy belongs among strong allergens and the incidence of allergy is rather high. Therefore it is necessary to have a method for its detection. This work introduces the method for detection of soy, including analysis of genetic modified RoundUp Ready soybean. Detection of soybean in dairy products is based on analysis of amplification products melting profiles by High Resolution Melting (HRM) method. Analysis of geneticaly modified RoundUp Ready soybean is based on DNA detection via Polymerase Chain Reaction(PCR).
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Zabezpečení laboratorní detekce geneticky modifikovaných organismů
Výzkumný ústav rostlinné výroby‚ Praha-Ruzyně ; Koucká, Petra ; Hodek, Jan ; Pouchová, Vladimíra ; Kučera, Ladislav ; Ovesná, Jaroslava
Podklady pro metodiku vzorkování rostlinných materiálů pro potřeby správních úřadů. Zpracování informací z ENGL pro potřeby ČIŽP. Shromažďování referenčních materiálů. Metodiky stanovení GMO. Svazek obsahuje sborník na téma Geneticky modifikované organismy - současnost, rozšíření a možné interakce s životním prostředím. Jednotlivé příspěvky ve sborníku: geneticky modifikované organismy - základní informace, Možnosti stanovení GMO, Křižitelnost plodin a planých druhů jako potenciál pro únik genetických modifikací, Šíření pylu v porostech lnu setého, Problematika GM hrachu v ČR, Perspektivy využití GMO, Problematika GM bramboru v ČR.
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PCR based assay for detection garden pea lec gene
Vráblík, Aleš ; Hodek, Jan ; Ovesná, Jaroslava
The method was developed and verified by staff of National reference laboratory for GMO identification and DNA fingerprinting suited for governmental control laboratories and private laboratories that analyze food and feed derived from various plant matrices. Method can be applied for quantification of GM pea in a sample, when reference gene is used as a standard. The method describe internal garden pea specific gene, lektin in this case, detection using PCR and real-time PCR. The general principle of the assay relies on lektin specific sequence presence that represents a species specific gene of garden pea. In many matrices DNA is damaged so amplification of short stretches of DNA is used. After amplification of target exploiting newly developer species specific gene primers PCR products are separated by electrophoresis in agarose gel. Resulting band is visualized by UV light and its position is compare with size standard. Alternatively, real-time PCR and ABI platform in combination with SYBR® Green can be used. Reaction parameters are described and specificity of reaction was verified. LOD as well as LOQ was defined.
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Methods of DNA extraction from the fresh papaya fruit and from the candied
Ovesná, Jaroslava ; Hodek, Jan
Aim the work is to provide a working procedure for extraction of amplifiable DNA form papaya fruits (Carica papaya), which are a poor source of DNA. Methods are optimised for DNA extraction from papaya fruits and stones and from candied papaya- these both were predicted as suitable commodities for an eventually screening of non-approved GM papaya in the market of Czech Republic. The methodology was prepared on basis of demand of reference laboratories of public service, which are involved in GMO analyses and also as a flow-work of Methodics for Detection of GM papaya 55-1 and 63-1 (Hodek, Ovesná, Pavlátová 2008) and scientific publication Detection of transgenic papaya lines: extraction protocol optimisation and verification of DNA quality by PCR assay (Ovesná, Hodek 2009).
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